Restriction map for
pF1KB7965
(Linear) (Six-base) MAPSORT of: KIBB7965.seq Check: 9964 from: 1 to: 840
KIBB7965.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 740 840
Size: 740 100
Acc65I G'GTAC_C
Cuts at: 0 663 840
Size: 663 177
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 324 840
Size: 214 110 516
Fragments arranged by size:
516 214 110
AleI CACnn'nnGTG
Cuts at: 0 763 840
Size: 763 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 430 840
Size: 430 410
AlwNI CAG_nnn'CTG
Cuts at: 0 94 840
Size: 94 746
ApaI G_GGCC'C
Cuts at: 0 772 840
Size: 772 68
ApoI r'AATT_y
Cuts at: 0 24 840
Size: 24 816
AvaI C'yCGr_G
Cuts at: 0 466 571 840
Size: 466 105 269
Fragments arranged by size:
466 269 105
BanI G'GyrC_C
Cuts at: 0 492 582 598 663 757 840
Size: 492 90 16 65 94 83
Fragments arranged by size:
492 94 90 83 65 16
BanII G_rGCy'C
Cuts at: 0 772 840
Size: 772 68
BbeI G_GCGC'C
Cuts at: 0 496 586 602 840
Size: 496 90 16 238
Fragments arranged by size:
496 238 90 16
BglI GCCn_nnn'nGGC
Cuts at: 0 490 840
Size: 490 350
BlpI GC'TnA_GC
Cuts at: 0 298 840
Size: 298 542
Bme1580I G_kGCm'C
Cuts at: 0 566 772 840
Size: 566 206 68
Fragments arranged by size:
566 206 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 647 840
Size: 244 403 193
Fragments arranged by size:
403 244 193
Bpu10I CC'TnA_GC
Cuts at: 0 638 840
Size: 638 202
BsaAI yAC'GTr
Cuts at: 0 380 840
Size: 380 460
BsaHI Gr'CG_yC
Cuts at: 0 493 583 599 840
Size: 493 90 16 241
Fragments arranged by size:
493 241 90 16
BsaWI w'CCGG_w
Cuts at: 0 72 263 840
Size: 72 191 577
Fragments arranged by size:
577 191 72
BseYI C'CCAG_C
Cuts at: 0 51 318 527 612 840
Size: 51 267 209 85 228
Fragments arranged by size:
267 228 209 85 51
BsiHKAI G_wGCw'C
Cuts at: 0 101 304 840
Size: 101 203 536
Fragments arranged by size:
536 203 101
BsmI GAATG_Cn'
Cuts at: 0 213 840
Size: 213 627
Bsp1286I G_dGCh'C
Cuts at: 0 101 304 566 772 840
Size: 101 203 262 206 68
Fragments arranged by size:
262 206 203 101 68
BspEI T'CCGG_A
Cuts at: 0 263 840
Size: 263 577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 278 840
Size: 278 562
BsrFI r'CCGG_y
Cuts at: 0 334 666 840
Size: 334 332 174
Fragments arranged by size:
334 332 174
BssSI C'ACGA_G
Cuts at: 0 559 840
Size: 559 281
BstEII G'GTnAC_C
Cuts at: 0 360 840
Size: 360 480
BtgI C'CryG_G
Cuts at: 0 764 829 840
Size: 764 65 11
Fragments arranged by size:
764 65 11
BtsI GCAGTG_nn'
Cuts at: 0 785 840
Size: 785 55
EaeI y'GGCC_r
Cuts at: 0 420 428 678 840
Size: 420 8 250 162
Fragments arranged by size:
420 250 162 8
EarI CTCTTCn'nnn_
Cuts at: 0 132 840
Size: 132 708
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 247 826 840
Size: 247 579 14
Fragments arranged by size:
579 247 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 244 324 647 840
Size: 214 30 80 323 193
Fragments arranged by size:
323 214 193 80 30
EcoO109I rG'GnC_Cy
Cuts at: 0 514 707 769 840
Size: 514 193 62 71
Fragments arranged by size:
514 193 71 62
FspI TGC'GCA
Cuts at: 0 406 840
Size: 406 434
FspAI rTGC'GCAy
Cuts at: 0 406 840
Size: 406 434
HaeII r_GCGC'y
Cuts at: 0 496 586 602 840
Size: 496 90 16 238
Fragments arranged by size:
496 238 90 16
HincII GTy'rAC
Cuts at: 0 63 840
Size: 63 777
KasI G'GCGC_C
Cuts at: 0 492 582 598 840
Size: 492 90 16 242
Fragments arranged by size:
492 242 90 16
KpnI G_GTAC'C
Cuts at: 0 667 840
Size: 667 173
MscI TGG'CCA
Cuts at: 0 430 680 840
Size: 430 250 160
Fragments arranged by size:
430 250 160
MslI CAynn'nnrTG
Cuts at: 0 763 840
Size: 763 77
MspA1I CmG'CkG
Cuts at: 0 7 168 276 722 755 794 840
Size: 7 161 108 446 33 39 46
Fragments arranged by size:
446 161 108 46 39 33 7
NaeI GCC'GGC
Cuts at: 0 336 840
Size: 336 504
NarI GG'CG_CC
Cuts at: 0 493 583 599 840
Size: 493 90 16 241
Fragments arranged by size:
493 241 90 16
NgoMIV G'CCGG_C
Cuts at: 0 334 840
Size: 334 506
PmlI CAC'GTG
Cuts at: 0 380 840
Size: 380 460
PpuMI rG'GwC_Cy
Cuts at: 0 514 840
Size: 514 326
PspOMI G'GGCC_C
Cuts at: 0 768 840
Size: 768 72
PstI C_TGCA'G
Cuts at: 0 275 840
Size: 275 565
PvuII CAG'CTG
Cuts at: 0 7 168 840
Size: 7 161 672
Fragments arranged by size:
672 161 7
SanDI GG'GwC_CC
Cuts at: 0 514 840
Size: 514 326
SapI GCTCTTCn'nnn_
Cuts at: 0 132 840
Size: 132 708
SfcI C'TryA_G
Cuts at: 0 271 840
Size: 271 569
SfoI GGC'GCC
Cuts at: 0 494 584 600 840
Size: 494 90 16 240
Fragments arranged by size:
494 240 90 16
SmaI CCC'GGG
Cuts at: 0 468 573 840
Size: 468 105 267
Fragments arranged by size:
468 267 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 805 837 840
Size: 805 32 3
Fragments arranged by size:
805 32 3
XmaI C'CCGG_G
Cuts at: 0 466 571 840
Size: 466 105 269
Fragments arranged by size:
466 269 105
Enzymes that do cut:
AccI Acc65I AcuI AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BglI BlpI Bme1580I BpmI
Bpu10I BsaAI BsaHI BsaWI BseYI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrFI BssSI BstEII BtgI BtsI EaeI
EarI EciI Eco57MI EcoO109I FspI FspAI HaeII HincII
KasI KpnI MscI MslI MspA1I NaeI NarI NgoMIV
PmlI PpuMI PspOMI PstI PvuII SanDI SapI SfcI
SfoI SmaI TstI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BplI BpuEI BsaI BsaBI BsaXI BseRI
BsgI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI Hin4I HindIII HpaI MfeI
MluI MmeI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmnI ZraI