Restriction map for
pF1KB8004
(Linear) (Six-base) MAPSORT of: KIBB8004.seq Check: 3642 from: 1 to: 2112
KIBB8004.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 521 728 2112
Size: 521 207 1384
Fragments arranged by size:
1384 521 207
Acc65I G'GTAC_C
Cuts at: 0 61 1421 2112
Size: 61 1360 691
Fragments arranged by size:
1360 691 61
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 500 557 649 1314 2112
Size: 478 22 57 92 665 798
Fragments arranged by size:
798 665 478 92 57 22
AfeI AGC'GCT
Cuts at: 0 1784 2112
Size: 1784 328
AleI CACnn'nnGTG
Cuts at: 0 1918 2112
Size: 1918 194
AlwNI CAG_nnn'CTG
Cuts at: 0 210 761 1986 2112
Size: 210 551 1225 126
Fragments arranged by size:
1225 551 210 126
ApaI G_GGCC'C
Cuts at: 0 1616 2112
Size: 1616 496
AvaI C'yCGr_G
Cuts at: 0 26 1397 2070 2112
Size: 26 1371 673 42
Fragments arranged by size:
1371 673 42 26
BamHI G'GATC_C
Cuts at: 0 1121 2112
Size: 1121 991
BanI G'GyrC_C
Cuts at: 0 61 420 1421 1959 2112
Size: 61 359 1001 538 153
Fragments arranged by size:
1001 538 359 153 61
BanII G_rGCy'C
Cuts at: 0 1196 1616 1679 1723 1733 2112
Size: 1196 420 63 44 10 379
Fragments arranged by size:
1196 420 379 63 44 10
BbeI G_GCGC'C
Cuts at: 0 1963 2112
Size: 1963 149
BbsI GAAGACnn'nnnn_
Cuts at: 0 956 1402 2112
Size: 956 446 710
Fragments arranged by size:
956 710 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 261 295 628 662 2112
Size: 261 34 333 34 1450
Fragments arranged by size:
1450 333 261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 448 2112
Size: 448 1664
BglI GCCn_nnn'nGGC
Cuts at: 0 1957 1974 2112
Size: 1957 17 138
Fragments arranged by size:
1957 138 17
BglII A'GATC_T
Cuts at: 0 1088 2112
Size: 1088 1024
Bme1580I G_kGCm'C
Cuts at: 0 425 1616 2112
Size: 425 1191 496
Fragments arranged by size:
1191 496 425
BmgBI CAC'GTC
Cuts at: 0 1044 2112
Size: 1044 1068
BmrI ACTGGGnnnn_n'
Cuts at: 0 391 428 534 1210 2112
Size: 391 37 106 676 902
Fragments arranged by size:
902 676 391 106 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 733 1033 1126 2112
Size: 177 556 300 93 986
Fragments arranged by size:
986 556 300 177 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 1132 1735 2112
Size: 276 856 603 377
Fragments arranged by size:
856 603 377 276
BsaI GGTCTCn'nnnn_
Cuts at: 0 1698 2112
Size: 1698 414
BsaBI GATnn'nnATC
Cuts at: 0 391 2112
Size: 391 1721
BsaHI Gr'CG_yC
Cuts at: 0 1517 1960 1966 2112
Size: 1517 443 6 146
Fragments arranged by size:
1517 443 146 6
BsaWI w'CCGG_w
Cuts at: 0 1289 1725 2112
Size: 1289 436 387
Fragments arranged by size:
1289 436 387
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 970 1000 1017 1047 1084 1114
Size: 22 30 918 30 17 30 37 30
Cuts at: 1114 1292 1322 1588 1618 1720 1750 2112
Size: 178 30 266 30 102 30 362
Fragments arranged by size:
918 362 266 178 102 37 30 30
30 30 30 30 30 22 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 1183 1687 2037 2112
Size: 139 1044 504 350 75
Fragments arranged by size:
1044 504 350 139 75
BseYI C'CCAG_C
Cuts at: 0 143 228 424 754 1249 1865 2112
Size: 143 85 196 330 495 616 247
Fragments arranged by size:
616 495 330 247 196 143 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 2112
Size: 1220 892
BsiEI CG_ry'CG
Cuts at: 0 1052 2112
Size: 1052 1060
BsiHKAI G_wGCw'C
Cuts at: 0 1679 1723 1925 2112
Size: 1679 44 202 187
Fragments arranged by size:
1679 202 187 44
BsmI GAATG_Cn'
Cuts at: 0 273 2112
Size: 273 1839
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1050 2112
Size: 1050 1062
Bsp1286I G_dGCh'C
Cuts at: 0 425 1196 1616 1679 1723 1733 1925 2112
Size: 425 771 420 63 44 10 192 187
Fragments arranged by size:
771 425 420 192 187 63 44 10
BspHI T'CATG_A
Cuts at: 0 800 2112
Size: 800 1312
BsrBI CCG'CTC
Cuts at: 0 614 2112
Size: 614 1498
BsrDI GCAATG_nn'
Cuts at: 0 372 2112
Size: 372 1740
BsrFI r'CCGG_y
Cuts at: 0 2000 2112
Size: 2000 112
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2026 2112
Size: 2026 86
BstYI r'GATC_y
