(Linear) (Six-base) MAPSORT of: KIBB7947.seq  Check: 8230  from: 1  to: 762



KIBB7947.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      20      49     762 
   Size:         20      29     713

  Fragments arranged by size: 

                 713      29      20
 
BamHI G'GATC_C

Cuts at:      0       3     762 
   Size:          3     759
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     577     762 
   Size:         19     558     185

  Fragments arranged by size: 

                 558     185      19
 
BsaHI Gr'CG_yC

Cuts at:      0     299     762 
   Size:        299     463
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     550     580     732     762 
   Size:        550      30     152      30

  Fragments arranged by size: 

                 550     152      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      42     244     762 
   Size:         42     202     518

  Fragments arranged by size: 

                 518     202      42
 
BseYI C'CCAG_C

Cuts at:      0      42     762 
   Size:         42     720
 
BsiHKAI G_wGCw'C

Cuts at:      0     236     418     762 
   Size:        236     182     344

  Fragments arranged by size: 

                 344     236     182
 
Bsp1286I G_dGCh'C

Cuts at:      0     236     418     762 
   Size:        236     182     344

  Fragments arranged by size: 

                 344     236     182
 
BsrDI GCAATG_nn'

Cuts at:      0      93     762 
   Size:         93     669
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     568     762 
   Size:        568     194
 
BstBI TT'CG_AA

Cuts at:      0      66     762 
   Size:         66     696
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     345     360     762 
   Size:        345      15     402

  Fragments arranged by size: 

                 402     345      15
 
BstYI r'GATC_y

Cuts at:      0       3     762 
   Size:          3     759
 
Bsu36I CC'TnA_GG

Cuts at:      0      96     725     762 
   Size:         96     629      37

  Fragments arranged by size: 

                 629      96      37
 
BtgI C'CryG_G

Cuts at:      0       8     762 
   Size:          8     754
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     407     762 
   Size:        407     355
 
BtsI GCAGTG_nn'

Cuts at:      0     416     762 
   Size:        416     346
 
EaeI y'GGCC_r

Cuts at:      0      11     762 
   Size:         11     751
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     465     762 
   Size:        465     297
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     577     762 
   Size:         19     558     185

  Fragments arranged by size: 

                 558     185      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     223     762 
   Size:        223     539
 
EcoO109I rG'GnC_Cy

Cuts at:      0      92     720     762 
   Size:         92     628      42

  Fragments arranged by size: 

                 628      92      42
 
HaeII r_GCGC'y

Cuts at:      0     639     762 
   Size:        639     123
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     424     456     721     753     762 
   Size:        424      32     265      32       9

  Fragments arranged by size: 

                 424     265      32      32       9
 
MspA1I CmG'CkG

Cuts at:      0      10      20     546     762 
   Size:         10      10     526     216

  Fragments arranged by size: 

                 526     216      10      10
 
PpuMI rG'GwC_Cy

Cuts at:      0     720     762 
   Size:        720      42
 
PvuII CAG'CTG

Cuts at:      0      20     546     762 
   Size:         20     526     216

  Fragments arranged by size: 

                 526     216      20
 
SacII CC_GC'GG

Cuts at:      0      11     762 
   Size:         11     751
 
StyI C'CwwG_G

Cuts at:      0     338     536     762 
   Size:        338     198     226

  Fragments arranged by size: 

                 338     226     198
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     732     762 
   Size:        732      30
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      50     762 
   Size:         50     712
 

 Enzymes that do cut:

    AlwNI    BamHI     BpmI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BsrDI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     EaeI     EciI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I   MspA1I    PpuMI    PvuII    SacII     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstEII  BstZ17I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI