Restriction map for
pF1KB7956
(Linear) (Six-base) MAPSORT of: KIBB7956.seq Check: 5246 from: 1 to: 1185
KIBB7956.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 629 1185
Size: 629 556
AccI GT'mk_AC
Cuts at: 0 954 1185
Size: 954 231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 921 1185
Size: 215 706 264
Fragments arranged by size:
706 264 215
AflIII A'CryG_T
Cuts at: 0 29 1185
Size: 29 1156
AlwNI CAG_nnn'CTG
Cuts at: 0 138 795 964 1185
Size: 138 657 169 221
Fragments arranged by size:
657 221 169 138
ApaI G_GGCC'C
Cuts at: 0 363 1185
Size: 363 822
BamHI G'GATC_C
Cuts at: 0 67 1185
Size: 67 1118
BanI G'GyrC_C
Cuts at: 0 444 511 1185
Size: 444 67 674
Fragments arranged by size:
674 444 67
BanII G_rGCy'C
Cuts at: 0 44 363 433 1185
Size: 44 319 70 752
Fragments arranged by size:
752 319 70 44
BglI GCCn_nnn'nGGC
Cuts at: 0 313 1185
Size: 313 872
BlpI GC'TnA_GC
Cuts at: 0 824 1185
Size: 824 361
Bme1580I G_kGCm'C
Cuts at: 0 363 530 1185
Size: 363 167 655
Fragments arranged by size:
655 363 167
BmrI ACTGGGnnnn_n'
Cuts at: 0 395 517 1139 1185
Size: 395 122 622 46
Fragments arranged by size:
622 395 122 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 86 97 385 754 817 1060 1100
Size: 58 28 11 288 369 63 243 40
Cuts at: 1100 1185
Size: 85
Fragments arranged by size:
369 288 243 85 63 58 40 28
11
Bpu10I CC'TnA_GC
Cuts at: 0 692 1185
Size: 692 493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1138 1185
Size: 637 501 47
Fragments arranged by size:
637 501 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 1036 1185
Size: 1036 149
BsaWI w'CCGG_w
Cuts at: 0 111 154 1157 1185
Size: 111 43 1003 28
Fragments arranged by size:
1003 111 43 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 309 339 796 826 1185
Size: 309 30 457 30 359
Fragments arranged by size:
457 359 309 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 589 655 1185
Size: 589 66 530
Fragments arranged by size:
589 530 66
BseYI C'CCAG_C
Cuts at: 0 81 432 588 931 964 1185
Size: 81 351 156 343 33 221
Fragments arranged by size:
351 343 221 156 81 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1185
Size: 743 442
Bsp1286I G_dGCh'C
Cuts at: 0 44 363 433 530 1185
Size: 44 319 70 97 655
Fragments arranged by size:
655 319 97 70 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 29 629 1185
Size: 29 600 556
Fragments arranged by size:
600 556 29
BsrGI T'GTAC_A
Cuts at: 0 532 1185
Size: 532 653
BstYI r'GATC_y
Cuts at: 0 3 67 1185
Size: 3 64 1118
Fragments arranged by size:
1118 64 3
BtgI C'CryG_G
Cuts at: 0 165 1185
Size: 165 1020
BtsI GCAGTG_nn'
Cuts at: 0 90 264 1111 1185
Size: 90 174 847 74
Fragments arranged by size:
847 174 90 74
DraIII CAC_nnn'GTG
Cuts at: 0 993 1185
Size: 993 192
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 723 820 1185
Size: 723 97 365
Fragments arranged by size:
723 365 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 86 97 215 385 754 817 921
Size: 58 28 11 118 170 369 63 104
Cuts at: 921 1060 1100 1185
Size: 139 40 85
Fragments arranged by size:
369 170 139 118 104 85 63 58
40 28 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 631 655 1185
Size: 631 24 530
Fragments arranged by size:
631 530 24
EcoO109I rG'GnC_Cy
Cuts at: 0 359 360 648 1185
Size: 359 1 288 537
Fragments arranged by size:
537 359 288 1
EcoRV GAT'ATC
Cuts at: 0 54 1185
Size: 54 1131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 1185
Size: 43 32 1110
Fragments arranged by size:
1110 43 32
HincII GTy'rAC
Cuts at: 0 955 1185
Size: 955 230
HindIII A'AGCT_T
Cuts at: 0 208 1185
Size: 208 977
MluI A'CGCG_T
Cuts at: 0 29 1185
Size: 29 1156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1185
Size: 887 298
MslI CAynn'nnrTG
Cuts at: 0 1175 1185
Size: 1175 10
MspA1I CmG'CkG
Cuts at: 0 837 1185
Size: 837 348
NspI r_CATG'y
Cuts at: 0 1174 1185
Size: 1174 11
PasI CC'CwG_GG
Cuts at: 0 311 1185
Size: 311 874
PflMI CCAn_nnn'nTGG
Cuts at: 0 795 964 1185
Size: 795 169 221
Fragments arranged by size:
795 221 169
PfoI T'CCnGG_A
Cuts at: 0 74 788 1185
Size: 74 714 397
Fragments arranged by size:
714 397 74
PpuMI rG'GwC_Cy
Cuts at: 0 648 1185
Size: 648 537
PspOMI G'GGCC_C
Cuts at: 0 359 1185
Size: 359 826
PstI C_TGCA'G
Cuts at: 0 842 1185
Size: 842 343
PvuII CAG'CTG
Cuts at: 0 837 1185
Size: 837 348
RsrII CG'GwC_CG
Cuts at: 0 1160 1185
Size: 1160 25
SalI G'TCGA_C
Cuts at: 0 953 1185
Size: 953 232
SfcI C'TryA_G
Cuts at: 0 124 838 1185
Size: 124 714 347
Fragments arranged by size:
714 347 124
SmlI C'TyrA_G
Cuts at: 0 652 1117 1185
Size: 652 465 68
Fragments arranged by size:
652 465 68
SphI G_CATG'C
Cuts at: 0 1174 1185
Size: 1174 11
StuI AGG'CCT
Cuts at: 0 401 1185
Size: 401 784
StyI C'CwwG_G
Cuts at: 0 632 815 1085 1185
Size: 632 183 270 100
Fragments arranged by size:
632 270 183 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 583 1185
Size: 583 602
TatI w'GTAC_w
Cuts at: 0 233 532 719 1185
Size: 233 299 187 466
Fragments arranged by size:
466 299 233 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 141 173 796 828 1185
Size: 141 32 623 32 357
Fragments arranged by size:
623 357 141 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 211 1185
Size: 211 974
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaI BamHI BanI
BanII BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspMI
BsrGI BstYI BtgI BtsI DraIII EciI Eco57MI EcoNI
EcoO109I EcoRV Hin4I HincII HindIII MluI MmeI MslI
MspA1I NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII RsrII SalI SfcI SmlI SphI StuI StyI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmtI BplI BsaAI BsaBI BsaHI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI
EagI EarI EcoICRI EcoRI FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI SacI SacII SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI