Restriction map for
pF1KB7953
(Linear) (Six-base) MAPSORT of: KIBB7953.seq Check: 5379 from: 1 to: 768
KIBB7953.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AvaI C'yCGr_G
Cuts at: 0 55 768
Size: 55 713
BamHI G'GATC_C
Cuts at: 0 149 768
Size: 149 619
BanII G_rGCy'C
Cuts at: 0 178 742 768
Size: 178 564 26
Fragments arranged by size:
564 178 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 338 768
Size: 338 430
BglI GCCn_nnn'nGGC
Cuts at: 0 231 768
Size: 231 537
BlpI GC'TnA_GC
Cuts at: 0 109 768
Size: 109 659
Bme1580I G_kGCm'C
Cuts at: 0 62 484 768
Size: 62 422 284
Fragments arranged by size:
422 284 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 727 768
Size: 220 507 41
Fragments arranged by size:
507 220 41
Bpu10I CC'TnA_GC
Cuts at: 0 11 768
Size: 11 757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 768
Size: 484 284
BsaAI yAC'GTr
Cuts at: 0 642 768
Size: 642 126
BsaHI Gr'CG_yC
Cuts at: 0 28 768
Size: 28 740
BsaWI w'CCGG_w
Cuts at: 0 668 768
Size: 668 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 768
Size: 265 30 473
Fragments arranged by size:
473 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 568 768
Size: 568 200
BsiHKAI G_wGCw'C
Cuts at: 0 641 742 768
Size: 641 101 26
Fragments arranged by size:
641 101 26
Bsp1286I G_dGCh'C
Cuts at: 0 62 178 484 641 742 768
Size: 62 116 306 157 101 26
Fragments arranged by size:
306 157 116 101 62 26
BspHI T'CATG_A
Cuts at: 0 569 734 768
Size: 569 165 34
Fragments arranged by size:
569 165 34
BsrFI r'CCGG_y
Cuts at: 0 6 630 768
Size: 6 624 138
Fragments arranged by size:
624 138 6
BssSI C'ACGA_G
Cuts at: 0 490 768
Size: 490 278
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 768
Size: 321 447
BstYI r'GATC_y
Cuts at: 0 149 768
Size: 149 619
Bsu36I CC'TnA_GG
Cuts at: 0 368 768
Size: 368 400
BtgI C'CryG_G
Cuts at: 0 209 314 768
Size: 209 105 454
Fragments arranged by size:
454 209 105
DrdI GACnn_nn'nnGTC
Cuts at: 0 27 768
Size: 27 741
EaeI y'GGCC_r
Cuts at: 0 563 768
Size: 563 205
EarI CTCTTCn'nnn_
Cuts at: 0 406 692 768
Size: 406 286 76
Fragments arranged by size:
406 286 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 649 768
Size: 649 119
EcoICRI GAG'CTC
Cuts at: 0 740 768
Size: 740 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 727 768
Size: 220 507 41
Fragments arranged by size:
507 220 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 619 768
Size: 619 149
EcoO109I rG'GnC_Cy
Cuts at: 0 51 768
Size: 51 717
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 525 768
Size: 525 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 768
Size: 265 32 471
Fragments arranged by size:
471 265 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 768
Size: 11 757
MscI TGG'CCA
Cuts at: 0 565 768
Size: 565 203
MspA1I CmG'CkG
Cuts at: 0 243 750 768
Size: 243 507 18
Fragments arranged by size:
507 243 18
NaeI GCC'GGC
Cuts at: 0 8 632 768
Size: 8 624 136
Fragments arranged by size:
624 136 8
NgoMIV G'CCGG_C
Cuts at: 0 6 630 768
Size: 6 624 138
Fragments arranged by size:
624 138 6
PfoI T'CCnGG_A
Cuts at: 0 704 768
Size: 704 64
PmlI CAC'GTG
Cuts at: 0 642 768
Size: 642 126
PpuMI rG'GwC_Cy
Cuts at: 0 51 768
Size: 51 717
PstI C_TGCA'G
Cuts at: 0 248 281 768
Size: 248 33 487
Fragments arranged by size:
487 248 33
PvuII CAG'CTG
Cuts at: 0 243 750 768
Size: 243 507 18
Fragments arranged by size:
507 243 18
SacI G_AGCT'C
Cuts at: 0 742 768
Size: 742 26
SfcI C'TryA_G
Cuts at: 0 244 277 288 768
Size: 244 33 11 480
Fragments arranged by size:
480 244 33 11
SgrAI Cr'CCGG_yG
Cuts at: 0 630 768
Size: 630 138
SmlI C'TyrA_G
Cuts at: 0 499 768
Size: 499 269
StuI AGG'CCT
Cuts at: 0 712 768
Size: 712 56
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 768
Size: 321 447
Enzymes that do cut:
AvaI BamHI BanII BbsI BglI BlpI Bme1580I BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsiHKAI
Bsp1286I BspHI BsrFI BssSI BstXI BstYI Bsu36I BtgI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI Hin4I MmeI MscI MspA1I NaeI NgoMIV PfoI
PmlI PpuMI PstI PvuII SacI SfcI SgrAI SmlI
StuI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BplI BsaI BsaBI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MslI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SmaI SnaBI SpeI SphI SrfI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI