Restriction map for
pF1KB7966
(Linear) (Six-base) MAPSORT of: KIBB7966.seq Check: 4212 from: 1 to: 1203
KIBB7966.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 619 1203
Size: 619 584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1110 1168 1203
Size: 560 550 58 35
Fragments arranged by size:
560 550 58 35
AlwNI CAG_nnn'CTG
Cuts at: 0 418 1203
Size: 418 785
ApaI G_GGCC'C
Cuts at: 0 311 1203
Size: 311 892
AvaI C'yCGr_G
Cuts at: 0 103 873 1203
Size: 103 770 330
Fragments arranged by size:
770 330 103
AvrII C'CTAG_G
Cuts at: 0 840 1203
Size: 840 363
BanI G'GyrC_C
Cuts at: 0 377 1203
Size: 377 826
BanII G_rGCy'C
Cuts at: 0 40 58 311 751 1203
Size: 40 18 253 440 452
Fragments arranged by size:
452 440 253 40 18
BbeI G_GCGC'C
Cuts at: 0 381 1203
Size: 381 822
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 551 1203
Size: 517 34 652
Fragments arranged by size:
652 517 34
BglI GCCn_nnn'nGGC
Cuts at: 0 315 1203
Size: 315 888
Bme1580I G_kGCm'C
Cuts at: 0 279 311 1203
Size: 279 32 892
Fragments arranged by size:
892 279 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 108 1203
Size: 108 1095
Bpu10I CC'TnA_GC
Cuts at: 0 483 860 1203
Size: 483 377 343
Fragments arranged by size:
483 377 343
BsaI GGTCTCn'nnnn_
Cuts at: 0 793 1203
Size: 793 410
BsaBI GATnn'nnATC
Cuts at: 0 701 769 1203
Size: 701 68 434
Fragments arranged by size:
701 434 68
BsaHI Gr'CG_yC
Cuts at: 0 142 378 1203
Size: 142 236 825
Fragments arranged by size:
825 236 142
BsaWI w'CCGG_w
Cuts at: 0 1107 1203
Size: 1107 96
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 236 362 655 811 1082 1203
Size: 236 126 293 156 271 121
Fragments arranged by size:
293 271 236 156 126 121
BseYI C'CCAG_C
Cuts at: 0 183 291 304 602 823 1203
Size: 183 108 13 298 221 380
Fragments arranged by size:
380 298 221 183 108 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1203
Size: 547 656
BsiEI CG_ry'CG
Cuts at: 0 319 962 1203
Size: 319 643 241
Fragments arranged by size:
643 319 241
BsiHKAI G_wGCw'C
Cuts at: 0 121 557 751 1203
Size: 121 436 194 452
Fragments arranged by size:
452 436 194 121
Bsp1286I G_dGCh'C
Cuts at: 0 40 58 121 279 311 557 751 1203
Size: 40 18 63 158 32 246 194 452
Fragments arranged by size:
452 246 194 158 63 40 32 18
BsrFI r'CCGG_y
Cuts at: 0 252 314 1203
Size: 252 62 889
Fragments arranged by size:
889 252 62
BsrGI T'GTAC_A
Cuts at: 0 817 1203
Size: 817 386
BssHII G'CGCG_C
Cuts at: 0 256 1203
Size: 256 947
BstXI CCAn_nnnn'nTGG
Cuts at: 0 191 1203
Size: 191 1012
BstYI r'GATC_y
Cuts at: 0 696 1203
Size: 696 507
BtgI C'CryG_G
Cuts at: 0 85 128 159 190 337 723 1203
Size: 85 43 31 31 147 386 480
Fragments arranged by size:
480 386 147 85 43 31 31
BtsI GCAGTG_nn'
Cuts at: 0 429 1203
Size: 429 774
DraIII CAC_nnn'GTG
Cuts at: 0 1075 1203
Size: 1075 128
EaeI y'GGCC_r
Cuts at: 0 316 998 1203
Size: 316 682 205
Fragments arranged by size:
682 316 205
EagI C'GGCC_G
Cuts at: 0 316 1203
Size: 316 887
EcoICRI GAG'CTC
Cuts at: 0 749 1203
Size: 749 454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1110 1168 1203
Size: 560 550 58 35
Fragments arranged by size:
560 550 58 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 514 1117 1203
Size: 514 603 86
Fragments arranged by size:
603 514 86
EcoO109I rG'GnC_Cy
Cuts at: 0 227 308 1203
Size: 227 81 895
Fragments arranged by size:
895 227 81
HaeII r_GCGC'y
Cuts at: 0 190 381 1203
Size: 190 191 822
Fragments arranged by size:
822 191 190
KasI G'GCGC_C
Cuts at: 0 377 1203
Size: 377 826
MspA1I CmG'CkG
Cuts at: 0 161 339 478 823 1203
Size: 161 178 139 345 380
Fragments arranged by size:
380 345 178 161 139
NaeI GCC'GGC
Cuts at: 0 316 1203
Size: 316 887
NarI GG'CG_CC
Cuts at: 0 378 1203
Size: 378 825
NcoI C'CATG_G
Cuts at: 0 85 1203
Size: 85 1118
NgoMIV G'CCGG_C
Cuts at: 0 314 1203
Size: 314 889
NspI r_CATG'y
Cuts at: 0 678 1203
Size: 678 525
PasI CC'CwG_GG
Cuts at: 0 351 1203
Size: 351 852
PfoI T'CCnGG_A
Cuts at: 0 386 1203
Size: 386 817
PpuMI rG'GwC_Cy
Cuts at: 0 227 1203
Size: 227 976
PspOMI G'GGCC_C
Cuts at: 0 307 1203
Size: 307 896
PstI C_TGCA'G
Cuts at: 0 1173 1203
Size: 1173 30
PvuII CAG'CTG
Cuts at: 0 823 1203
Size: 823 380
SacI G_AGCT'C
Cuts at: 0 751 1203
Size: 751 452
SacII CC_GC'GG
Cuts at: 0 162 340 1203
Size: 162 178 863
Fragments arranged by size:
863 178 162
SfcI C'TryA_G
Cuts at: 0 625 1169 1203
Size: 625 544 34
Fragments arranged by size:
625 544 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 315 1203
Size: 315 888
SfoI GGC'GCC
Cuts at: 0 379 1203
Size: 379 824
SgrAI Cr'CCGG_yG
Cuts at: 0 252 1203
Size: 252 951
SmaI CCC'GGG
Cuts at: 0 105 875 1203
Size: 105 770 328
Fragments arranged by size:
770 328 105
StyI C'CwwG_G
Cuts at: 0 85 299 458 515 840 1203
Size: 85 214 159 57 325 363
Fragments arranged by size:
363 325 214 159 85 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 948 1203
Size: 948 255
TatI w'GTAC_w
Cuts at: 0 817 1203
Size: 817 386
XbaI T'CTAG_A
Cuts at: 0 792 1203
Size: 792 411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 135 1126 1203
Size: 135 991 77
Fragments arranged by size:
991 135 77
XmaI C'CCGG_G
Cuts at: 0 103 873 1203
Size: 103 770 330
Fragments arranged by size:
770 330 103
Enzymes that do cut:
AccI AcuI AlwNI ApaI AvaI AvrII BanI BanII
BbeI BcgI BglI Bme1580I BmrI Bpu10I BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BsrFI BsrGI BssHII BstXI BstYI BtgI BtsI DraIII
EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SfcI SfiI
SfoI SgrAI SmaI StyI TaqII TatI XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaAI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EarI EciI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SgfI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XhoI XmnI ZraI