Restriction map for
pF1KB7987
(Linear) (Six-base) MAPSORT of: KIBB7987.seq Check: 1981 from: 1 to: 1323
KIBB7987.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
Acc65I G'GTAC_C
Cuts at: 0 1142 1323
Size: 1142 181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 856 873 931 1060 1323
Size: 154 702 17 58 129 263
Fragments arranged by size:
702 263 154 129 58 17
AhdI GACnn_n'nnGTC
Cuts at: 0 168 321 1323
Size: 168 153 1002
Fragments arranged by size:
1002 168 153
AleI CACnn'nnGTG
Cuts at: 0 1310 1323
Size: 1310 13
AlwNI CAG_nnn'CTG
Cuts at: 0 447 675 692 834 1189 1323
Size: 447 228 17 142 355 134
Fragments arranged by size:
447 355 228 142 134 17
ApaI G_GGCC'C
Cuts at: 0 68 219 907 1323
Size: 68 151 688 416
Fragments arranged by size:
688 416 151 68
ApaLI G'TGCA_C
Cuts at: 0 871 1108 1323
Size: 871 237 215
Fragments arranged by size:
871 237 215
AvaI C'yCGr_G
Cuts at: 0 794 1288 1323
Size: 794 494 35
Fragments arranged by size:
794 494 35
BamHI G'GATC_C
Cuts at: 0 512 848 1025 1323
Size: 512 336 177 298
Fragments arranged by size:
512 336 298 177
BanI G'GyrC_C
Cuts at: 0 1142 1323
Size: 1142 181
BanII G_rGCy'C
Cuts at: 0 68 219 907 1121 1323
Size: 68 151 688 214 202
Fragments arranged by size:
688 214 202 151 68
BglI GCCn_nnn'nGGC
Cuts at: 0 151 1323
Size: 151 1172
Bme1580I G_kGCm'C
Cuts at: 0 68 219 362 460 875 907 1112 1323
Size: 68 151 143 98 415 32 205 211
Fragments arranged by size:
415 211 205 151 143 98 68 32
BmgBI CAC'GTC
Cuts at: 0 141 1323
Size: 141 1182
BmrI ACTGGGnnnn_n'
Cuts at: 0 1092 1323
Size: 1092 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1323
Size: 277 1046
Bpu10I CC'TnA_GC
Cuts at: 0 1115 1323
Size: 1115 208
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 1295 1323
Size: 517 778 28
Fragments arranged by size:
778 517 28
BsaBI GATnn'nnATC
Cuts at: 0 127 1323
Size: 127 1196
BsaHI Gr'CG_yC
Cuts at: 0 41 367 496 1323
Size: 41 326 129 827
Fragments arranged by size:
827 326 129 41
BsaWI w'CCGG_w
Cuts at: 0 287 1323
Size: 287 1036
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 1000 1323
Size: 391 609 323
Fragments arranged by size:
609 391 323
BseYI C'CCAG_C
Cuts at: 0 642 1182 1207 1323
Size: 642 540 25 116
Fragments arranged by size:
642 540 116 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 96 421 688 918 1323
Size: 55 41 325 267 230 405
Fragments arranged by size:
405 325 267 230 55 41
BsiEI CG_ry'CG
Cuts at: 0 329 386 1323
Size: 329 57 937
Fragments arranged by size:
937 329 57
BsiHKAI G_wGCw'C
Cuts at: 0 602 725 875 1112 1121 1323
Size: 602 123 150 237 9 202
Fragments arranged by size:
602 237 202 150 123 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 373 1323
Size: 373 950
Bsp1286I G_dGCh'C
Cuts at: 0 68 219 362 460 602 725 875 907
Size: 68 151 143 98 142 123 150 32
Cuts at: 907 1112 1121 1323
Size: 205 9 202
Fragments arranged by size:
205 202 151 150 143 142 123 98
68 32 9
BspEI T'CCGG_A
Cuts at: 0 287 1323
Size: 287 1036
BspHI T'CATG_A
Cuts at: 0 56 1323
Size: 56 1267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 683 1130 1323
Size: 683 447 193
Fragments arranged by size:
683 447 193
BsrBI CCG'CTC
Cuts at: 0 636 1323
Size: 636 687
BsrDI GCAATG_nn'
Cuts at: 0 91 341 1323
Size: 91 250 982
Fragments arranged by size:
982 250 91
BsrFI r'CCGG_y
Cuts at: 0 419 1323
Size: 419 904
BssSI C'ACGA_G
Cuts at: 0 461 874 1323
Size: 461 413 449
Fragments arranged by size:
461 449 413
BstAPI GCAn_nnn'nTGC
Cuts at: 0 675 1323
Size: 675 648
BstYI r'GATC_y
Cuts at: 0 182 482 512 848 1001 1025 1323
Size: 182 300 30 336 153 24 298
Fragments arranged by size:
336 300 298 182 153 30 24
BtgI C'CryG_G
Cuts at: 0 76 362 516 852 1227 1323
Size: 76 286 154 336 375 96
Fragments arranged by size:
375 336 286 154 96 76
BtsI GCAGTG_nn'
Cuts at: 0 303 569 705 1323
Size: 303 266 136 618
Fragments arranged by size:
618 303 266 136
DrdI GACnn_nn'nnGTC
Cuts at: 0 751 1102 1323
