(Linear) (Six-base) MAPSORT of: KIBB8003.seq  Check: 7987  from: 1  to: 489



KIBB8003.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     185     489 
   Size:        185     304
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     489 
   Size:        357     132
 
AhdI GACnn_n'nnGTC

Cuts at:      0     309     489 
   Size:        309     180
 
AlwNI CAG_nnn'CTG

Cuts at:      0     335     489 
   Size:        335     154
 
ApaI G_GGCC'C

Cuts at:      0     446     489 
   Size:        446      43
 
BanI G'GyrC_C

Cuts at:      0     367     489 
   Size:        367     122
 
BanII G_rGCy'C

Cuts at:      0     446     489 
   Size:        446      43
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     410     489 
   Size:        410      79
 
BclI T'GATC_A

Cuts at:      0     263     489 
   Size:        263     226
 
BlpI GC'TnA_GC

Cuts at:      0     342     489 
   Size:        342     147
 
Bme1580I G_kGCm'C

Cuts at:      0     446     489 
   Size:        446      43
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     489 
   Size:        277     212
 
Bpu10I CC'TnA_GC

Cuts at:      0      54     489 
   Size:         54     435
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     353     489 
   Size:        353     136
 
BsaHI Gr'CG_yC

Cuts at:      0     407     489 
   Size:        407      82
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     262     313     489 
   Size:         25     237      51     176

  Fragments arranged by size: 

                 237     176      51      25
 
BseYI C'CCAG_C

Cuts at:      0      29     109     489 
   Size:         29      80     380

  Fragments arranged by size: 

                 380      80      29
 
Bsp1286I G_dGCh'C

Cuts at:      0     446     489 
   Size:        446      43
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     185     489 
   Size:        185     304
 
BsrBI CCG'CTC

Cuts at:      0     105     440     489 
   Size:        105     335      49

  Fragments arranged by size: 

                 335     105      49
 
BtsI GCAGTG_nn'

Cuts at:      0     132     489 
   Size:        132     357
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     489 
   Size:         19     470
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     357     489 
   Size:        277      80     132

  Fragments arranged by size: 

                 277     132      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0     252     489 
   Size:        252     237
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     295     325     327     357     489 
   Size:        295      30       2      30     132

  Fragments arranged by size: 

                 295     132      30      30       2
 
MspA1I CmG'CkG

Cuts at:      0     113     167     489 
   Size:        113      54     322

  Fragments arranged by size: 

                 322     113      54
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      98     489 
   Size:         98     391
 
PspOMI G'GGCC_C

Cuts at:      0     442     489 
   Size:        442      47
 
PstI C_TGCA'G

Cuts at:      0     340     489 
   Size:        340     149
 
SfcI C'TryA_G

Cuts at:      0     336     489 
   Size:        336     153
 
StyI C'CwwG_G

Cuts at:      0     242     347     489 
   Size:        242     105     142

  Fragments arranged by size: 

                 242     142     105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     355     489 
   Size:        355     134
 
TatI w'GTAC_w

Cuts at:      0      65     489 
   Size:         65     424
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     360     489 
   Size:        360     129
 

 Enzymes that do cut:

     AarI     AcuI     AhdI    AlwNI     ApaI     BanI    BanII     BbsI
     BclI     BlpI Bme1580I     BpmI   Bpu10I     BsaI    BsaHI    BseRI
    BseYI Bsp1286I    BspMI    BsrBI     BtsI     EciI  Eco57MI EcoO109I
    Hin4I   MspA1I    PflMI   PspOMI     PstI     SfcI     StyI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI