Restriction map for
pF1KB7937
(Linear) (Six-base) MAPSORT of: KIBB7937.seq Check: 6234 from: 1 to: 1128
KIBB7937.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 430 600 912 1128
Size: 430 170 312 216
Fragments arranged by size:
430 312 216 170
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 863 932 1128
Size: 472 391 69 196
Fragments arranged by size:
472 391 196 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 404 1128
Size: 404 724
AlwNI CAG_nnn'CTG
Cuts at: 0 615 1128
Size: 615 513
ApoI r'AATT_y
Cuts at: 0 471 1048 1128
Size: 471 577 80
Fragments arranged by size:
577 471 80
AseI AT'TA_AT
Cuts at: 0 65 1128
Size: 65 1063
BanI G'GyrC_C
Cuts at: 0 511 1128
Size: 511 617
BanII G_rGCy'C
Cuts at: 0 938 1128
Size: 938 190
BbsI GAAGACnn'nnnn_
Cuts at: 0 872 896 1128
Size: 872 24 232
Fragments arranged by size:
872 232 24
BbvCI CC'TCA_GC
Cuts at: 0 275 290 533 668 1128
Size: 275 15 243 135 460
Fragments arranged by size:
460 275 243 135 15
BciVI GTATCCnnnnn_n'
Cuts at: 0 825 1128
Size: 825 303
BfrBI ATG'CAT
Cuts at: 0 391 1128
Size: 391 737
BglII A'GATC_T
Cuts at: 0 43 1128
Size: 43 1085
BlpI GC'TnA_GC
Cuts at: 0 269 1128
Size: 269 859
Bme1580I G_kGCm'C
Cuts at: 0 516 1128
Size: 516 612
BmrI ACTGGGnnnn_n'
Cuts at: 0 836 1128
Size: 836 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1128
Size: 650 478
Bpu10I CC'TnA_GC
Cuts at: 0 275 290 426 533 668 1128
Size: 275 15 136 107 135 460
Fragments arranged by size:
460 275 136 135 107 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1042 1128
Size: 683 359 86
Fragments arranged by size:
683 359 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 783 813 1128
Size: 783 30 315
Fragments arranged by size:
783 315 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 611 898 1128
Size: 611 287 230
Fragments arranged by size:
611 287 230
BseYI C'CCAG_C
Cuts at: 0 227 305 779 1128
Size: 227 78 474 349
Fragments arranged by size:
474 349 227 78
BsmI GAATG_Cn'
Cuts at: 0 393 1128
Size: 393 735
Bsp1286I G_dGCh'C
Cuts at: 0 516 938 1128
Size: 516 422 190
Fragments arranged by size:
516 422 190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 300 430 600 912 1128
Size: 300 130 170 312 216
Fragments arranged by size:
312 300 216 170 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 436 1128
Size: 436 692
BstYI r'GATC_y
Cuts at: 0 43 1128
Size: 43 1085
EaeI y'GGCC_r
Cuts at: 0 759 1128
Size: 759 369
EarI CTCTTCn'nnn_
Cuts at: 0 179 629 926 1080 1128
Size: 179 450 297 154 48
Fragments arranged by size:
450 297 179 154 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 650 863 932 1128
Size: 472 178 213 69 196
Fragments arranged by size:
472 213 196 178 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 823 855 965 997 1128
Size: 273 32 518 32 110 32 131
Fragments arranged by size:
518 273 131 110 32 32 32
MscI TGG'CCA
Cuts at: 0 761 1128
Size: 761 367
MslI CAynn'nnrTG
Cuts at: 0 386 1128
Size: 386 742
MspA1I CmG'CkG
Cuts at: 0 418 1128
Size: 418 710
NdeI CA'TA_TG
Cuts at: 0 383 1128
Size: 383 745
NsiI A_TGCA'T
Cuts at: 0 393 1128
Size: 393 735
PfoI T'CCnGG_A
Cuts at: 0 627 1128
Size: 627 501
PshAI GACnn'nnGTC
Cuts at: 0 652 1128
Size: 652 476
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 816 848 1128
Size: 816 32 280
Fragments arranged by size:
816 280 32
PstI C_TGCA'G
Cuts at: 0 441 1128
Size: 441 687
PvuII CAG'CTG
Cuts at: 0 418 1128
Size: 418 710
SapI GCTCTTCn'nnn_
Cuts at: 0 179 926 1128
Size: 179 747 202
Fragments arranged by size:
747 202 179
SbfI CC_TGCA'GG
Cuts at: 0 441 1128
Size: 441 687
ScaI AGT'ACT
Cuts at: 0 113 1128
Size: 113 1015
SfcI C'TryA_G
Cuts at: 0 413 437 1128
Size: 413 24 691
Fragments arranged by size:
691 413 24
SmlI C'TyrA_G
Cuts at: 0 698 1057 1128
Size: 698 359 71
Fragments arranged by size:
698 359 71
TatI w'GTAC_w
Cuts at: 0 111 588 1128
Size: 111 477 540
Fragments arranged by size:
540 477 111
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI AseI BanI BanII
BbsI BbvCI BciVI BfrBI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsmI Bsp1286I
BspMI BstAPI BstYI EaeI EarI Eco57MI Hin4I MscI
MslI MspA1I NdeI NsiI PfoI PshAI PsrI PstI
PvuII SapI SbfI ScaI SfcI SmlI TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BclI BglI BmgBI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI