Restriction map for
pF1KB7945
(Linear) (Six-base) MAPSORT of: KIBB7945.seq Check: 5599 from: 1 to: 1173
KIBB7945.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 486 1152 1173
Size: 486 666 21
Fragments arranged by size:
666 486 21
Acc65I G'GTAC_C
Cuts at: 0 941 1173
Size: 941 232
AfeI AGC'GCT
Cuts at: 0 1119 1173
Size: 1119 54
AflIII A'CryG_T
Cuts at: 0 951 1173
Size: 951 222
AlwNI CAG_nnn'CTG
Cuts at: 0 152 699 1173
Size: 152 547 474
Fragments arranged by size:
547 474 152
ApoI r'AATT_y
Cuts at: 0 200 1173
Size: 200 973
AvaI C'yCGr_G
Cuts at: 0 772 937 1086 1127 1173
Size: 772 165 149 41 46
Fragments arranged by size:
772 165 149 46 41
BamHI G'GATC_C
Cuts at: 0 253 1173
Size: 253 920
BanI G'GyrC_C
Cuts at: 0 621 941 1005 1173
Size: 621 320 64 168
Fragments arranged by size:
621 320 168 64
BanII G_rGCy'C
Cuts at: 0 764 1173
Size: 764 409
BglI GCCn_nnn'nGGC
Cuts at: 0 808 841 1173
Size: 808 33 332
Fragments arranged by size:
808 332 33
BmgBI CAC'GTC
Cuts at: 0 563 954 1173
Size: 563 391 219
Fragments arranged by size:
563 391 219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 673 1173
Size: 673 500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 721 1092 1173
Size: 147 574 371 81
Fragments arranged by size:
574 371 147 81
BsaWI w'CCGG_w
Cuts at: 0 416 618 659 1173
Size: 416 202 41 514
Fragments arranged by size:
514 416 202 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 80 110 1051 1081 1173
Size: 80 30 941 30 92
Fragments arranged by size:
941 92 80 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 17 68 1145 1173
Size: 17 51 1077 28
Fragments arranged by size:
1077 51 28 17
BseYI C'CCAG_C
Cuts at: 0 453 568 1173
Size: 453 115 605
Fragments arranged by size:
605 453 115
BsiEI CG_ry'CG
Cuts at: 0 524 1173
Size: 524 649
BsiHKAI G_wGCw'C
Cuts at: 0 514 928 1173
Size: 514 414 245
Fragments arranged by size:
514 414 245
BsmBI CGTCTCn'nnnn_
Cuts at: 0 590 1173
Size: 590 583
Bsp1286I G_dGCh'C
Cuts at: 0 514 764 928 1173
Size: 514 250 164 245
Fragments arranged by size:
514 250 245 164
BspEI T'CCGG_A
Cuts at: 0 416 659 1173
Size: 416 243 514
Fragments arranged by size:
514 416 243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 200 486 1152 1173
Size: 200 286 666 21
Fragments arranged by size:
666 286 200 21
BsrBI CCG'CTC
Cuts at: 0 363 1173
Size: 363 810
BsrFI r'CCGG_y
Cuts at: 0 347 523 1173
Size: 347 176 650
Fragments arranged by size:
650 347 176
BstXI CCAn_nnnn'nTGG
Cuts at: 0 285 1173
Size: 285 888
BstYI r'GATC_y
Cuts at: 0 253 1173
Size: 253 920
BtgI C'CryG_G
Cuts at: 0 183 797 1173
Size: 183 614 376
Fragments arranged by size:
614 376 183
BtsI GCAGTG_nn'
Cuts at: 0 879 1154 1173
Size: 879 275 19
Fragments arranged by size:
879 275 19
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 309 344 1173
Size: 309 35 829
Fragments arranged by size:
829 309 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 498 1173
Size: 498 675
EaeI y'GGCC_r
Cuts at: 0 91 521 630 842 1173
Size: 91 430 109 212 331
Fragments arranged by size:
430 331 212 109 91
EagI C'GGCC_G
Cuts at: 0 521 1173
Size: 521 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 100 1173
Size: 100 1073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 721 1092 1173
Size: 147 574 371 81
Fragments arranged by size:
574 371 147 81
EcoO109I rG'GnC_Cy
Cuts at: 0 149 1173
Size: 149 1024
HaeII r_GCGC'y
Cuts at: 0 575 1121 1173
Size: 575 546 52
Fragments arranged by size:
575 546 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 80 112 1173
Size: 80 32 1061
Fragments arranged by size:
1061 80 32
KpnI G_GTAC'C
Cuts at: 0 945 1173
Size: 945 228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 1173
Size: 46 1127
MscI TGG'CCA
Cuts at: 0 844 1173
Size: 844 329
MslI CAynn'nnrTG
Cuts at: 0 140 188 1173
Size: 140 48 985
Fragments arranged by size:
985 140 48
MspA1I CmG'CkG
Cuts at: 0 545 699 964 1173
Size: 545 154 265 209
Fragments arranged by size:
545 265 209 154
NaeI GCC'GGC
Cuts at: 0 525 1173
Size: 525 648
NcoI C'CATG_G
Cuts at: 0 183 797 1173
Size: 183 614 376
Fragments arranged by size:
614 376 183
NgoMIV G'CCGG_C
Cuts at: 0 523 1173
Size: 523 650
PfoI T'CCnGG_A
Cuts at: 0 98 1024 1069 1173
Size: 98 926 45 104
Fragments arranged by size:
926 104 98 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 1173
Size: 619 32 522
Fragments arranged by size:
619 522 32
PstI C_TGCA'G
Cuts at: 0 1149 1173
Size: 1149 24
PvuII CAG'CTG
Cuts at: 0 699 1173
Size: 699 474
SfcI C'TryA_G
Cuts at: 0 1035 1145 1173
Size: 1035 110 28
Fragments arranged by size:
1035 110 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 841 1173
Size: 841 332
SmaI CCC'GGG
Cuts at: 0 939 1088 1173
Size: 939 149 85
Fragments arranged by size:
939 149 85
StyI C'CwwG_G
Cuts at: 0 183 797 1173
Size: 183 614 376
Fragments arranged by size:
614 376 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 291 453 1173
Size: 291 162 720
Fragments arranged by size:
720 291 162
TatI w'GTAC_w
Cuts at: 0 426 1173
Size: 426 747
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 804 1173
Size: 279 525 369
Fragments arranged by size:
525 369 279
XmaI C'CCGG_G
Cuts at: 0 937 1086 1173
Size: 937 149 87
Fragments arranged by size:
937 149 87
XmnI GAAnn'nnTTC
Cuts at: 0 605 1173
Size: 605 568
Enzymes that do cut:
AarI Acc65I AfeI AflIII AlwNI ApoI AvaI BamHI
BanI BanII BglI BmgBI BplI BpmI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BstXI BstYI BtgI BtsI CspCI DrdI
EaeI EagI EciI Eco57MI EcoO109I HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PfoI
PpiI PstI PvuII SfcI SfiI SmaI StyI TaqII
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsgI BsiWI BsmI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DraIII EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI NarI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI ZraI