Restriction map for
pF1KB7928
(Linear) (Six-base) MAPSORT of: KIBB7928.seq Check: 147 from: 1 to: 600
KIBB7928.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 563 600
Size: 445 118 37
Fragments arranged by size:
445 118 37
AvaI C'yCGr_G
Cuts at: 0 117 574 600
Size: 117 457 26
Fragments arranged by size:
457 117 26
BanII G_rGCy'C
Cuts at: 0 86 541 600
Size: 86 455 59
Fragments arranged by size:
455 86 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 600
Size: 496 104
BclI T'GATC_A
Cuts at: 0 251 600
Size: 251 349
Bme1580I G_kGCm'C
Cuts at: 0 575 600
Size: 575 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 474 600
Size: 474 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 535 600
Size: 70 465 65
Fragments arranged by size:
465 70 65
BsaWI w'CCGG_w
Cuts at: 0 325 600
Size: 325 275
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 320 337 350 600
Size: 307 13 17 13 250
Fragments arranged by size:
307 250 17 13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 600
Size: 424 176
BseYI C'CCAG_C
Cuts at: 0 461 600
Size: 461 139
Bsp1286I G_dGCh'C
Cuts at: 0 86 541 575 600
Size: 86 455 34 25
Fragments arranged by size:
455 86 34 25
BspEI T'CCGG_A
Cuts at: 0 325 600
Size: 325 275
BsrBI CCG'CTC
Cuts at: 0 26 600
Size: 26 574
BsrFI r'CCGG_y
Cuts at: 0 225 600
Size: 225 375
BstAPI GCAn_nnn'nTGC
Cuts at: 0 56 600
Size: 56 544
BstXI CCAn_nnnn'nTGG
Cuts at: 0 79 339 375 600
Size: 79 260 36 225
Fragments arranged by size:
260 225 79 36
BstYI r'GATC_y
Cuts at: 0 372 419 434 600
Size: 372 47 15 166
Fragments arranged by size:
372 166 47 15
BtgI C'CryG_G
Cuts at: 0 368 600
Size: 368 232
EaeI y'GGCC_r
Cuts at: 0 341 600
Size: 341 259
EarI CTCTTCn'nnn_
Cuts at: 0 313 539 600
Size: 313 226 61
Fragments arranged by size:
313 226 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 445 535 563 600
Size: 70 375 90 28 37
Fragments arranged by size:
375 90 70 37 28
EcoO109I rG'GnC_Cy
Cuts at: 0 237 579 600
Size: 237 342 21
Fragments arranged by size:
342 237 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 600
Size: 183 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 600
Size: 10 32 558
Fragments arranged by size:
558 32 10
MscI TGG'CCA
Cuts at: 0 343 600
Size: 343 257
MslI CAynn'nnrTG
Cuts at: 0 77 600
Size: 77 523
MspA1I CmG'CkG
Cuts at: 0 101 150 513 600
Size: 101 49 363 87
Fragments arranged by size:
363 101 87 49
NcoI C'CATG_G
Cuts at: 0 368 600
Size: 368 232
PpuMI rG'GwC_Cy
Cuts at: 0 237 600
Size: 237 363
PstI C_TGCA'G
Cuts at: 0 155 600
Size: 155 445
PvuII CAG'CTG
Cuts at: 0 150 513 600
Size: 150 363 87
Fragments arranged by size:
363 150 87
SanDI GG'GwC_CC
Cuts at: 0 237 600
Size: 237 363
SapI GCTCTTCn'nnn_
Cuts at: 0 313 539 600
Size: 313 226 61
Fragments arranged by size:
313 226 61
SfcI C'TryA_G
Cuts at: 0 128 151 600
Size: 128 23 449
Fragments arranged by size:
449 128 23
SmaI CCC'GGG
Cuts at: 0 119 600
Size: 119 481
StyI C'CwwG_G
Cuts at: 0 368 600
Size: 368 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 320 339 352 600
Size: 307 13 19 13 248
Fragments arranged by size:
307 248 19 13 13
XmaI C'CCGG_G
Cuts at: 0 117 600
Size: 117 483
Enzymes that do cut:
AcuI AvaI BanII BbsI BclI Bme1580I BplI BpmI
BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BsrBI BsrFI
BstAPI BstXI BstYI BtgI EaeI EarI Eco57MI EcoO109I
FalI Hin4I MscI MslI MspA1I NcoI PpuMI PstI
PvuII SanDI SapI SfcI SmaI StyI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmnI ZraI