Restriction map for
pF1KB7957
(Linear) (Six-base) MAPSORT of: KIBB7957.seq Check: 6953 from: 1 to: 783
KIBB7957.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 124 783
Size: 124 659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 701 783
Size: 239 462 82
Fragments arranged by size:
462 239 82
AleI CACnn'nnGTG
Cuts at: 0 109 783
Size: 109 674
AlwNI CAG_nnn'CTG
Cuts at: 0 513 783
Size: 513 270
AvaI C'yCGr_G
Cuts at: 0 163 783
Size: 163 620
BamHI G'GATC_C
Cuts at: 0 446 783
Size: 446 337
BanII G_rGCy'C
Cuts at: 0 569 783
Size: 569 214
BbvCI CC'TCA_GC
Cuts at: 0 406 733 783
Size: 406 327 50
Fragments arranged by size:
406 327 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 783
Size: 783
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 490 783
Size: 362 128 293
Fragments arranged by size:
362 293 128
Bpu10I CC'TnA_GC
Cuts at: 0 295 406 733 783
Size: 295 111 327 50
Fragments arranged by size:
327 295 111 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 544 783
Size: 66 478 239
Fragments arranged by size:
478 239 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 724 783
Size: 724 59
BsaAI yAC'GTr
Cuts at: 0 258 783
Size: 258 525
BsaWI w'CCGG_w
Cuts at: 0 428 783
Size: 428 355
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 712 783
Size: 712 71
BseYI C'CCAG_C
Cuts at: 0 13 247 783
Size: 13 234 536
Fragments arranged by size:
536 234 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 783
Size: 649 134
BsiEI CG_ry'CG
Cuts at: 0 146 783
Size: 146 637
BsmBI CGTCTCn'nnnn_
Cuts at: 0 189 783
Size: 189 594
Bsp1286I G_dGCh'C
Cuts at: 0 569 783
Size: 569 214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 124 783
Size: 124 659
BsrBI CCG'CTC
Cuts at: 0 594 783
Size: 594 189
BsrGI T'GTAC_A
Cuts at: 0 473 783
Size: 473 310
BstXI CCAn_nnnn'nTGG
Cuts at: 0 111 647 783
Size: 111 536 136
Fragments arranged by size:
536 136 111
BstYI r'GATC_y
Cuts at: 0 446 783
Size: 446 337
BtgI C'CryG_G
Cuts at: 0 80 110 273 783
Size: 80 30 163 510
Fragments arranged by size:
510 163 80 30
BtsI GCAGTG_nn'
Cuts at: 0 450 783
Size: 450 333
DrdI GACnn_nn'nnGTC
Cuts at: 0 213 763 783
Size: 213 550 20
Fragments arranged by size:
550 213 20
EaeI y'GGCC_r
Cuts at: 0 143 260 783
Size: 143 117 523
Fragments arranged by size:
523 143 117
EagI C'GGCC_G
Cuts at: 0 143 783
Size: 143 640
EarI CTCTTCn'nnn_
Cuts at: 0 710 783
Size: 710 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 391 553 783
Size: 391 162 230
Fragments arranged by size:
391 230 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 362 490 701 783
Size: 239 123 128 211 82
Fragments arranged by size:
239 211 128 123 82
EcoNI CCTnn'n_nnAGG
Cuts at: 0 559 783
Size: 559 224
EcoO109I rG'GnC_Cy
Cuts at: 0 70 291 347 510 783
Size: 70 221 56 163 273
Fragments arranged by size:
273 221 163 70 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 783
Size: 511 32 240
Fragments arranged by size:
511 240 32
HindIII A'AGCT_T
Cuts at: 0 42 783
Size: 42 741
MscI TGG'CCA
Cuts at: 0 262 783
Size: 262 521
MslI CAynn'nnrTG
Cuts at: 0 109 783
Size: 109 674
NcoI C'CATG_G
Cuts at: 0 80 273 783
Size: 80 193 510
Fragments arranged by size:
510 193 80
PasI CC'CwG_GG
Cuts at: 0 117 783
Size: 117 666
PflMI CCAn_nnn'nTGG
Cuts at: 0 513 783
Size: 513 270
PfoI T'CCnGG_A
Cuts at: 0 359 722 783
Size: 359 363 61
Fragments arranged by size:
363 359 61
PmlI CAC'GTG
Cuts at: 0 258 783
Size: 258 525
PpuMI rG'GwC_Cy
Cuts at: 0 70 783
Size: 70 713
PshAI GACnn'nnGTC
Cuts at: 0 181 783
Size: 181 602
PstI C_TGCA'G
Cuts at: 0 581 783
Size: 581 202
SfcI C'TryA_G
Cuts at: 0 441 577 783
Size: 441 136 206
Fragments arranged by size:
441 206 136
SmlI C'TyrA_G
Cuts at: 0 45 559 783
Size: 45 514 224
Fragments arranged by size:
514 224 45
StyI C'CwwG_G
Cuts at: 0 60 80 273 783
Size: 60 20 193 510
Fragments arranged by size:
510 193 60 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 266 783
Size: 266 517
TatI w'GTAC_w
Cuts at: 0 473 783
Size: 473 310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 783
Size: 255 528
Enzymes that do cut:
AarI AcuI AleI AlwNI AvaI BamHI BanII BbvCI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BseRI
BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI BsrGI
BstXI BstYI BtgI BtsI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I Hin4I HindIII MscI MslI
NcoI PasI PflMI PfoI PmlI PpuMI PshAI PstI
SfcI SmlI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BsaBI BsaHI BsaXI BsiHKAI BsiWI BsmI BspEI
BspHI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI