Restriction map for
pF1KB7992
(Linear) (Six-base) MAPSORT of: KIBB7992.seq Check: 3895 from: 1 to: 1305
KIBB7992.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
Acc65I G'GTAC_C
Cuts at: 0 757 1305
Size: 757 548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 228 707 1059 1305
Size: 103 125 479 352 246
Fragments arranged by size:
479 352 246 125 103
AhdI GACnn_n'nnGTC
Cuts at: 0 272 1305
Size: 272 1033
AlwNI CAG_nnn'CTG
Cuts at: 0 103 354 508 1305
Size: 103 251 154 797
Fragments arranged by size:
797 251 154 103
ApaI G_GGCC'C
Cuts at: 0 29 1305
Size: 29 1276
ApoI r'AATT_y
Cuts at: 0 857 1025 1193 1305
Size: 857 168 168 112
Fragments arranged by size:
857 168 168 112
AvaI C'yCGr_G
Cuts at: 0 278 1305
Size: 278 1027
BanI G'GyrC_C
Cuts at: 0 757 1305
Size: 757 548
BanII G_rGCy'C
Cuts at: 0 29 404 650 1003 1262 1305
Size: 29 375 246 353 259 43
Fragments arranged by size:
375 353 259 246 43 29
BbvCI CC'TCA_GC
Cuts at: 0 322 1305
Size: 322 983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 290 324 1305
Size: 290 34 981
Fragments arranged by size:
981 290 34
BglII A'GATC_T
Cuts at: 0 227 1305
Size: 227 1078
Bme1580I G_kGCm'C
Cuts at: 0 29 1305
Size: 29 1276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 481 574 971 1305
Size: 376 105 93 397 334
Fragments arranged by size:
397 376 334 105 93
Bpu10I CC'TnA_GC
Cuts at: 0 322 1305
Size: 322 983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 1305
Size: 309 996
BsaI GGTCTCn'nnnn_
Cuts at: 0 184 595 1305
Size: 184 411 710
Fragments arranged by size:
710 411 184
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 457 1305
Size: 412 45 848
Fragments arranged by size:
848 412 45
BseYI C'CCAG_C
Cuts at: 0 605 1305
Size: 605 700
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 494 506 1305
Size: 91 403 12 799
Fragments arranged by size:
799 403 91 12
BsiHKAI G_wGCw'C
Cuts at: 0 404 1003 1089 1255 1262 1305
Size: 404 599 86 166 7 43
Fragments arranged by size:
599 404 166 86 43 7
Bsp1286I G_dGCh'C
Cuts at: 0 29 404 650 1003 1089 1255 1262 1305
Size: 29 375 246 353 86 166 7 43
Fragments arranged by size:
375 353 246 166 86 43 29 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 486 528 1305
Size: 486 42 777
Fragments arranged by size:
777 486 42
BsrDI GCAATG_nn'
Cuts at: 0 664 1305
Size: 664 641
BstBI TT'CG_AA
Cuts at: 0 1049 1305
Size: 1049 256
BstYI r'GATC_y
Cuts at: 0 227 350 1305
Size: 227 123 955
Fragments arranged by size:
955 227 123
Bsu36I CC'TnA_GG
Cuts at: 0 395 596 1305
Size: 395 201 709
Fragments arranged by size:
709 395 201
BtgI C'CryG_G
Cuts at: 0 535 1305
Size: 535 770
EarI CTCTTCn'nnn_
Cuts at: 0 161 524 1305
Size: 161 363 781
Fragments arranged by size:
781 363 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 223 1305
Size: 223 1082
EcoICRI GAG'CTC
Cuts at: 0 402 1001 1260 1305
Size: 402 599 259 45
Fragments arranged by size:
599 402 259 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 228 376 481 574 707 971 1059
Size: 103 125 148 105 93 133 264 88
Cuts at: 1059 1305
Size: 246
Fragments arranged by size:
264 246 148 133 125 105 103 93
88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 394 1305
Size: 394 911
EcoO109I rG'GnC_Cy
Cuts at: 0 25 26 498 505 1305
Size: 25 1 472 7 800
Fragments arranged by size:
800 472 25 7 1
EcoRI G'AATT_C
Cuts at: 0 1025 1193 1305
Size: 1025 168 112
Fragments arranged by size:
1025 168 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 258 290 391 423 646 678
Size: 160 32 66 32 101 32 223 32
Cuts at: 678 1305
Size: 627
Fragments arranged by size:
627 223 160 101 66 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1238 1305
Size: 1238 67
KpnI G_GTAC'C
Cuts at: 0 761 1305
Size: 761 544
MspA1I CmG'CkG
Cuts at: 0 44 103 1305
Size: 44 59 1202
Fragments arranged by size:
1202 59 44
NcoI C'CATG_G
Cuts at: 0 535 1305
Size: 535 770
PfoI T'CCnGG_A
Cuts at: 0 353 1305
Size: 353 952
PpuMI rG'GwC_Cy
Cuts at: 0 498 1305
Size: 498 807
PsiI TTA'TAA
Cuts at: 0 914 1305
Size: 914 391
PspOMI G'GGCC_C
Cuts at: 0 25 1305
Size: 25 1280
PstI C_TGCA'G
Cuts at: 0 63 773 1305
Size: 63 710 532
Fragments arranged by size:
710 532 63
PvuII CAG'CTG
Cuts at: 0 44 103 1305
Size: 44 59 1202
Fragments arranged by size:
1202 59 44
SacI G_AGCT'C
Cuts at: 0 404 1003 1262 1305
Size: 404 599 259 43
Fragments arranged by size:
599 404 259 43
SapI GCTCTTCn'nnn_
Cuts at: 0 524 1305
Size: 524 781
ScaI AGT'ACT
Cuts at: 0 305 1305
Size: 305 1000
SfcI C'TryA_G
Cuts at: 0 59 769 1305
Size: 59 710 536
Fragments arranged by size:
710 536 59
SmaI CCC'GGG
Cuts at: 0 280 1305
Size: 280 1025
SmlI C'TyrA_G
Cuts at: 0 288 1305
Size: 288 1017
StuI AGG'CCT
Cuts at: 0 286 1072 1156 1305
Size: 286 786 84 149
Fragments arranged by size:
786 286 149 84
StyI C'CwwG_G
Cuts at: 0 535 650 1305
Size: 535 115 655
Fragments arranged by size:
655 535 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 402 693 1000 1305
Size: 402 291 307 305
Fragments arranged by size:
402 307 305 291
TatI w'GTAC_w
Cuts at: 0 303 1305
Size: 303 1002
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 566 598 1305
Size: 566 32 707
Fragments arranged by size:
707 566 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 268 687 1305
Size: 268 419 618
Fragments arranged by size:
618 419 268
XmaI C'CCGG_G
Cuts at: 0 278 1305
Size: 278 1027
XmnI GAAnn'nnTTC
Cuts at: 0 988 1045 1072 1305
Size: 988 57 27 233
Fragments arranged by size:
988 233 57 27
Enzymes that do cut:
Acc65I AcuI AhdI AlwNI ApaI ApoI AvaI BanI
BanII BbvCI BcgI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BstBI BstYI Bsu36I BtgI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI Hin4I HindIII KpnI MspA1I NcoI
PfoI PpuMI PsiI PspOMI PstI PvuII SacI SapI
ScaI SfcI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI MfeI MluI MmeI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI