Restriction map for
pF1KB7926
(Linear) (Six-base) MAPSORT of: KIBB7926.seq Check: 926 from: 1 to: 1050
KIBB7926.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 1050
Size: 201 849
AflIII A'CryG_T
Cuts at: 0 858 1050
Size: 858 192
ApoI r'AATT_y
Cuts at: 0 868 1050
Size: 868 182
AseI AT'TA_AT
Cuts at: 0 368 1050
Size: 368 682
BanI G'GyrC_C
Cuts at: 0 135 1050
Size: 135 915
BanII G_rGCy'C
Cuts at: 0 159 1050
Size: 159 891
BbeI G_GCGC'C
Cuts at: 0 139 1050
Size: 139 911
BbsI GAAGACnn'nnnn_
Cuts at: 0 442 1050
Size: 442 608
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 74 1050
Size: 40 34 976
Fragments arranged by size:
976 40 34
BclI T'GATC_A
Cuts at: 0 596 927 1050
Size: 596 331 123
Fragments arranged by size:
596 331 123
BfrBI ATG'CAT
Cuts at: 0 630 1050
Size: 630 420
BglII A'GATC_T
Cuts at: 0 706 1050
Size: 706 344
BmgBI CAC'GTC
Cuts at: 0 970 1050
Size: 970 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 289 343 790 1050
Size: 211 78 54 447 260
Fragments arranged by size:
447 260 211 78 54
Bpu10I CC'TnA_GC
Cuts at: 0 185 1050
Size: 185 865
BsaHI Gr'CG_yC
Cuts at: 0 136 1050
Size: 136 914
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 141 171 1050
Size: 141 30 879
Fragments arranged by size:
879 141 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 259 277 286 298 301 304 307
Size: 139 120 18 9 12 3 3 3
Cuts at: 307 367 370 1025 1050
Size: 60 3 655 25
Fragments arranged by size:
655 139 120 60 25 18 12 9
3 3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1050
Size: 92 958
BsmBI CGTCTCn'nnnn_
Cuts at: 0 142 1050
Size: 142 908
Bsp1286I G_dGCh'C
Cuts at: 0 159 1050
Size: 159 891
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 678 1050
Size: 678 372
BsrDI GCAATG_nn'
Cuts at: 0 995 1050
Size: 995 55
BsrFI r'CCGG_y
Cuts at: 0 132 332 1050
Size: 132 200 718
Fragments arranged by size:
718 200 132
BsrGI T'GTAC_A
Cuts at: 0 584 1050
Size: 584 466
BstYI r'GATC_y
Cuts at: 0 706 1050
Size: 706 344
EarI CTCTTCn'nnn_
Cuts at: 0 254 275 1050
Size: 254 21 775
Fragments arranged by size:
775 254 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 65 1050
Size: 65 985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 211 289 343 790 1050
Size: 201 10 78 54 447 260
Fragments arranged by size:
447 260 201 78 54 10
HaeII r_GCGC'y
Cuts at: 0 41 139 363 1050
Size: 41 98 224 687
Fragments arranged by size:
687 224 98 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 525 557 1050
Size: 141 32 352 32 493
Fragments arranged by size:
493 352 141 32 32
KasI G'GCGC_C
Cuts at: 0 135 1050
Size: 135 915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 539 917 990 1050
Size: 230 309 378 73 60
Fragments arranged by size:
378 309 230 73 60
MspA1I CmG'CkG
Cuts at: 0 118 1050
Size: 118 932
NaeI GCC'GGC
Cuts at: 0 334 1050
Size: 334 716
NarI GG'CG_CC
Cuts at: 0 136 1050
Size: 136 914
NgoMIV G'CCGG_C
Cuts at: 0 332 1050
Size: 332 718
NsiI A_TGCA'T
Cuts at: 0 632 1050
Size: 632 418
NspI r_CATG'y
Cuts at: 0 862 1050
Size: 862 188
PciI A'CATG_T
Cuts at: 0 858 1050
Size: 858 192
PshAI GACnn'nnGTC
Cuts at: 0 302 1050
Size: 302 748
PsiI TTA'TAA
Cuts at: 0 548 1050
Size: 548 502
PstI C_TGCA'G
Cuts at: 0 63 1050
Size: 63 987
SfcI C'TryA_G
Cuts at: 0 59 492 1050
Size: 59 433 558
Fragments arranged by size:
558 433 59
SfoI GGC'GCC
Cuts at: 0 137 1050
Size: 137 913
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 578 1050
Size: 578 472
TatI w'GTAC_w
Cuts at: 0 525 584 1050
Size: 525 59 466
Fragments arranged by size:
525 466 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 671 703 1050
Size: 671 32 347
Fragments arranged by size:
671 347 32
XbaI T'CTAG_A
Cuts at: 0 603 654 1050
Size: 603 51 396
Fragments arranged by size:
603 396 51
XmnI GAAnn'nnTTC
Cuts at: 0 449 1050
Size: 449 601
Enzymes that do cut:
AcuI AflIII ApoI AseI BanI BanII BbeI BbsI
BcgI BclI BfrBI BglII BmgBI BpmI Bpu10I BsaHI
BsaXI BseRI BsgI BsmBI Bsp1286I BspMI BsrDI BsrFI
BsrGI BstYI EarI EciI Eco57MI HaeII Hin4I KasI
MmeI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PshAI PsiI PstI SfcI SfoI TaqII TatI TstI
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI BglI
BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BsrBI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI Tth111I XcmI XhoI
XmaI ZraI