(Linear) (Six-base) MAPSORT of: KIBB7969.seq  Check: 3186  from: 1  to: 855



KIBB7969.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:      0     206     855 
   Size:        206     649
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     855 
   Size:        545     310
 
AflIII A'CryG_T

Cuts at:      0     317     855 
   Size:        317     538
 
AlwNI CAG_nnn'CTG

Cuts at:      0     685     855 
   Size:        685     170
 
ApoI r'AATT_y

Cuts at:      0      32      77     157     298     855 
   Size:         32      45      80     141     557

  Fragments arranged by size: 

                 557     141      80      45      32
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     370     855 
   Size:        370     485
 
BfrBI ATG'CAT

Cuts at:      0     826     855 
   Size:        826      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     393     855 
   Size:        393     462
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     454     855 
   Size:        454     401
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     449     855 
   Size:        251     198     406

  Fragments arranged by size: 

                 406     251     198
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     304     334     855 
   Size:        304      30     521

  Fragments arranged by size: 

                 521     304      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     394     855 
   Size:        394     461
 
BsmI GAATG_Cn'

Cuts at:      0     828     855 
   Size:        828      27
 
BspHI T'CATG_A

Cuts at:      0     593     855 
   Size:        593     262
 
BsrDI GCAATG_nn'

Cuts at:      0     174     691     855 
   Size:        174     517     164

  Fragments arranged by size: 

                 517     174     164
 
BsrGI T'GTAC_A

Cuts at:      0     804     855 
   Size:        804      51
 
BstYI r'GATC_y

Cuts at:      0     548     682     855 
   Size:        548     134     173

  Fragments arranged by size: 

                 548     173     134
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     747     782     855 
   Size:        747      35      73

  Fragments arranged by size: 

                 747      73      35
 
DraI TTT'AAA

Cuts at:      0     297     369     855 
   Size:        297      72     486

  Fragments arranged by size: 

                 486     297      72
 
EarI CTCTTCn'nnn_

Cuts at:      0      59     592     827     855 
   Size:         59     533     235      28

  Fragments arranged by size: 

                 533     235      59      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     449     545     855 
   Size:        251     198      96     310

  Fragments arranged by size: 

                 310     251     198      96
 
EcoRI G'AATT_C

Cuts at:      0     157     855 
   Size:        157     698
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     550     855 
   Size:        550     305
 
MslI CAynn'nnrTG

Cuts at:      0     703     749     776     855 
   Size:        703      46      27      79

  Fragments arranged by size: 

                 703      79      46      27
 
MspA1I CmG'CkG

Cuts at:      0       7     855 
   Size:          7     848
 
NdeI CA'TA_TG

Cuts at:      0     828     855 
   Size:        828      27
 
NsiI A_TGCA'T

Cuts at:      0     828     855 
   Size:        828      27
 
NspI r_CATG'y

Cuts at:      0     321     855 
   Size:        321     534
 
PciI A'CATG_T

Cuts at:      0     317     855 
   Size:        317     538
 
PfoI T'CCnGG_A

Cuts at:      0     267     855 
   Size:        267     588
 
SapI GCTCTTCn'nnn_

Cuts at:      0     592     855 
   Size:        592     263
 
ScaI AGT'ACT

Cuts at:      0     403     855 
   Size:        403     452
 
SfcI C'TryA_G

Cuts at:      0      14     335     855 
   Size:         14     321     520

  Fragments arranged by size: 

                 520     321      14
 
SspI AAT'ATT

Cuts at:      0     763     855 
   Size:        763      92
 
StyI C'CwwG_G

Cuts at:      0     783     855 
   Size:        783      72
 
TatI w'GTAC_w

Cuts at:      0     401     804     855 
   Size:        401     403      51

  Fragments arranged by size: 

                 403     401      51
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     111     143     855 
   Size:        111      32     712

  Fragments arranged by size: 

                 712     111      32
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI    BciVI    BfrBI     BmrI
     BplI     BpmI    BsaXI    BseRI     BsmI    BspHI    BsrDI    BsrGI
    BstYI    CspCI     DraI     EarI  Eco57MI    EcoRI     FalI     MslI
   MspA1I     NdeI     NsiI     NspI     PciI     PfoI     SapI     ScaI
     SfcI     SspI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI