Restriction map for
pF1KB7943
(Linear) (Six-base) MAPSORT of: KIBB7943.seq Check: 5605 from: 1 to: 1131
KIBB7943.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 321 1131
Size: 321 810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 1131
Size: 101 1030
AleI CACnn'nnGTG
Cuts at: 0 709 980 1131
Size: 709 271 151
Fragments arranged by size:
709 271 151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 328 1131
Size: 328 803
AlwNI CAG_nnn'CTG
Cuts at: 0 604 954 967 1131
Size: 604 350 13 164
Fragments arranged by size:
604 350 164 13
ApaI G_GGCC'C
Cuts at: 0 739 1131
Size: 739 392
ApaLI G'TGCA_C
Cuts at: 0 303 826 1131
Size: 303 523 305
Fragments arranged by size:
523 305 303
AvaI C'yCGr_G
Cuts at: 0 232 323 562 1131
Size: 232 91 239 569
Fragments arranged by size:
569 239 232 91
BanII G_rGCy'C
Cuts at: 0 424 631 739 925 1131
Size: 424 207 108 186 206
Fragments arranged by size:
424 207 206 186 108
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 1131
Size: 265 866
BbvCI CC'TCA_GC
Cuts at: 0 416 605 1131
Size: 416 189 526
Fragments arranged by size:
526 416 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 493 527 1131
Size: 493 34 604
Fragments arranged by size:
604 493 34
BglI GCCn_nnn'nGGC
Cuts at: 0 88 734 1131
Size: 88 646 397
Fragments arranged by size:
646 397 88
BlpI GC'TnA_GC
Cuts at: 0 140 224 515 766 1131
Size: 140 84 291 251 365
Fragments arranged by size:
365 291 251 140 84
Bme1580I G_kGCm'C
Cuts at: 0 307 739 830 893 1131
Size: 307 432 91 63 238
Fragments arranged by size:
432 307 238 91 63
BmgBI CAC'GTC
Cuts at: 0 490 1131
Size: 490 641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 440 1131
Size: 382 58 691
Fragments arranged by size:
691 382 58
Bpu10I CC'TnA_GC
Cuts at: 0 416 605 1131
Size: 416 189 526
Fragments arranged by size:
526 416 189
BsaI GGTCTCn'nnnn_
Cuts at: 0 998 1131
Size: 998 133
BsaHI Gr'CG_yC
Cuts at: 0 902 1131
Size: 902 229
BsaWI w'CCGG_w
Cuts at: 0 362 1131
Size: 362 769
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 690 720 1087 1117 1131
Size: 690 30 367 30 14
Fragments arranged by size:
690 367 30 30 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 986 1131
Size: 598 388 145
Fragments arranged by size:
598 388 145
BseYI C'CCAG_C
Cuts at: 0 83 92 182 464 651 918 1014 1131
Size: 83 9 90 282 187 267 96 117
Fragments arranged by size:
282 267 187 117 96 90 83 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 52 349 913 992 1131
Size: 16 36 297 564 79 139
Fragments arranged by size:
564 297 139 79 36 16
BsiHKAI G_wGCw'C
Cuts at: 0 307 830 1131
Size: 307 523 301
Fragments arranged by size:
523 307 301
Bsp1286I G_dGCh'C
Cuts at: 0 307 424 631 739 830 893 925 1131
Size: 307 117 207 108 91 63 32 206
Fragments arranged by size:
307 207 206 117 108 91 63 32
BspEI T'CCGG_A
Cuts at: 0 362 1131
Size: 362 769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 320 321 1082 1131
Size: 74 246 1 761 49
Fragments arranged by size:
761 246 74 49 1
BsrFI r'CCGG_y
Cuts at: 0 110 1131
Size: 110 1021
BsrGI T'GTAC_A
Cuts at: 0 59 1131
Size: 59 1072
BstAPI GCAn_nnn'nTGC
Cuts at: 0 967 1131
Size: 967 164
BstBI TT'CG_AA
Cuts at: 0 842 1131
Size: 842 289
BstXI CCAn_nnnn'nTGG
Cuts at: 0 822 1131
Size: 822 309
Bsu36I CC'TnA_GG
Cuts at: 0 317 1131
Size: 317 814
BtgI C'CryG_G
Cuts at: 0 426 1131
Size: 426 705
BtsI GCAGTG_nn'
Cuts at: 0 28 925 930 1131
Size: 28 897 5 201
Fragments arranged by size:
897 201 28 5
EaeI y'GGCC_r
Cuts at: 0 296 809 1131
Size: 296 513 322
Fragments arranged by size:
513 322 296
EarI CTCTTCn'nnn_
Cuts at: 0 619 778 1131
Size: 619 159 353
Fragments arranged by size:
619 353 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 784 1131
Size: 784 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 382 440 1131
Size: 101 281 58 691
Fragments arranged by size:
691 281 101 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 385 1131
Size: 316 69 746
Fragments arranged by size:
746 316 69
EcoO109I rG'GnC_Cy
Cuts at: 0 237 447 588 609 736 1131
Size: 237 210 141 21 127 395
Fragments arranged by size:
395 237 210 141 127 21
HaeII r_GCGC'y
Cuts at: 0 56 147 1131
Size: 56 91 984
Fragments arranged by size:
984 91 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 690 722 1131
Size: 690 32 409
Fragments arranged by size:
690 409 32
HincII GTy'rAC
Cuts at: 0 906 1131
Size: 906 225
MscI TGG'CCA
Cuts at: 0 298 1131
Size: 298 833
MslI CAynn'nnrTG
Cuts at: 0 709 980 1131
Size: 709 271 151
Fragments arranged by size:
709 271 151
MspA1I CmG'CkG
Cuts at: 0 468 765 874 918 967 1131
Size: 468 297 109 44 49 164
Fragments arranged by size:
468 297 164 109 49 44
NcoI C'CATG_G
Cuts at: 0 426 1131
Size: 426 705
NspI r_CATG'y
Cuts at: 0 100 127 1131
Size: 100 27 1004
Fragments arranged by size:
1004 100 27
PasI CC'CwG_GG
Cuts at: 0 593 631 718 1107 1131
Size: 593 38 87 389 24
Fragments arranged by size:
593 389 87 38 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 724 804 1131
Size: 724 80 327
Fragments arranged by size:
724 327 80
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 1131
Size: 10 32 1089
Fragments arranged by size:
1089 32 10
PpuMI rG'GwC_Cy
Cuts at: 0 588 609 1131
Size: 588 21 522
Fragments arranged by size:
588 522 21
PspOMI G'GGCC_C
Cuts at: 0 735 1131
Size: 735 396
PspXI vC'TCGA_Gb
Cuts at: 0 232 1131
Size: 232 899
PstI C_TGCA'G
Cuts at: 0 386 785 873 1131
Size: 386 399 88 258
Fragments arranged by size:
399 386 258 88
PvuII CAG'CTG
Cuts at: 0 468 1131
Size: 468 663
RsrII CG'GwC_CG
Cuts at: 0 576 1131
Size: 576 555
SapI GCTCTTCn'nnn_
Cuts at: 0 619 1131
Size: 619 512
SexAI A'CCwGG_T
Cuts at: 0 821 1131
Size: 821 310
SfcI C'TryA_G
Cuts at: 0 382 781 869 1131
Size: 382 399 88 262
Fragments arranged by size:
399 382 262 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 734 1131
Size: 734 397
SmaI CCC'GGG
Cuts at: 0 325 1131
Size: 325 806
SmlI C'TyrA_G
Cuts at: 0 232 1131
Size: 232 899
SphI G_CATG'C
Cuts at: 0 100 127 1131
Size: 100 27 1004
Fragments arranged by size:
1004 100 27
StuI AGG'CCT
Cuts at: 0 1022 1131
Size: 1022 109
StyI C'CwwG_G
Cuts at: 0 426 674 1131
Size: 426 248 457
Fragments arranged by size:
457 426 248
TatI w'GTAC_w
Cuts at: 0 59 1131
Size: 59 1072
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 820 852 922 954 1087 1119
Size: 736 32 52 32 70 32 133 32
Cuts at: 1119 1131
Size: 12
Fragments arranged by size:
736 133 70 52 32 32 32 32
12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 708 819 1131
Size: 708 111 312
Fragments arranged by size:
708 312 111
XhoI C'TCGA_G
Cuts at: 0 232 1131
Size: 232 899
XmaI C'CCGG_G
Cuts at: 0 323 1131
Size: 323 808
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI ApaLI AvaI
BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrFI BsrGI BstAPI
BstBI BstXI Bsu36I BtgI BtsI EaeI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MscI MslI
MspA1I NcoI NspI PasI PflMI PpiI PpuMI PspOMI
PspXI PstI PvuII RsrII SapI SexAI SfcI SfiI
SmaI SmlI SphI StuI StyI TatI TstI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BciVI BclI BfrBI BglII BmrI
BmtI BplI BpuEI BsaAI BsaBI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrDI BssHII BssSI BstEII BstYI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PshAI PsiI PsrI PvuI SacI
SacII SalI SanDI SbfI ScaI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI
XmnI ZraI