Size: 751 351 221
Fragments arranged by size:
751 351 221
EaeI y'GGCC_r
Cuts at: 0 102 143 326 383 1272 1323
Size: 102 41 183 57 889 51
Fragments arranged by size:
889 183 102 57 51 41
EagI C'GGCC_G
Cuts at: 0 326 383 1323
Size: 326 57 940
Fragments arranged by size:
940 326 57
EarI CTCTTCn'nnn_
Cuts at: 0 607 685 1323
Size: 607 78 638
Fragments arranged by size:
638 607 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1309 1323
Size: 1309 14
EcoICRI GAG'CTC
Cuts at: 0 1119 1323
Size: 1119 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 277 856 873 931 1060 1323
Size: 154 123 579 17 58 129 263
Fragments arranged by size:
579 263 154 129 123 58 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 838 1137 1323
Size: 202 636 299 186
Fragments arranged by size:
636 299 202 186
EcoO109I rG'GnC_Cy
Cuts at: 0 27 65 195 215 216 252 672 773
Size: 27 38 130 20 1 36 420 101
Cuts at: 773 831 903 904 1323
Size: 58 72 1 419
Fragments arranged by size:
420 419 130 101 72 58 38 36
27 20 1 1
HaeII r_GCGC'y
Cuts at: 0 448 506 571 643 823 1214 1323
Size: 448 58 65 72 180 391 109
Fragments arranged by size:
448 391 180 109 72 65 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 38 70 154 186 307 339 481 513
Size: 38 32 84 32 121 32 142 32
Cuts at: 513 952 984 1323
Size: 439 32 339
Fragments arranged by size:
439 339 142 121 84 38 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1053 1323
Size: 1053 270
KpnI G_GTAC'C
Cuts at: 0 1146 1323
Size: 1146 177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 602 860 1323
Size: 69 533 258 463
Fragments arranged by size:
533 463 258 69
MscI TGG'CCA
Cuts at: 0 145 1274 1323
Size: 145 1129 49
Fragments arranged by size:
1129 145 49
MslI CAynn'nnrTG
Cuts at: 0 976 1310 1323
Size: 976 334 13
Fragments arranged by size:
976 334 13
MspA1I CmG'CkG
Cuts at: 0 78 364 518 854 1186 1323
Size: 78 286 154 336 332 137
Fragments arranged by size:
336 332 286 154 137 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 1082 1323
Size: 1082 241
PpuMI rG'GwC_Cy
Cuts at: 0 195 252 672 1323
Size: 195 57 420 651
Fragments arranged by size:
651 420 195 57
PspOMI G'GGCC_C
Cuts at: 0 64 215 903 1323
Size: 64 151 688 420
Fragments arranged by size:
688 420 151 64
PstI C_TGCA'G
Cuts at: 0 653 671 1323
Size: 653 18 652
Fragments arranged by size:
653 652 18
PvuII CAG'CTG
Cuts at: 0 1186 1323
Size: 1186 137
SacI G_AGCT'C
Cuts at: 0 1121 1323
Size: 1121 202
SacII CC_GC'GG
Cuts at: 0 79 365 519 855 1323
Size: 79 286 154 336 468
Fragments arranged by size:
468 336 286 154 79
SapI GCTCTTCn'nnn_
Cuts at: 0 607 685 1323
Size: 607 78 638
Fragments arranged by size:
638 607 78
SbfI CC_TGCA'GG
Cuts at: 0 671 1323
Size: 671 652
SfcI C'TryA_G
Cuts at: 0 649 667 1134 1323
Size: 649 18 467 189
Fragments arranged by size:
649 467 189 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 151 1323
Size: 151 1172
SmaI CCC'GGG
Cuts at: 0 796 1323
Size: 796 527
StuI AGG'CCT
Cuts at: 0 583 1323
Size: 583 740
StyI C'CwwG_G
Cuts at: 0 527 578 1323
Size: 527 51 745
Fragments arranged by size:
745 527 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 936 1093 1323
Size: 936 157 230
Fragments arranged by size:
936 230 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 282 314 986 1018 1323
Size: 282 32 672 32 305
Fragments arranged by size:
672 305 282 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 545 1323
Size: 545 778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 1323
Size: 252 1071
XmaI C'CCGG_G
Cuts at: 0 794 1323
Size: 794 529
Enzymes that do cut:
Acc65I AcuI AhdI AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstYI BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I
PflMI PpuMI PspOMI PstI PvuII SacI SacII SapI
SbfI SfcI SfiI SmaI StuI StyI TaqII TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI BpuEI BsaAI BsaXI BsiWI BsmI
BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XhoI XmnI
ZraI