Restriction map for
pF1KB7951
(Linear) (Six-base) MAPSORT of: KIBB7951.seq Check: 7599 from: 1 to: 765
KIBB7951.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
Acc65I G'GTAC_C
Cuts at: 0 719 765
Size: 719 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 765
Size: 710 55
AleI CACnn'nnGTG
Cuts at: 0 337 765
Size: 337 428
ApaI G_GGCC'C
Cuts at: 0 612 765
Size: 612 153
AvaI C'yCGr_G
Cuts at: 0 270 765
Size: 270 495
BanI G'GyrC_C
Cuts at: 0 163 719 765
Size: 163 556 46
Fragments arranged by size:
556 163 46
BanII G_rGCy'C
Cuts at: 0 482 612 676 694 765
Size: 482 130 64 18 71
Fragments arranged by size:
482 130 71 64 18
BbeI G_GCGC'C
Cuts at: 0 167 765
Size: 167 598
BglII A'GATC_T
Cuts at: 0 518 765
Size: 518 247
BlpI GC'TnA_GC
Cuts at: 0 174 765
Size: 174 591
Bme1580I G_kGCm'C
Cuts at: 0 116 612 765
Size: 116 496 153
Fragments arranged by size:
496 153 116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 601 765
Size: 322 279 164
Fragments arranged by size:
322 279 164
Bpu10I CC'TnA_GC
Cuts at: 0 675 765
Size: 675 90
BsaHI Gr'CG_yC
Cuts at: 0 164 765
Size: 164 601
BsaWI w'CCGG_w
Cuts at: 0 431 765
Size: 431 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 765
Size: 676 30 59
Fragments arranged by size:
676 59 30
BseYI C'CCAG_C
Cuts at: 0 198 703 765
Size: 198 505 62
Fragments arranged by size:
505 198 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 765
Size: 280 485
BsiEI CG_ry'CG
Cuts at: 0 196 606 765
Size: 196 410 159
Fragments arranged by size:
410 196 159
BsiHKAI G_wGCw'C
Cuts at: 0 334 482 765
Size: 334 148 283
Fragments arranged by size:
334 283 148
Bsp1286I G_dGCh'C
Cuts at: 0 116 334 482 612 676 694 765
Size: 116 218 148 130 64 18 71
Fragments arranged by size:
218 148 130 116 71 64 18
BstYI r'GATC_y
Cuts at: 0 380 518 765
Size: 380 138 247
Fragments arranged by size:
380 247 138
BtgI C'CryG_G
Cuts at: 0 108 765
Size: 108 657
DraIII CAC_nnn'GTG
Cuts at: 0 716 765
Size: 716 49
EaeI y'GGCC_r
Cuts at: 0 596 765
Size: 596 169
EarI CTCTTCn'nnn_
Cuts at: 0 322 663 765
Size: 322 341 102
Fragments arranged by size:
341 322 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 490 765
Size: 331 159 275
Fragments arranged by size:
331 275 159
EcoICRI GAG'CTC
Cuts at: 0 480 765
Size: 480 285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 601 710 765
Size: 322 279 109 55
Fragments arranged by size:
322 279 109 55
EcoO109I rG'GnC_Cy
Cuts at: 0 306 609 765
Size: 306 303 156
Fragments arranged by size:
306 303 156
HaeII r_GCGC'y
Cuts at: 0 167 281 765
Size: 167 114 484
Fragments arranged by size:
484 167 114
HincII GTy'rAC
Cuts at: 0 216 765
Size: 216 549
KasI G'GCGC_C
Cuts at: 0 163 765
Size: 163 602
KpnI G_GTAC'C
Cuts at: 0 723 765
Size: 723 42
MscI TGG'CCA
Cuts at: 0 598 765
Size: 598 167
MslI CAynn'nnrTG
Cuts at: 0 337 765
Size: 337 428
MspA1I CmG'CkG
Cuts at: 0 198 765
Size: 198 567
NarI GG'CG_CC
Cuts at: 0 164 765
Size: 164 601
PasI CC'CwG_GG
Cuts at: 0 310 765
Size: 310 455
PfoI T'CCnGG_A
Cuts at: 0 664 765
Size: 664 101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 640 672 765
Size: 640 32 93
Fragments arranged by size:
640 93 32
PpuMI rG'GwC_Cy
Cuts at: 0 306 765
Size: 306 459
PshAI GACnn'nnGTC
Cuts at: 0 511 765
Size: 511 254
PspOMI G'GGCC_C
Cuts at: 0 608 765
Size: 608 157
SacI G_AGCT'C
Cuts at: 0 482 765
Size: 482 283
SapI GCTCTTCn'nnn_
Cuts at: 0 322 765
Size: 322 443
SfcI C'TryA_G
Cuts at: 0 168 765
Size: 168 597
SfoI GGC'GCC
Cuts at: 0 165 765
Size: 165 600
SmaI CCC'GGG
Cuts at: 0 272 765
Size: 272 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 210 765
Size: 210 555
XmaI C'CCGG_G
Cuts at: 0 270 765
Size: 270 495
Enzymes that do cut:
Acc65I AcuI AleI ApaI AvaI BanI BanII BbeI
BglII BlpI Bme1580I BpmI Bpu10I BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BstYI BtgI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII HincII
KasI KpnI MscI MslI MspA1I NarI PasI PfoI
PpiI PpuMI PshAI PspOMI SacI SapI SfcI SfoI
SmaI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BseRI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HindIII HpaI MfeI
MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI