Restriction map for
pF1KB7958
(Linear) (Six-base) MAPSORT of: KIBB7958.seq Check: 8344 from: 1 to: 1188
KIBB7958.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AflII C'TTAA_G
Cuts at: 0 988 1188
Size: 988 200
AflIII A'CryG_T
Cuts at: 0 173 247 1188
Size: 173 74 941
Fragments arranged by size:
941 173 74
AhdI GACnn_n'nnGTC
Cuts at: 0 519 1188
Size: 519 669
AleI CACnn'nnGTG
Cuts at: 0 128 841 1188
Size: 128 713 347
Fragments arranged by size:
713 347 128
AlwNI CAG_nnn'CTG
Cuts at: 0 1064 1108 1188
Size: 1064 44 80
Fragments arranged by size:
1064 80 44
ApoI r'AATT_y
Cuts at: 0 886 916 1032 1188
Size: 886 30 116 156
Fragments arranged by size:
886 156 116 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 304 337 1188
Size: 304 33 851
Fragments arranged by size:
851 304 33
BamHI G'GATC_C
Cuts at: 0 526 1188
Size: 526 662
BciVI GTATCCnnnnn_n'
Cuts at: 0 332 375 1188
Size: 332 43 813
Fragments arranged by size:
813 332 43
BclI T'GATC_A
Cuts at: 0 678 1188
Size: 678 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 434 1185 1188
Size: 210 224 751 3
Fragments arranged by size:
751 224 210 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1188
Size: 238 950
BsaI GGTCTCn'nnnn_
Cuts at: 0 587 1188
Size: 587 601
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 761 791 1158 1188
Size: 761 30 367 30
Fragments arranged by size:
761 367 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 1188
Size: 769 419
BseYI C'CCAG_C
Cuts at: 0 3 1188
Size: 3 1185
BspHI T'CATG_A
Cuts at: 0 327 1188
Size: 327 861
BsrDI GCAATG_nn'
Cuts at: 0 944 1188
Size: 944 244
BsrGI T'GTAC_A
Cuts at: 0 312 1188
Size: 312 876
BstAPI GCAn_nnn'nTGC
Cuts at: 0 724 1064 1188
Size: 724 340 124
Fragments arranged by size:
724 340 124
BstXI CCAn_nnnn'nTGG
Cuts at: 0 130 1188
Size: 130 1058
BstYI r'GATC_y
Cuts at: 0 526 1188
Size: 526 662
BtgI C'CryG_G
Cuts at: 0 123 1188
Size: 123 1065
BtsI GCAGTG_nn'
Cuts at: 0 263 722 1179 1188
Size: 263 459 457 9
Fragments arranged by size:
459 457 263 9
EarI CTCTTCn'nnn_
Cuts at: 0 50 645 1188
Size: 50 595 543
Fragments arranged by size:
595 543 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 434 1185 1188
Size: 210 224 751 3
Fragments arranged by size:
751 224 210 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 161 1188
Size: 161 1027
EcoO109I rG'GnC_Cy
Cuts at: 0 39 493 1188
Size: 39 454 695
Fragments arranged by size:
695 454 39
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 523 1188
Size: 523 665
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 761 793 1188
Size: 505 32 224 32 395
Fragments arranged by size:
505 395 224 32 32
HincII GTy'rAC
Cuts at: 0 68 1011 1188
Size: 68 943 177
Fragments arranged by size:
943 177 68
MslI CAynn'nnrTG
Cuts at: 0 128 284 337 660 841 1188
Size: 128 156 53 323 181 347
Fragments arranged by size:
347 323 181 156 128 53
NdeI CA'TA_TG
Cuts at: 0 443 933 1188
Size: 443 490 255
Fragments arranged by size:
490 443 255
NspI r_CATG'y
Cuts at: 0 177 251 342 1188
Size: 177 74 91 846
Fragments arranged by size:
846 177 91 74
PciI A'CATG_T
Cuts at: 0 173 247 1188
Size: 173 74 941
Fragments arranged by size:
941 173 74
PfoI T'CCnGG_A
Cuts at: 0 1037 1188
Size: 1037 151
PpuMI rG'GwC_Cy
Cuts at: 0 39 493 1188
Size: 39 454 695
Fragments arranged by size:
695 454 39
PsiI TTA'TAA
Cuts at: 0 1001 1188
Size: 1001 187
PstI C_TGCA'G
Cuts at: 0 405 910 1188
Size: 405 505 278
Fragments arranged by size:
505 405 278
SapI GCTCTTCn'nnn_
Cuts at: 0 645 1188
Size: 645 543
SfcI C'TryA_G
Cuts at: 0 401 544 906 1188
Size: 401 143 362 282
Fragments arranged by size:
401 362 282 143
SmlI C'TyrA_G
Cuts at: 0 217 988 1188
Size: 217 771 200
Fragments arranged by size:
771 217 200
SspI AAT'ATT
Cuts at: 0 566 1188
Size: 566 622
TatI w'GTAC_w
Cuts at: 0 204 312 501 1188
Size: 204 108 189 687
Fragments arranged by size:
687 204 189 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1158 1188
Size: 1158 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 468 1188
Size: 468 720
XmnI GAAnn'nnTTC
Cuts at: 0 1103 1188
Size: 1103 85
Enzymes that do cut:
AflII AflIII AhdI AleI AlwNI ApoI BaeI BamHI
BciVI BclI BpmI BpuEI BsaI BsaXI BseRI BseYI
BspHI BsrDI BsrGI BstAPI BstXI BstYI BtgI BtsI
EarI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MslI
NdeI NspI PciI PfoI PpuMI PsiI PstI SapI
SfcI SmlI SspI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BanI BanII BbeI BbsI BbvCI BcgI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII
Tth111I XbaI XhoI XmaI ZraI