Restriction map for
pF1KB7932
(Linear) (Six-base) MAPSORT of: KIBB7932.seq Check: 9604 from: 1 to: 639
KIBB7932.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 466 639
Size: 466 173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 339 639
Size: 95 244 300
Fragments arranged by size:
300 244 95
BanI G'GyrC_C
Cuts at: 0 100 145 639
Size: 100 45 494
Fragments arranged by size:
494 100 45
BanII G_rGCy'C
Cuts at: 0 212 639
Size: 212 427
BbeI G_GCGC'C
Cuts at: 0 104 149 639
Size: 104 45 490
Fragments arranged by size:
490 104 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 572 639
Size: 86 486 67
Fragments arranged by size:
486 86 67
BglI GCCn_nnn'nGGC
Cuts at: 0 261 639
Size: 261 378
BglII A'GATC_T
Cuts at: 0 558 639
Size: 558 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 30 151 639
Size: 30 121 488
Fragments arranged by size:
488 121 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 613 639
Size: 472 141 26
Fragments arranged by size:
472 141 26
BsaHI Gr'CG_yC
Cuts at: 0 56 101 146 639
Size: 56 45 45 493
Fragments arranged by size:
493 56 45 45
BsaWI w'CCGG_w
Cuts at: 0 497 639
Size: 497 142
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 106 121 166 169 639
Size: 61 45 15 45 3 470
Fragments arranged by size:
470 61 45 45 15 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 639
Size: 601 38
BsiEI CG_ry'CG
Cuts at: 0 175 639
Size: 175 464
Bsp1286I G_dGCh'C
Cuts at: 0 212 639
Size: 212 427
BspEI T'CCGG_A
Cuts at: 0 497 639
Size: 497 142
BsrBI CCG'CTC
Cuts at: 0 597 639
Size: 597 42
BsrDI GCAATG_nn'
Cuts at: 0 614 639
Size: 614 25
BstEII G'GTnAC_C
Cuts at: 0 340 639
Size: 340 299
BstYI r'GATC_y
Cuts at: 0 558 639
Size: 558 81
BstZ17I GTA'TAC
Cuts at: 0 467 639
Size: 467 172
BtgI C'CryG_G
Cuts at: 0 175 639
Size: 175 464
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 495 639
Size: 495 144
BtsI GCAGTG_nn'
Cuts at: 0 454 639
Size: 454 185
EaeI y'GGCC_r
Cuts at: 0 172 639
Size: 172 467
EagI C'GGCC_G
Cuts at: 0 172 639
Size: 172 467
EarI CTCTTCn'nnn_
Cuts at: 0 56 116 358 639
Size: 56 60 242 281
Fragments arranged by size:
281 242 60 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 639
Size: 28 611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 339 472 613 639
Size: 95 244 133 141 26
Fragments arranged by size:
244 141 133 95 26
HaeII r_GCGC'y
Cuts at: 0 104 149 639
Size: 104 45 490
Fragments arranged by size:
490 104 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 521 553 639
Size: 521 32 86
Fragments arranged by size:
521 86 32
HincII GTy'rAC
Cuts at: 0 390 426 639
Size: 390 36 213
Fragments arranged by size:
390 213 36
HpaI GTT'AAC
Cuts at: 0 390 639
Size: 390 249
KasI G'GCGC_C
Cuts at: 0 100 145 639
Size: 100 45 494
Fragments arranged by size:
494 100 45
MslI CAynn'nnrTG
Cuts at: 0 575 639
Size: 575 64
MspA1I CmG'CkG
Cuts at: 0 177 639
Size: 177 462
NarI GG'CG_CC
Cuts at: 0 101 146 639
Size: 101 45 493
Fragments arranged by size:
493 101 45
NotI GC'GGCC_GC
Cuts at: 0 172 639
Size: 172 467
SacII CC_GC'GG
Cuts at: 0 178 639
Size: 178 461
SapI GCTCTTCn'nnn_
Cuts at: 0 358 639
Size: 358 281
SfoI GGC'GCC
Cuts at: 0 102 147 639
Size: 102 45 492
Fragments arranged by size:
492 102 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 587 639
Size: 587 52
Tth111I GACn'n_nGTC
Cuts at: 0 538 639
Size: 538 101
Enzymes that do cut:
AccI AcuI BanI BanII BbeI BbsI BglI BglII
BmrI BpmI BsaHI BsaWI BseRI BsgI BsiEI Bsp1286I
BspEI BsrBI BsrDI BstEII BstYI BstZ17I BtgI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI HaeII Hin4I
HincII HpaI KasI MslI MspA1I NarI NotI SacII
SapI SfoI TaqII Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseYI
BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI Bsu36I ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HindIII KpnI MfeI MluI
MmeI MscI NaeI NcoI NdeI NgoMIV NheI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI