(Linear) (Six-base) MAPSORT of: KIBB7934.seq  Check: 1653  from: 1  to: 645



KIBB7934.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     472     645 
   Size:         86     386     173

  Fragments arranged by size: 

                 386     173      86
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     113     645 
   Size:        113     532
 
ApoI r'AATT_y

Cuts at:      0     175     645 
   Size:        175     470
 
BamHI G'GATC_C

Cuts at:      0     270     645 
   Size:        270     375
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     154     645 
   Size:        154     491
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      49      83     645 
   Size:         49      34     562

  Fragments arranged by size: 

                 562      49      34
 
BfrBI ATG'CAT

Cuts at:      0      49     645 
   Size:         49     596
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     394     645 
   Size:        394     251
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     574     604     607     610     645 
   Size:         88     486      30       3       3      35

  Fragments arranged by size: 

                 486      88      35      30       3       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     136     645 
   Size:        136     509
 
Bsp1286I G_dGCh'C

Cuts at:      0     136     645 
   Size:        136     509
 
BstYI r'GATC_y

Cuts at:      0      12     270     645 
   Size:         12     258     375

  Fragments arranged by size: 

                 375     258      12
 
BtsI GCAGTG_nn'

Cuts at:      0     404     645 
   Size:        404     241
 
EarI CTCTTCn'nnn_

Cuts at:      0     316     551     575     645 
   Size:        316     235      24      70

  Fragments arranged by size: 

                 316     235      70      24
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     472     645 
   Size:         86     386     173

  Fragments arranged by size: 

                 386     173      86
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     242     645 
   Size:        242     403
 
HincII GTy'rAC

Cuts at:      0     108     645 
   Size:        108     537
 
MslI CAynn'nnrTG

Cuts at:      0      95     645 
   Size:         95     550
 
NdeI CA'TA_TG

Cuts at:      0      45     645 
   Size:         45     600
 
NsiI A_TGCA'T

Cuts at:      0      51     645 
   Size:         51     594
 
PstI C_TGCA'G

Cuts at:      0     414     645 
   Size:        414     231
 
SfcI C'TryA_G

Cuts at:      0     410     645 
   Size:        410     235
 
StuI AGG'CCT

Cuts at:      0     291     645 
   Size:        291     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     107     297     645 
   Size:        107     190     348

  Fragments arranged by size: 

                 348     190     107
 
Tth111I GACn'n_nGTC

Cuts at:      0     153     645 
   Size:        153     492
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI    BamHI     BbsI     BcgI    BfrBI     BmrI
    BseRI  BsiHKAI Bsp1286I    BstYI     BtsI     EarI  Eco57MI    EcoNI
   HincII     MslI     NdeI     NsiI     PstI     SfcI     StuI    TaqII
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI