(Linear) (Six-base) MAPSORT of: KIBB7927.seq  Check: 6229  from: 1  to: 600



KIBB7927.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     600 
   Size:         70     530
 
AflIII A'CryG_T

Cuts at:      0     447     600 
   Size:        447     153
 
ApoI r'AATT_y

Cuts at:      0     102     600 
   Size:        102     498
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     542     600 
   Size:        542      58
 
BglII A'GATC_T

Cuts at:      0      87     600 
   Size:         87     513
 
Bme1580I G_kGCm'C

Cuts at:      0     447     600 
   Size:        447     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     479     600 
   Size:        122     357     121

  Fragments arranged by size: 

                 357     122     121
 
BsaWI w'CCGG_w

Cuts at:      0     218     600 
   Size:        218     382
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     452     482     600 
   Size:        452      30     118

  Fragments arranged by size: 

                 452     118      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     134     600 
   Size:         73      61     466

  Fragments arranged by size: 

                 466      73      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     600 
   Size:         37     563
 
BsiHKAI G_wGCw'C

Cuts at:      0     465     600 
   Size:        465     135
 
BsmI GAATG_Cn'

Cuts at:      0     534     600 
   Size:        534      66
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      55     600 
   Size:         55     545
 
Bsp1286I G_dGCh'C

Cuts at:      0     447     465     600 
   Size:        447      18     135

  Fragments arranged by size: 

                 447     135      18
 
BsrDI GCAATG_nn'

Cuts at:      0     314     334     600 
   Size:        314      20     266

  Fragments arranged by size: 

                 314     266      20
 
BstYI r'GATC_y

Cuts at:      0      87     600 
   Size:         87     513
 
Bsu36I CC'TnA_GG

Cuts at:      0     212     600 
   Size:        212     388
 
EaeI y'GGCC_r

Cuts at:      0       2     600 
   Size:          2     598
 
EarI CTCTTCn'nnn_

Cuts at:      0      46     600 
   Size:         46     554
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     122     479     600 
   Size:         70      52     357     121

  Fragments arranged by size: 

                 357     121      70      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0     133     600 
   Size:        133     467
 
EcoRV GAT'ATC

Cuts at:      0     282     600 
   Size:        282     318
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     106     600 
   Size:        106     494
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      57      89     600 
   Size:         57      32     511

  Fragments arranged by size: 

                 511      57      32
 
MscI TGG'CCA

Cuts at:      0       4     600 
   Size:          4     596
 
MslI CAynn'nnrTG

Cuts at:      0      13     452     600 
   Size:         13     439     148

  Fragments arranged by size: 

                 439     148      13
 
MspA1I CmG'CkG

Cuts at:      0     304     355     600 
   Size:        304      51     245

  Fragments arranged by size: 

                 304     245      51
 
NdeI CA'TA_TG

Cuts at:      0     150     600 
   Size:        150     450
 
NspI r_CATG'y

Cuts at:      0     451     600 
   Size:        451     149
 
PciI A'CATG_T

Cuts at:      0     447     600 
   Size:        447     153
 
PpuMI rG'GwC_Cy

Cuts at:      0     133     600 
   Size:        133     467
 
PstI C_TGCA'G

Cuts at:      0     360     600 
   Size:        360     240
 
PvuII CAG'CTG

Cuts at:      0     304     355     600 
   Size:        304      51     245

  Fragments arranged by size: 

                 304     245      51
 
SapI GCTCTTCn'nnn_

Cuts at:      0      46     600 
   Size:         46     554
 
SfcI C'TryA_G

Cuts at:      0     356     383     600 
   Size:        356      27     217

  Fragments arranged by size: 

                 356     217      27
 
SspI AAT'ATT

Cuts at:      0     249     600 
   Size:        249     351
 
TatI w'GTAC_w

Cuts at:      0     398     600 
   Size:        398     202
 
XmnI GAAnn'nnTTC

Cuts at:      0     587     600 
   Size:        587      13
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     BbsI    BglII Bme1580I     BpmI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrDI
    BstYI   Bsu36I     EaeI     EarI  Eco57MI EcoO109I    EcoRV     FalI
    Hin4I     MscI     MslI   MspA1I     NdeI     NspI     PciI    PpuMI
     PstI    PvuII     SapI     SfcI     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BseYI    BsiEI    BsiWI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI