(Linear) (Six-base) MAPSORT of: KIBB7921.seq  Check: 6296  from: 1  to: 543



KIBB7921.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     403     543 
   Size:        269     134     140

  Fragments arranged by size: 

                 269     140     134
 
AflIII A'CryG_T

Cuts at:      0     454     543 
   Size:        454      89
 
AlwNI CAG_nnn'CTG

Cuts at:      0     196     543 
   Size:        196     347
 
AvaI C'yCGr_G

Cuts at:      0     214     543 
   Size:        214     329
 
BanII G_rGCy'C

Cuts at:      0     404     471     543 
   Size:        404      67      72

  Fragments arranged by size: 

                 404      72      67
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     334     543 
   Size:        334     209
 
BbvCI CC'TCA_GC

Cuts at:      0     133     543 
   Size:        133     410
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     543 
   Size:        470      73
 
Bpu10I CC'TnA_GC

Cuts at:      0     133     543 
   Size:        133     410
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     543 
   Size:        388     155
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      30     543 
   Size:         30     513
 
BsaAI yAC'GTr

Cuts at:      0     455     543 
   Size:        455      88
 
BsaHI Gr'CG_yC

Cuts at:      0     112     543 
   Size:        112     431
 
BsaWI w'CCGG_w

Cuts at:      0     167     543 
   Size:        167     376
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     543 
   Size:        412     131
 
BsiHKAI G_wGCw'C

Cuts at:      0     404     543 
   Size:        404     139
 
BsmI GAATG_Cn'

Cuts at:      0     378     543 
   Size:        378     165
 
Bsp1286I G_dGCh'C

Cuts at:      0     404     471     543 
   Size:        404      67      72

  Fragments arranged by size: 

                 404      72      67
 
BsrDI GCAATG_nn'

Cuts at:      0     532     543 
   Size:        532      11
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     441     476     543 
   Size:        441      35      67

  Fragments arranged by size: 

                 441      67      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     122     543 
   Size:        122     421
 
EcoICRI GAG'CTC

Cuts at:      0     402     543 
   Size:        402     141
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     403     470     543 
   Size:        269     134      67      73

  Fragments arranged by size: 

                 269     134      73      67
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     537     543 
   Size:        332     205       6

  Fragments arranged by size: 

                 332     205       6
 
MspA1I CmG'CkG

Cuts at:      0     298     543 
   Size:        298     245
 
NspI r_CATG'y

Cuts at:      0     435     543 
   Size:        435     108
 
PmlI CAC'GTG

Cuts at:      0     455     543 
   Size:        455      88
 
PstI C_TGCA'G

Cuts at:      0     344     543 
   Size:        344     199
 
PvuII CAG'CTG

Cuts at:      0     298     543 
   Size:        298     245
 
SacI G_AGCT'C

Cuts at:      0     404     543 
   Size:        404     139
 
SapI GCTCTTCn'nnn_

Cuts at:      0     122     543 
   Size:        122     421
 
SfcI C'TryA_G

Cuts at:      0     340     543 
   Size:        340     203
 
SmlI C'TyrA_G

Cuts at:      0     403     543 
   Size:        403     140
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      29     543 
   Size:         29     514
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     157     189     543 
   Size:        157      32     354

  Fragments arranged by size: 

                 354     157      32
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     AvaI    BanII     BbsI    BbvCI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI  BsiHKAI
     BsmI Bsp1286I    BsrDI    CspCI     EarI  EcoICRI  Eco57MI     MmeI
   MspA1I     NspI     PmlI     PstI    PvuII     SacI     SapI     SfcI
     SmlI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BsaBI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI