Restriction map for
pF1KB7936
(Linear) (Six-base) MAPSORT of: KIBB7936.seq Check: 9920 from: 1 to: 648
KIBB7936.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AatII G_ACGT'C
Cuts at: 0 530 648
Size: 530 118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 248 648
Size: 248 400
ApoI r'AATT_y
Cuts at: 0 580 648
Size: 580 68
AvaI C'yCGr_G
Cuts at: 0 106 124 648
Size: 106 18 524
Fragments arranged by size:
524 106 18
BanI G'GyrC_C
Cuts at: 0 16 648
Size: 16 632
BbsI GAAGACnn'nnnn_
Cuts at: 0 218 522 648
Size: 218 304 126
Fragments arranged by size:
304 218 126
BciVI GTATCCnnnnn_n'
Cuts at: 0 412 648
Size: 412 236
BglII A'GATC_T
Cuts at: 0 227 648
Size: 227 421
Bme1580I G_kGCm'C
Cuts at: 0 19 193 648
Size: 19 174 455
Fragments arranged by size:
455 174 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 188 648
Size: 188 460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 136 610 648
Size: 100 36 474 38
Fragments arranged by size:
474 100 38 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 626 648
Size: 626 22
BsaAI yAC'GTr
Cuts at: 0 266 648
Size: 266 382
BsaHI Gr'CG_yC
Cuts at: 0 527 648
Size: 527 121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 648
Size: 448 200
BsiHKAI G_wGCw'C
Cuts at: 0 497 648
Size: 497 151
Bsp1286I G_dGCh'C
Cuts at: 0 19 193 497 648
Size: 19 174 304 151
Fragments arranged by size:
304 174 151 19
BspHI T'CATG_A
Cuts at: 0 614 648
Size: 614 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 150 648
Size: 150 498
BsrBI CCG'CTC
Cuts at: 0 340 648
Size: 340 308
BssSI C'ACGA_G
Cuts at: 0 370 648
Size: 370 278
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 648
Size: 201 447
BstYI r'GATC_y
Cuts at: 0 227 648
Size: 227 421
BtgI C'CryG_G
Cuts at: 0 89 648
Size: 89 559
EarI CTCTTCn'nnn_
Cuts at: 0 286 648
Size: 286 362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 136 610 648
Size: 100 36 474 38
Fragments arranged by size:
474 100 38 36
EcoO109I rG'GnC_Cy
Cuts at: 0 162 648
Size: 162 486
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 350 382 648
Size: 219 32 99 32 266
Fragments arranged by size:
266 219 99 32 32
HincII GTy'rAC
Cuts at: 0 207 648
Size: 207 441
MslI CAynn'nnrTG
Cuts at: 0 254 648
Size: 254 394
MspA1I CmG'CkG
Cuts at: 0 630 648
Size: 630 18
NcoI C'CATG_G
Cuts at: 0 89 648
Size: 89 559
NspI r_CATG'y
Cuts at: 0 523 648
Size: 523 125
PflMI CCAn_nnn'nTGG
Cuts at: 0 543 648
Size: 543 105
PpuMI rG'GwC_Cy
Cuts at: 0 162 648
Size: 162 486
PspXI vC'TCGA_Gb
Cuts at: 0 124 648
Size: 124 524
PstI C_TGCA'G
Cuts at: 0 161 648
Size: 161 487
PvuII CAG'CTG
Cuts at: 0 630 648
Size: 630 18
SfcI C'TryA_G
Cuts at: 0 157 648
Size: 157 491
SmlI C'TyrA_G
Cuts at: 0 124 648
Size: 124 524
StyI C'CwwG_G
Cuts at: 0 89 511 648
Size: 89 422 137
Fragments arranged by size:
422 137 89
Tth111I GACn'n_nGTC
Cuts at: 0 448 648
Size: 448 200
XhoI C'TCGA_G
Cuts at: 0 124 648
Size: 124 524
ZraI GAC'GTC
Cuts at: 0 528 648
Size: 528 120
Enzymes that do cut:
AatII AlfI ApoI AvaI BanI BbsI BciVI BglII
Bme1580I BmrI BpmI BsaI BsaAI BsaHI BseRI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BssSI BstXI BstYI BtgI
EarI Eco57MI EcoO109I Hin4I HincII MslI MspA1I NcoI
NspI PflMI PpuMI PspXI PstI PvuII SfcI SmlI
StyI Tth111I XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BclI BfrBI BglI BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaBI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI XbaI XcmI XmaI XmnI