Restriction map for
pF1KB7920
(Linear) (Six-base) MAPSORT of: KIBB7920.seq Check: 4564 from: 1 to: 1038
KIBB7920.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 752 1038
Size: 752 286
AccI GT'mk_AC
Cuts at: 0 505 1038
Size: 505 533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1038
Size: 831 207
ApoI r'AATT_y
Cuts at: 0 141 233 1038
Size: 141 92 805
Fragments arranged by size:
805 141 92
AvaI C'yCGr_G
Cuts at: 0 899 1038
Size: 899 139
BamHI G'GATC_C
Cuts at: 0 524 1038
Size: 524 514
BanI G'GyrC_C
Cuts at: 0 249 1038
Size: 249 789
BanII G_rGCy'C
Cuts at: 0 335 731 1038
Size: 335 396 307
Fragments arranged by size:
396 335 307
BbvCI CC'TCA_GC
Cuts at: 0 245 749 1038
Size: 245 504 289
Fragments arranged by size:
504 289 245
BciVI GTATCCnnnnn_n'
Cuts at: 0 834 1038
Size: 834 204
BlpI GC'TnA_GC
Cuts at: 0 599 1038
Size: 599 439
Bme1580I G_kGCm'C
Cuts at: 0 565 1038
Size: 565 473
BmtI G_CTAG'C
Cuts at: 0 244 1038
Size: 244 794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 409 1038
Size: 202 207 629
Fragments arranged by size:
629 207 202
Bpu10I CC'TnA_GC
Cuts at: 0 245 749 1038
Size: 245 504 289
Fragments arranged by size:
504 289 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1038
Size: 745 293
BsaAI yAC'GTr
Cuts at: 0 489 1038
Size: 489 549
BsaWI w'CCGG_w
Cuts at: 0 100 863 1038
Size: 100 763 175
Fragments arranged by size:
763 175 100
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 766 1038
Size: 766 272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1038
Size: 811 227
BsmBI CGTCTCn'nnnn_
Cuts at: 0 427 1038
Size: 427 611
Bsp1286I G_dGCh'C
Cuts at: 0 335 565 731 1038
Size: 335 230 166 307
Fragments arranged by size:
335 307 230 166
BspEI T'CCGG_A
Cuts at: 0 863 1038
Size: 863 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 635 752 1038
Size: 635 117 286
Fragments arranged by size:
635 286 117
BsrDI GCAATG_nn'
Cuts at: 0 72 212 1038
Size: 72 140 826
Fragments arranged by size:
826 140 72
BsrFI r'CCGG_y
Cuts at: 0 998 1038
Size: 998 40
BsrGI T'GTAC_A
Cuts at: 0 281 1038
Size: 281 757
BstAPI GCAn_nnn'nTGC
Cuts at: 0 214 1038
Size: 214 824
BstYI r'GATC_y
Cuts at: 0 524 1038
Size: 524 514
BstZ17I GTA'TAC
Cuts at: 0 506 1038
Size: 506 532
BtgI C'CryG_G
Cuts at: 0 731 1038
Size: 731 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 579 1038
Size: 579 459
DraIII CAC_nnn'GTG
Cuts at: 0 501 778 1038
Size: 501 277 260
Fragments arranged by size:
501 277 260
EaeI y'GGCC_r
Cuts at: 0 612 815 1038
Size: 612 203 223
Fragments arranged by size:
612 223 203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 409 831 1038
Size: 202 207 422 207
Fragments arranged by size:
422 207 207 202
EcoO109I rG'GnC_Cy
Cuts at: 0 273 1038
Size: 273 765
EcoRI G'AATT_C
Cuts at: 0 233 1038
Size: 233 805
EcoRV GAT'ATC
Cuts at: 0 588 1038
Size: 588 450
HaeII r_GCGC'y
Cuts at: 0 1005 1038
Size: 1005 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 303 335 393 425 1038
Size: 160 32 111 32 58 32 613
Fragments arranged by size:
613 160 111 58 32 32 32
HincII GTy'rAC
Cuts at: 0 935 1038
Size: 935 103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 1038
Size: 594 444
MscI TGG'CCA
Cuts at: 0 614 817 1038
Size: 614 203 221
Fragments arranged by size:
614 221 203
MslI CAynn'nnrTG
Cuts at: 0 167 1038
Size: 167 871
MspA1I CmG'CkG
Cuts at: 0 603 748 1038
Size: 603 145 290
Fragments arranged by size:
603 290 145
NaeI GCC'GGC
Cuts at: 0 1000 1038
Size: 1000 38
NdeI CA'TA_TG
Cuts at: 0 515 1038
Size: 515 523
NgoMIV G'CCGG_C
Cuts at: 0 998 1038
Size: 998 40
NheI G'CTAG_C
Cuts at: 0 240 1038
Size: 240 798
PasI CC'CwG_GG
Cuts at: 0 531 1038
Size: 531 507
PpuMI rG'GwC_Cy
Cuts at: 0 273 1038
Size: 273 765
PvuII CAG'CTG
Cuts at: 0 603 748 1038
Size: 603 145 290
Fragments arranged by size:
603 290 145
ScaI AGT'ACT
Cuts at: 0 983 1038
Size: 983 55
SexAI A'CCwGG_T
Cuts at: 0 774 1038
Size: 774 264
SfcI C'TryA_G
Cuts at: 0 412 1038
Size: 412 626
SmaI CCC'GGG
Cuts at: 0 901 1038
Size: 901 137
SmlI C'TyrA_G
Cuts at: 0 724 1038
Size: 724 314
StyI C'CwwG_G
Cuts at: 0 378 801 1038
Size: 378 423 237
Fragments arranged by size:
423 378 237
TatI w'GTAC_w
Cuts at: 0 159 281 981 1038
Size: 159 122 700 57
Fragments arranged by size:
700 159 122 57
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 834 1038
Size: 834 204
XmaI C'CCGG_G
Cuts at: 0 899 1038
Size: 899 139
XmnI GAAnn'nnTTC
Cuts at: 0 142 1038
Size: 142 896
Enzymes that do cut:
AarI AccI AcuI ApoI AvaI BamHI BanI BanII
BbvCI BciVI BlpI Bme1580I BmtI BpmI Bpu10I BpuEI
BsaAI BsaWI BseRI BsgI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrFI BsrGI BstAPI BstYI BstZ17I BtgI BtgZI
DraIII EaeI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NdeI NgoMIV
NheI PasI PpuMI PvuII ScaI SexAI SfcI SmaI
SmlI StyI TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BcgI BclI BfrBI
BglI BglII BmgBI BmrI BplI BsaI BsaBI BsaHI
BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BspHI BsrBI
BssHII BssSI BstBI BstEII BstXI Bsu36I BtsI ClaI
CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XhoI ZraI