(Linear) (Six-base) MAPSORT of: KIBB7927.seq Check: 6229 from: 1 to: 600 KIBB7927.orf With 182 enzymes: SgfI * October 7, 2009 14:25 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 600 Size: 70 530 AflIII A'CryG_T Cuts at: 0 447 600 Size: 447 153 ApoI r'AATT_y Cuts at: 0 102 600 Size: 102 498 BbsI GAAGACnn'nnnn_ Cuts at: 0 542 600 Size: 542 58 BglII A'GATC_T Cuts at: 0 87 600 Size: 87 513 Bme1580I G_kGCm'C Cuts at: 0 447 600 Size: 447 153 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 479 600 Size: 122 357 121 Fragments arranged by size: 357 122 121 BsaWI w'CCGG_w Cuts at: 0 218 600 Size: 218 382 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 452 482 600 Size: 452 30 118 Fragments arranged by size: 452 118 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 73 134 600 Size: 73 61 466 Fragments arranged by size: 466 73 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 600 Size: 37 563 BsiHKAI G_wGCw'C Cuts at: 0 465 600 Size: 465 135 BsmI GAATG_Cn' Cuts at: 0 534 600 Size: 534 66 BsmBI CGTCTCn'nnnn_ Cuts at: 0 55 600 Size: 55 545 Bsp1286I G_dGCh'C Cuts at: 0 447 465 600 Size: 447 18 135 Fragments arranged by size: 447 135 18 BsrDI GCAATG_nn' Cuts at: 0 314 334 600 Size: 314 20 266 Fragments arranged by size: 314 266 20 BstYI r'GATC_y Cuts at: 0 87 600 Size: 87 513 Bsu36I CC'TnA_GG Cuts at: 0 212 600 Size: 212 388 EaeI y'GGCC_r Cuts at: 0 2 600 Size: 2 598 EarI CTCTTCn'nnn_ Cuts at: 0 46 600 Size: 46 554 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 122 479 600 Size: 70 52 357 121 Fragments arranged by size: 357 121 70 52 EcoO109I rG'GnC_Cy Cuts at: 0 133 600 Size: 133 467 EcoRV GAT'ATC Cuts at: 0 282 600 Size: 282 318 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 106 600 Size: 106 494 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 57 89 600 Size: 57 32 511 Fragments arranged by size: 511 57 32 MscI TGG'CCA Cuts at: 0 4 600 Size: 4 596 MslI CAynn'nnrTG Cuts at: 0 13 452 600 Size: 13 439 148 Fragments arranged by size: 439 148 13 MspA1I CmG'CkG Cuts at: 0 304 355 600 Size: 304 51 245 Fragments arranged by size: 304 245 51 NdeI CA'TA_TG Cuts at: 0 150 600 Size: 150 450 NspI r_CATG'y Cuts at: 0 451 600 Size: 451 149 PciI A'CATG_T Cuts at: 0 447 600 Size: 447 153 PpuMI rG'GwC_Cy Cuts at: 0 133 600 Size: 133 467 PstI C_TGCA'G Cuts at: 0 360 600 Size: 360 240 PvuII CAG'CTG Cuts at: 0 304 355 600 Size: 304 51 245 Fragments arranged by size: 304 245 51 SapI GCTCTTCn'nnn_ Cuts at: 0 46 600 Size: 46 554 SfcI C'TryA_G Cuts at: 0 356 383 600 Size: 356 27 217 Fragments arranged by size: 356 217 27 SspI AAT'ATT Cuts at: 0 249 600 Size: 249 351 TatI w'GTAC_w Cuts at: 0 398 600 Size: 398 202 XmnI GAAnn'nnTTC Cuts at: 0 587 600 Size: 587 13 Enzymes that do cut: AcuI AflIII ApoI BbsI BglII Bme1580I BpmI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BstYI Bsu36I EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I MscI MslI MspA1I NdeI NspI PciI PpuMI PstI PvuII SapI SfcI SspI TatI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI