Restriction map for
pF1KB7968
(Linear) (Six-base) MAPSORT of: KIBB7968.seq Check: 4199 from: 1 to: 1221
KIBB7968.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 129 1042 1126 1221
Size: 59 70 913 84 95
Fragments arranged by size:
913 95 84 70 59
AflIII A'CryG_T
Cuts at: 0 1061 1221
Size: 1061 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 946 1221
Size: 946 275
AlwNI CAG_nnn'CTG
Cuts at: 0 124 282 471 1221
Size: 124 158 189 750
Fragments arranged by size:
750 189 158 124
ApaI G_GGCC'C
Cuts at: 0 97 1221
Size: 97 1124
ApoI r'AATT_y
Cuts at: 0 1011 1221
Size: 1011 210
AvaI C'yCGr_G
Cuts at: 0 229 311 1221
Size: 229 82 910
Fragments arranged by size:
910 229 82
AvrII C'CTAG_G
Cuts at: 0 496 1221
Size: 496 725
BamHI G'GATC_C
Cuts at: 0 1121 1221
Size: 1121 100
BanII G_rGCy'C
Cuts at: 0 97 131 520 565 1183 1221
Size: 97 34 389 45 618 38
Fragments arranged by size:
618 389 97 45 38 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 191 225 1221
Size: 191 34 996
Fragments arranged by size:
996 191 34
BglII A'GATC_T
Cuts at: 0 504 1221
Size: 504 717
BlpI GC'TnA_GC
Cuts at: 0 559 960 1221
Size: 559 401 261
Fragments arranged by size:
559 401 261
Bme1580I G_kGCm'C
Cuts at: 0 97 1221
Size: 97 1124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 785 1221
Size: 207 578 436
Fragments arranged by size:
578 436 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1151 1221
Size: 506 645 70
Fragments arranged by size:
645 506 70
Bpu10I CC'TnA_GC
Cuts at: 0 125 1221
Size: 125 1096
BsaI GGTCTCn'nnnn_
Cuts at: 0 1047 1221
Size: 1047 174
BsaAI yAC'GTr
Cuts at: 0 1007 1064 1221
Size: 1007 57 157
Fragments arranged by size:
1007 157 57
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 89 580 1221
Size: 89 491 641
Fragments arranged by size:
641 491 89
BseYI C'CCAG_C
Cuts at: 0 194 616 1221
Size: 194 422 605
Fragments arranged by size:
605 422 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 716 1096 1221
Size: 220 496 380 125
Fragments arranged by size:
496 380 220 125
BsiHKAI G_wGCw'C
Cuts at: 0 131 434 520 1183 1221
Size: 131 303 86 663 38
Fragments arranged by size:
663 303 131 86 38
Bsp1286I G_dGCh'C
Cuts at: 0 97 131 434 520 565 1183 1221
Size: 97 34 303 86 45 618 38
Fragments arranged by size:
618 303 97 86 45 38 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 777 1221
Size: 777 444
BsrBI CCG'CTC
Cuts at: 0 959 1221
Size: 959 262
BsrDI GCAATG_nn'
Cuts at: 0 834 1221
Size: 834 387
BsrFI r'CCGG_y
Cuts at: 0 554 1221
Size: 554 667
BssSI C'ACGA_G
Cuts at: 0 358 1221
Size: 358 863
BstAPI GCAn_nnn'nTGC
Cuts at: 0 429 1221
Size: 429 792
BstEII G'GTnAC_C
Cuts at: 0 943 1221
Size: 943 278
BstXI CCAn_nnnn'nTGG
Cuts at: 0 398 1221
Size: 398 823
BstYI r'GATC_y
Cuts at: 0 120 302 448 504 712 1121 1221
Size: 120 182 146 56 208 409 100
Fragments arranged by size:
409 208 182 146 120 100 56
BtgI C'CryG_G
Cuts at: 0 1212 1221
Size: 1212 9
BtsI GCAGTG_nn'
Cuts at: 0 403 704 1221
Size: 403 301 517
Fragments arranged by size:
517 403 301
ClaI AT'CG_AT
Cuts at: 0 324 1221
Size: 324 897
EaeI y'GGCC_r
Cuts at: 0 404 1152 1221
Size: 404 748 69
Fragments arranged by size:
748 404 69
EarI CTCTTCn'nnn_
Cuts at: 0 77 1018 1221
Size: 77 941 203
Fragments arranged by size:
941 203 77
EcoICRI GAG'CTC
Cuts at: 0 129 518 1181 1221
Size: 129 389 663 40
Fragments arranged by size:
663 389 129 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 129 506 1042 1126 1151 1221
Size: 59 70 377 536 84 25 70
Fragments arranged by size:
536 377 84 70 70 59 25
EcoO109I rG'GnC_Cy
Cuts at: 0 93 94 627 1221
Size: 93 1 533 594
Fragments arranged by size:
594 533 93 1
EcoRI G'AATT_C
Cuts at: 0 1011 1221
Size: 1011 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 450 1221
Size: 450 771
HaeII r_GCGC'y
Cuts at: 0 190 1221
Size: 190 1031
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 294 326 552 584 1221
Size: 294 32 226 32 637
Fragments arranged by size:
637 294 226 32 32
HincII GTy'rAC
Cuts at: 0 1100 1221
Size: 1100 121
HindIII A'AGCT_T
Cuts at: 0 41 999 1167 1221
Size: 41 958 168 54
Fragments arranged by size:
958 168 54 41
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 225 1221
Size: 56 169 996
Fragments arranged by size:
996 169 56
MscI TGG'CCA
Cuts at: 0 1154 1221
Size: 1154 67
MslI CAynn'nnrTG
Cuts at: 0 1146 1221
Size: 1146 75
MspA1I CmG'CkG
Cuts at: 0 706 1221
Size: 706 515
NcoI C'CATG_G
Cuts at: 0 1212 1221
Size: 1212 9
PmlI CAC'GTG
Cuts at: 0 1007 1064 1221
Size: 1007 57 157
Fragments arranged by size:
1007 157 57
PspOMI G'GGCC_C
Cuts at: 0 93 1221
Size: 93 1128
PstI C_TGCA'G
Cuts at: 0 734 1221
Size: 734 487
PvuII CAG'CTG
Cuts at: 0 706 1221
Size: 706 515
SacI G_AGCT'C
Cuts at: 0 131 520 1183 1221
Size: 131 389 663 38
Fragments arranged by size:
663 389 131 38
SfcI C'TryA_G
Cuts at: 0 730 1221
Size: 730 491
StyI C'CwwG_G
Cuts at: 0 242 496 1212 1221
Size: 242 254 716 9
Fragments arranged by size:
716 254 242 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 390 890 1221
Size: 390 500 331
Fragments arranged by size:
500 390 331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 364 396 1221
Size: 364 32 825
Fragments arranged by size:
825 364 32
XmnI GAAnn'nnTTC
Cuts at: 0 368 1000 1221
Size: 368 632 221
Fragments arranged by size:
632 368 221
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI AvaI AvrII
BamHI BanII BcgI BglII BlpI Bme1580I BplI BpmI
Bpu10I BsaI BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI BtgI BtsI ClaI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NcoI PmlI PspOMI PstI PvuII
SacI SfcI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI BaeI
BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BmgBI BmrI BmtI BpuEI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BstBI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRV FseI FspI FspAI HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI ZraI