Restriction map for
pF1KB7952
(Linear) (Six-base) MAPSORT of: KIBB7952.seq Check: 2046 from: 1 to: 1182
KIBB7952.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
Acc65I G'GTAC_C
Cuts at: 0 926 1182
Size: 926 256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 855 939 1182
Size: 481 374 84 243
Fragments arranged by size:
481 374 243 84
AflIII A'CryG_T
Cuts at: 0 249 526 1182
Size: 249 277 656
Fragments arranged by size:
656 277 249
AhdI GACnn_n'nnGTC
Cuts at: 0 445 1182
Size: 445 737
AleI CACnn'nnGTG
Cuts at: 0 764 1182
Size: 764 418
AlwNI CAG_nnn'CTG
Cuts at: 0 1171 1182
Size: 1171 11
ApoI r'AATT_y
Cuts at: 0 821 1182
Size: 821 361
AvaI C'yCGr_G
Cuts at: 0 46 1182
Size: 46 1136
BamHI G'GATC_C
Cuts at: 0 681 1182
Size: 681 501
BanI G'GyrC_C
Cuts at: 0 465 926 1182
Size: 465 461 256
Fragments arranged by size:
465 461 256
BanII G_rGCy'C
Cuts at: 0 323 401 799 1182
Size: 323 78 398 383
Fragments arranged by size:
398 383 323 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 945 1182
Size: 945 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 155 189 1182
Size: 155 34 993
Fragments arranged by size:
993 155 34
BlpI GC'TnA_GC
Cuts at: 0 1172 1182
Size: 1172 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 806 1182
Size: 806 376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 307 310 400 409 851 1182
Size: 202 105 3 90 9 442 331
Fragments arranged by size:
442 331 202 105 90 9 3
Bpu10I CC'TnA_GC
Cuts at: 0 604 798 1182
Size: 604 194 384
Fragments arranged by size:
604 384 194
BsaI GGTCTCn'nnnn_
Cuts at: 0 178 808 1182
Size: 178 630 374
Fragments arranged by size:
630 374 178
BseYI C'CCAG_C
Cuts at: 0 329 503 1182
Size: 329 174 679
Fragments arranged by size:
679 329 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1047 1182
Size: 1038 9 135
Fragments arranged by size:
1038 135 9
BsiHKAI G_wGCw'C
Cuts at: 0 259 323 401 799 1182
Size: 259 64 78 398 383
Fragments arranged by size:
398 383 259 78 64
Bsp1286I G_dGCh'C
Cuts at: 0 259 323 401 799 1182
Size: 259 64 78 398 383
Fragments arranged by size:
398 383 259 78 64
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1025 1182
Size: 1025 157
BstXI CCAn_nnnn'nTGG
Cuts at: 0 556 1182
Size: 556 626
BstYI r'GATC_y
Cuts at: 0 276 384 681 1157 1182
Size: 276 108 297 476 25
Fragments arranged by size:
476 297 276 108 25
Bsu36I CC'TnA_GG
Cuts at: 0 509 1182
Size: 509 673
BtsI GCAGTG_nn'
Cuts at: 0 736 930 1131 1182
Size: 736 194 201 51
Fragments arranged by size:
736 201 194 51
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 59 94 924 959 1182
Size: 59 35 830 35 223
Fragments arranged by size:
830 223 59 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1167 1182
Size: 1167 15
DrdI GACnn_nn'nnGTC
Cuts at: 0 498 1182
Size: 498 684
EarI CTCTTCn'nnn_
Cuts at: 0 356 775 1182
Size: 356 419 407
Fragments arranged by size:
419 407 356
EcoICRI GAG'CTC
Cuts at: 0 321 399 797 1182
Size: 321 78 398 385
Fragments arranged by size:
398 385 321 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 307 310 400 409 481 851 855
Size: 202 105 3 90 9 72 370 4
Cuts at: 855 939 1182
Size: 84 243
Fragments arranged by size:
370 243 202 105 90 84 72 9
4 3
EcoRI G'AATT_C
Cuts at: 0 821 1182
Size: 821 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 261 293 431 453 463 485
Size: 10 32 219 32 138 22 10 22
Cuts at: 485 786 818 954 986 1026 1058 1182
Size: 301 32 136 32 40 32 124
Fragments arranged by size:
301 219 138 136 124 40 32 32
32 32 32 22 22 10 10
HindIII A'AGCT_T
Cuts at: 0 1119 1182
Size: 1119 63
KpnI G_GTAC'C
Cuts at: 0 930 1182
Size: 930 252
MslI CAynn'nnrTG
Cuts at: 0 764 1182
Size: 764 418
MspA1I CmG'CkG
Cuts at: 0 171 1182
Size: 171 1011
NspI r_CATG'y
Cuts at: 0 253 530 1182
Size: 253 277 652
Fragments arranged by size:
652 277 253
PasI CC'CwG_GG
Cuts at: 0 30 1182
Size: 30 1152
PciI A'CATG_T
Cuts at: 0 249 526 1182
Size: 249 277 656
Fragments arranged by size:
656 277 249
PflMI CCAn_nnn'nTGG
Cuts at: 0 355 1167 1182
Size: 355 812 15
Fragments arranged by size:
812 355 15
PfoI T'CCnGG_A
Cuts at: 0 218 1182
Size: 218 964
PvuII CAG'CTG
Cuts at: 0 171 1182
Size: 171 1011
SacI G_AGCT'C
Cuts at: 0 323 401 799 1182
Size: 323 78 398 383
Fragments arranged by size:
398 383 323 78
SapI GCTCTTCn'nnn_
Cuts at: 0 775 1182
Size: 775 407
SfcI C'TryA_G
Cuts at: 0 1038 1182
Size: 1038 144
StyI C'CwwG_G
Cuts at: 0 196 314 1182
Size: 196 118 868
Fragments arranged by size:
868 196 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 321 1182
Size: 22 299 861
Fragments arranged by size:
861 299 22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 187 1182
Size: 187 995
XmnI GAAnn'nnTTC
Cuts at: 0 62 868 952 1182
Size: 62 806 84 230
Fragments arranged by size:
806 230 84 62
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AlwNI ApoI AvaI
BamHI BanI BanII BbsI BcgI BlpI BplI BpmI
Bpu10I BsaI BseYI BsgI BsiHKAI Bsp1286I BstAPI BstXI
BstYI Bsu36I BtsI CspCI DraIII DrdI EarI EcoICRI
Eco57MI EcoRI Hin4I HindIII KpnI MslI MspA1I NspI
PasI PciI PflMI PfoI PvuII SacI SapI SfcI
StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgI BtgZI ClaI DraI EaeI EagI
EciI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI