Restriction map for
pF1KB7974
(Linear) (Six-base) MAPSORT of: KIBB7974.seq Check: 1305 from: 1 to: 1239
KIBB7974.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 656 1239
Size: 568 88 583
Fragments arranged by size:
583 568 88
AleI CACnn'nnGTG
Cuts at: 0 1080 1239
Size: 1080 159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 920 1239
Size: 920 319
AlwNI CAG_nnn'CTG
Cuts at: 0 796 1239
Size: 796 443
ApaI G_GGCC'C
Cuts at: 0 477 871 1239
Size: 477 394 368
Fragments arranged by size:
477 394 368
AvaI C'yCGr_G
Cuts at: 0 33 269 1158 1239
Size: 33 236 889 81
Fragments arranged by size:
889 236 81 33
BanI G'GyrC_C
Cuts at: 0 21 484 498 1239
Size: 21 463 14 741
Fragments arranged by size:
741 463 21 14
BanII G_rGCy'C
Cuts at: 0 299 477 604 655 851 871 1010 1093
Size: 299 178 127 51 196 20 139 83
Cuts at: 1093 1239
Size: 146
Fragments arranged by size:
299 196 178 146 139 127 83 51
20
BbeI G_GCGC'C
Cuts at: 0 25 1239
Size: 25 1214
BbsI GAAGACnn'nnnn_
Cuts at: 0 893 1239
Size: 893 346
BbvCI CC'TCA_GC
Cuts at: 0 734 1239
Size: 734 505
BglI GCCn_nnn'nGGC
Cuts at: 0 472 1239
Size: 472 767
Bme1580I G_kGCm'C
Cuts at: 0 40 477 503 871 1239
Size: 40 437 26 368 368
Fragments arranged by size:
437 368 368 40 26
BmgBI CAC'GTC
Cuts at: 0 393 721 1239
Size: 393 328 518
Fragments arranged by size:
518 393 328
BmrI ACTGGGnnnn_n'
Cuts at: 0 696 874 1239
Size: 696 178 365
Fragments arranged by size:
696 365 178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 475 1239
Size: 475 764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 514 631 1123 1239
Size: 470 44 117 492 116
Fragments arranged by size:
492 470 117 116 44
Bpu10I CC'TnA_GC
Cuts at: 0 734 1239
Size: 734 505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 283 1239
Size: 112 171 956
Fragments arranged by size:
956 171 112
BsaI GGTCTCn'nnnn_
Cuts at: 0 520 1157 1239
Size: 520 637 82
Fragments arranged by size:
637 520 82
BsaHI Gr'CG_yC
Cuts at: 0 22 1239
Size: 22 1217
BsaWI w'CCGG_w
Cuts at: 0 374 979 1239
Size: 374 605 260
Fragments arranged by size:
605 374 260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1099 1129 1239
Size: 1099 30 110
Fragments arranged by size:
1099 110 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 1120 1239
Size: 328 792 119
Fragments arranged by size:
792 328 119
BseYI C'CCAG_C
Cuts at: 0 789 1239
Size: 789 450
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 415 785 1239
Size: 382 33 370 454
Fragments arranged by size:
454 382 370 33
BsiHKAI G_wGCw'C
Cuts at: 0 299 604 851 1093 1239
Size: 299 305 247 242 146
Fragments arranged by size:
305 299 247 242 146
Bsp1286I G_dGCh'C
Cuts at: 0 40 299 477 503 604 655 851 871
Size: 40 259 178 26 101 51 196 20
Cuts at: 871 1010 1093 1239
Size: 139 83 146
Fragments arranged by size:
259 196 178 146 139 101 83 51
40 26 20
BspEI T'CCGG_A
Cuts at: 0 374 979 1239
Size: 374 605 260
Fragments arranged by size:
605 374 260
BspHI T'CATG_A
Cuts at: 0 423 1239
Size: 423 816
BsrFI r'CCGG_y
Cuts at: 0 47 1239
Size: 47 1192
BssSI C'ACGA_G
Cuts at: 0 1076 1239
Size: 1076 163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 192 1239
Size: 192 1047
BtsI GCAGTG_nn'
Cuts at: 0 746 768 892 1239
Size: 746 22 124 347
Fragments arranged by size:
746 347 124 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 822 857 1239
Size: 822 35 382
Fragments arranged by size:
822 382 35
DraIII CAC_nnn'GTG
Cuts at: 0 354 1239
Size: 354 885
EaeI y'GGCC_r
Cuts at: 0 464 889 1239
Size: 464 425 350
Fragments arranged by size:
464 425 350
EarI CTCTTCn'nnn_
Cuts at: 0 544 1239
Size: 544 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 183 1239
Size: 19 164 1056
Fragments arranged by size:
1056 164 19
EcoICRI GAG'CTC
Cuts at: 0 297 602 849 1091 1239
Size: 297 305 247 242 148
Fragments arranged by size:
305 297 247 242 148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 514 568 631 656 1123 1239
Size: 470 44 54 63 25 467 116
Fragments arranged by size:
470 467 116 63 54 44 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 1239
Size: 97 1142
EcoO109I rG'GnC_Cy
Cuts at: 0 253 474 1239
Size: 253 221 765
Fragments arranged by size:
765 253 221
HaeII r_GCGC'y
Cuts at: 0 25 1239
Size: 25 1214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 1099 1131 1239
Size: 205 32 862 32 108
Fragments arranged by size:
862 205 108 32 32
KasI G'GCGC_C
Cuts at: 0 21 1239
Size: 21 1218
MscI TGG'CCA
Cuts at: 0 466 1239
Size: 466 773
MslI CAynn'nnrTG
Cuts at: 0 1080 1239
Size: 1080 159
MspA1I CmG'CkG
Cuts at: 0 139 327 404 796 1239
Size: 139 188 77 392 443
Fragments arranged by size:
443 392 188 139 77
NaeI GCC'GGC
Cuts at: 0 49 1239
Size: 49 1190
NarI GG'CG_CC
Cuts at: 0 22 1239
Size: 22 1217
NgoMIV G'CCGG_C
Cuts at: 0 47 1239
Size: 47 1192
PasI CC'CwG_GG
Cuts at: 0 820 1239
Size: 820 419
PflMI CCAn_nnn'nTGG
Cuts at: 0 780 1239
Size: 780 459
PspOMI G'GGCC_C
Cuts at: 0 473 867 1239
Size: 473 394 372
Fragments arranged by size:
473 394 372
PstI C_TGCA'G
Cuts at: 0 101 323 439 899 1239
Size: 101 222 116 460 340
Fragments arranged by size:
460 340 222 116 101
PvuII CAG'CTG
Cuts at: 0 139 404 796 1239
Size: 139 265 392 443
Fragments arranged by size:
443 392 265 139
SacI G_AGCT'C
Cuts at: 0 299 604 851 1093 1239
Size: 299 305 247 242 146
Fragments arranged by size:
305 299 247 242 146
SfcI C'TryA_G
Cuts at: 0 97 319 435 895 1239
Size: 97 222 116 460 344
Fragments arranged by size:
460 344 222 116 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 472 1239
Size: 472 767
SfoI GGC'GCC
Cuts at: 0 23 1239
Size: 23 1216
SmaI CCC'GGG
Cuts at: 0 35 271 1160 1239
Size: 35 236 889 79
Fragments arranged by size:
889 236 79 35
SmlI C'TyrA_G
Cuts at: 0 127 298 1239
Size: 127 171 941
Fragments arranged by size:
941 171 127
StyI C'CwwG_G
Cuts at: 0 107 1239
Size: 107 1132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 348 1239
Size: 348 891
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 781 1032 1239
Size: 781 251 207
Fragments arranged by size:
781 251 207
XmaI C'CCGG_G
Cuts at: 0 33 269 1158 1239
Size: 33 236 889 81
Fragments arranged by size:
889 236 81 33
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BsrFI BssSI
BstXI BtsI CspCI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NgoMIV PasI PflMI PspOMI PstI
PvuII SacI SfcI SfiI SfoI SmaI SmlI StyI
TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI
BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DrdI
EagI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI
ZraI