Restriction map for
pF1KB7942
(Linear) (Six-base) MAPSORT of: KIBB7942.seq Check: 2169 from: 1 to: 735
KIBB7942.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 122 405 735
Size: 122 283 330
Fragments arranged by size:
330 283 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 735
Size: 706 29
AflIII A'CryG_T
Cuts at: 0 622 735
Size: 622 113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 331 735
Size: 331 404
AvaI C'yCGr_G
Cuts at: 0 63 735
Size: 63 672
BanI G'GyrC_C
Cuts at: 0 720 735
Size: 720 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 380 735
Size: 380 355
Bme1580I G_kGCm'C
Cuts at: 0 20 735
Size: 20 715
BmgBI CAC'GTC
Cuts at: 0 513 735
Size: 513 222
BmtI G_CTAG'C
Cuts at: 0 472 735
Size: 472 263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 145 214 735
Size: 46 99 69 521
Fragments arranged by size:
521 99 69 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 549 735
Size: 549 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 573 603 735
Size: 573 30 132
Fragments arranged by size:
573 132 30
BseYI C'CCAG_C
Cuts at: 0 42 735
Size: 42 693
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 626 735
Size: 433 193 109
Fragments arranged by size:
433 193 109
BsiEI CG_ry'CG
Cuts at: 0 51 735
Size: 51 684
BsmBI CGTCTCn'nnnn_
Cuts at: 0 503 593 735
Size: 503 90 142
Fragments arranged by size:
503 142 90
Bsp1286I G_dGCh'C
Cuts at: 0 20 735
Size: 20 715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 405 735
Size: 122 283 330
Fragments arranged by size:
330 283 122
BsrBI CCG'CTC
Cuts at: 0 30 342 735
Size: 30 312 393
Fragments arranged by size:
393 312 30
BsrDI GCAATG_nn'
Cuts at: 0 668 735
Size: 668 67
BsrFI r'CCGG_y
Cuts at: 0 36 735
Size: 36 699
BtgI C'CryG_G
Cuts at: 0 602 735
Size: 602 133
EaeI y'GGCC_r
Cuts at: 0 48 180 659 735
Size: 48 132 479 76
Fragments arranged by size:
479 132 76 48
EagI C'GGCC_G
Cuts at: 0 48 735
Size: 48 687
EarI CTCTTCn'nnn_
Cuts at: 0 265 429 735
Size: 265 164 306
Fragments arranged by size:
306 265 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 172 735
Size: 172 563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 145 214 706 735
Size: 46 99 69 492 29
Fragments arranged by size:
492 99 69 46 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 735
Size: 400 335
HaeII r_GCGC'y
Cuts at: 0 43 602 735
Size: 43 559 133
Fragments arranged by size:
559 133 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 336 368 556 573 588 605 735
Size: 336 32 188 17 15 17 130
Fragments arranged by size:
336 188 130 32 17 17 15
HincII GTy'rAC
Cuts at: 0 732 735
Size: 732 3
MscI TGG'CCA
Cuts at: 0 661 735
Size: 661 74
MslI CAynn'nnrTG
Cuts at: 0 655 735
Size: 655 80
MspA1I CmG'CkG
Cuts at: 0 215 249 315 735
Size: 215 34 66 420
Fragments arranged by size:
420 215 66 34
NcoI C'CATG_G
Cuts at: 0 602 735
Size: 602 133
NheI G'CTAG_C
Cuts at: 0 468 735
Size: 468 267
NotI GC'GGCC_GC
Cuts at: 0 48 735
Size: 48 687
NspI r_CATG'y
Cuts at: 0 626 735
Size: 626 109
PciI A'CATG_T
Cuts at: 0 622 735
Size: 622 113
PstI C_TGCA'G
Cuts at: 0 86 320 735
Size: 86 234 415
Fragments arranged by size:
415 234 86
PvuII CAG'CTG
Cuts at: 0 249 315 735
Size: 249 66 420
Fragments arranged by size:
420 249 66
SapI GCTCTTCn'nnn_
Cuts at: 0 429 735
Size: 429 306
SbfI CC_TGCA'GG
Cuts at: 0 86 735
Size: 86 649
SexAI A'CCwGG_T
Cuts at: 0 716 735
Size: 716 19
SfcI C'TryA_G
Cuts at: 0 82 316 735
Size: 82 234 419
Fragments arranged by size:
419 234 82
SmaI CCC'GGG
Cuts at: 0 65 735
Size: 65 670
SrfI GCCC'GGGC
Cuts at: 0 65 735
Size: 65 670
StyI C'CwwG_G
Cuts at: 0 602 692 735
Size: 602 90 43
Fragments arranged by size:
602 90 43
Tth111I GACn'n_nGTC
Cuts at: 0 585 735
Size: 585 150
XmaI C'CCGG_G
Cuts at: 0 63 735
Size: 63 672
Enzymes that do cut:
AarI AcuI AflIII AlfI AvaI BanI BbsI Bme1580I
BmgBI BmtI BpmI BsaI BsaXI BseYI BsgI BsiEI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BtgI EaeI
EagI EarI EciI Eco57MI EcoNI HaeII Hin4I HincII
MscI MslI MspA1I NcoI NheI NotI NspI PciI
PstI PvuII SapI SbfI SexAI SfcI SmaI SrfI
StyI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmrI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiHKAI
BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI ScaI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI TaqII TatI TstI XbaI XcmI XhoI XmnI
ZraI