Restriction map for
pF1KB7967
(Linear) (Six-base) MAPSORT of: KIBB7967.seq Check: 4662 from: 1 to: 849
KIBB7967.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AclI AA'CG_TT
Cuts at: 0 120 849
Size: 120 729
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 849
Size: 256 593
AgeI A'CCGG_T
Cuts at: 0 599 849
Size: 599 250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 110 142 849
Size: 110 32 707
Fragments arranged by size:
707 110 32
ApaLI G'TGCA_C
Cuts at: 0 46 595 849
Size: 46 549 254
Fragments arranged by size:
549 254 46
ApoI r'AATT_y
Cuts at: 0 166 849
Size: 166 683
AvaI C'yCGr_G
Cuts at: 0 209 276 408 496 849
Size: 209 67 132 88 353
Fragments arranged by size:
353 209 132 88 67
BanI G'GyrC_C
Cuts at: 0 602 842 849
Size: 602 240 7
Fragments arranged by size:
602 240 7
BanII G_rGCy'C
Cuts at: 0 38 140 424 849
Size: 38 102 284 425
Fragments arranged by size:
425 284 102 38
BglII A'GATC_T
Cuts at: 0 183 849
Size: 183 666
Bme1580I G_kGCm'C
Cuts at: 0 50 497 599 849
Size: 50 447 102 250
Fragments arranged by size:
447 250 102 50
BmgBI CAC'GTC
Cuts at: 0 6 849
Size: 6 843
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 525 849
Size: 525 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 103 112 239 849
Size: 22 81 9 127 610
Fragments arranged by size:
610 127 81 22 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 292 849
Size: 46 246 557
Fragments arranged by size:
557 246 46
BsaWI w'CCGG_w
Cuts at: 0 453 599 849
Size: 453 146 250
Fragments arranged by size:
453 250 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 110 140 849
Size: 110 30 709
Fragments arranged by size:
709 110 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 414 603 849
Size: 100 314 189 246
Fragments arranged by size:
314 246 189 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 814 849
Size: 39 775 35
Fragments arranged by size:
775 39 35
BsiHKAI G_wGCw'C
Cuts at: 0 11 38 50 424 599 849
Size: 11 27 12 374 175 250
Fragments arranged by size:
374 250 175 27 12 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 83 849
Size: 83 766
Bsp1286I G_dGCh'C
Cuts at: 0 11 38 50 140 424 497 599 849
Size: 11 27 12 90 284 73 102 250
Fragments arranged by size:
284 250 102 90 73 27 12 11
BspEI T'CCGG_A
Cuts at: 0 453 849
Size: 453 396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 112 849
Size: 112 737
BsrFI r'CCGG_y
Cuts at: 0 55 599 849
Size: 55 544 250
Fragments arranged by size:
544 250 55
BssSI C'ACGA_G
Cuts at: 0 49 849
Size: 49 800
BstYI r'GATC_y
Cuts at: 0 183 849
Size: 183 666
BtgI C'CryG_G
Cuts at: 0 362 849
Size: 362 487
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 581 849
Size: 581 268
DraI TTT'AAA
Cuts at: 0 780 849
Size: 780 69
EaeI y'GGCC_r
Cuts at: 0 365 849
Size: 365 484
EarI CTCTTCn'nnn_
Cuts at: 0 832 849
Size: 832 17
EcoICRI GAG'CTC
Cuts at: 0 36 422 849
Size: 36 386 427
Fragments arranged by size:
427 386 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 103 112 239 256 849
Size: 22 81 9 127 17 593
Fragments arranged by size:
593 127 81 22 17 9
EcoRV GAT'ATC
Cuts at: 0 242 849
Size: 242 607
FspI TGC'GCA
Cuts at: 0 526 849
Size: 526 323
HaeII r_GCGC'y
Cuts at: 0 73 849
Size: 73 776
HincII GTy'rAC
Cuts at: 0 740 849
Size: 740 109
HpaI GTT'AAC
Cuts at: 0 740 849
Size: 740 109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 849
Size: 404 445
MspA1I CmG'CkG
Cuts at: 0 530 663 849
Size: 530 133 186
Fragments arranged by size:
530 186 133
PfoI T'CCnGG_A
Cuts at: 0 29 849
Size: 29 820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 849
Size: 110 32 707
Fragments arranged by size:
707 110 32
PstI C_TGCA'G
Cuts at: 0 622 849
Size: 622 227
SacI G_AGCT'C
Cuts at: 0 38 424 849
Size: 38 386 425
Fragments arranged by size:
425 386 38
ScaI AGT'ACT
Cuts at: 0 91 849
Size: 91 758
SfcI C'TryA_G
Cuts at: 0 618 849
Size: 618 231
SgrAI Cr'CCGG_yG
Cuts at: 0 599 849
Size: 599 250
SmaI CCC'GGG
Cuts at: 0 498 849
Size: 498 351
TatI w'GTAC_w
Cuts at: 0 89 849
Size: 89 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 172 204 534 566 849
Size: 172 32 330 32 283
Fragments arranged by size:
330 283 172 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 849
Size: 201 648
XmaI C'CCGG_G
Cuts at: 0 496 849
Size: 496 353
Enzymes that do cut:
AclI AcuI AgeI AloI ApaLI ApoI AvaI BanI
BanII BglII Bme1580I BmgBI BplI BpmI BsaI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrFI BssSI BstYI BtgI BtgZI DraI EaeI EarI
EcoICRI Eco57MI EcoRV FspI HaeII HincII HpaI MmeI
MspA1I PfoI PpiI PstI SacI ScaI SfcI SgrAI
SmaI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AhdI
AleI AlfI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmrI BmtI Bpu10I BpuEI BsaAI
BsaBI BsaHI BseYI BsiEI BsiWI BsmI BspHI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI FalI FseI FspAI Hin4I HindIII
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII Tth111I XbaI XhoI XmnI ZraI