Cuts at: 0 736 1088 1121 2112
Size: 736 352 33 991
Fragments arranged by size:
991 736 352 33
Bsu36I CC'TnA_GG
Cuts at: 0 1184 2112
Size: 1184 928
BtgI C'CryG_G
Cuts at: 0 685 1067 1608 1688 2112
Size: 685 382 541 80 424
Fragments arranged by size:
685 541 424 382 80
BtsI GCAGTG_nn'
Cuts at: 0 277 1863 2112
Size: 277 1586 249
Fragments arranged by size:
1586 277 249
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 281 316 2112
Size: 281 35 1796
Fragments arranged by size:
1796 281 35
DraI TTT'AAA
Cuts at: 0 867 2112
Size: 867 1245
DraIII CAC_nnn'GTG
Cuts at: 0 243 1639 2112
Size: 243 1396 473
Fragments arranged by size:
1396 473 243
EaeI y'GGCC_r
Cuts at: 0 2 1049 2112
Size: 2 1047 1063
Fragments arranged by size:
1063 1047 2
EagI C'GGCC_G
Cuts at: 0 1049 2112
Size: 1049 1063
EarI CTCTTCn'nnn_
Cuts at: 0 344 1930 2112
Size: 344 1586 182
Fragments arranged by size:
1586 344 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 1549 2112
Size: 91 1458 563
Fragments arranged by size:
1458 563 91
EcoICRI GAG'CTC
Cuts at: 0 1677 1721 2112
Size: 1677 44 391
Fragments arranged by size:
1677 391 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 478 500 557 649 733 1033 1126
Size: 177 301 22 57 92 84 300 93
Cuts at: 1126 1314 2112
Size: 188 798
Fragments arranged by size:
798 301 300 188 177 93 92 84
57 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 109 121 1383 1450 2112
Size: 109 12 1262 67 662
Fragments arranged by size:
1262 662 109 67 12
EcoO109I rG'GnC_Cy
Cuts at: 0 114 528 568 1179 1497 1613 2086 2112
Size: 114 414 40 611 318 116 473 26
Fragments arranged by size:
611 473 414 318 116 114 40 26
HaeII r_GCGC'y
Cuts at: 0 858 1786 1963 2112
Size: 858 928 177 149
Fragments arranged by size:
928 858 177 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 156 188 721 753 1292 1324
Size: 22 32 102 32 533 32 539 32
Cuts at: 1324 1720 1752 2053 2085 2112
Size: 396 32 301 32 27
Fragments arranged by size:
539 533 396 301 102 32 32 32
32 32 32 27 22
HindIII A'AGCT_T
Cuts at: 0 294 631 2112
Size: 294 337 1481
Fragments arranged by size:
1481 337 294
KasI G'GCGC_C
Cuts at: 0 1959 2112
Size: 1959 153
KpnI G_GTAC'C
Cuts at: 0 65 1425 2112
Size: 65 1360 687
Fragments arranged by size:
1360 687 65
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1440 2112
Size: 1440 672
MscI TGG'CCA
Cuts at: 0 4 2112
Size: 4 2108
MslI CAynn'nnrTG
Cuts at: 0 1792 1918 2112
Size: 1792 126 194
Fragments arranged by size:
1792 194 126
MspA1I CmG'CkG
Cuts at: 0 704 1361 1610 2112
Size: 704 657 249 502
Fragments arranged by size:
704 657 502 249
NarI GG'CG_CC
Cuts at: 0 1960 2112
Size: 1960 152
NcoI C'CATG_G
Cuts at: 0 1688 2112
Size: 1688 424
NspI r_CATG'y
Cuts at: 0 139 1489 2112
Size: 139 1350 623
Fragments arranged by size:
1350 623 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 1639 2112
Size: 1639 473
PfoI T'CCnGG_A
Cuts at: 0 839 901 2112
Size: 839 62 1211
Fragments arranged by size:
1211 839 62
PpuMI rG'GwC_Cy
Cuts at: 0 114 528 2086 2112
Size: 114 414 1558 26
Fragments arranged by size:
1558 414 114 26
PspOMI G'GGCC_C
Cuts at: 0 1612 2112
Size: 1612 500
PstI C_TGCA'G
Cuts at: 0 1084 1469 1982 2112
Size: 1084 385 513 130
Fragments arranged by size:
1084 513 385 130
PvuII CAG'CTG
Cuts at: 0 1361 2112
Size: 1361 751
SacI G_AGCT'C
Cuts at: 0 1679 1723 2112
Size: 1679 44 389
Fragments arranged by size:
1679 389 44
SacII CC_GC'GG
Cuts at: 0 1611 2112
Size: 1611 501
SapI GCTCTTCn'nnn_
Cuts at: 0 344 1930 2112
Size: 344 1586 182
Fragments arranged by size:
1586 344 182
SbfI CC_TGCA'GG
Cuts at: 0 1469 2112
Size: 1469 643
ScaI AGT'ACT
Cuts at: 0 1567 2112
Size: 1567 545
SfcI C'TryA_G
Cuts at: 0 1080 1356 1465 1930 1978 2112
Size: 1080 276 109 465 48 134
Fragments arranged by size:
1080 465 276 134 109 48
SfoI GGC'GCC
Cuts at: 0 1961 2112
Size: 1961 151
SmaI CCC'GGG
Cuts at: 0 28 1399 2112
Size: 28 1371 713
Fragments arranged by size:
1371 713 28
SmlI C'TyrA_G
Cuts at: 0 291 1147 1714 2112
Size: 291 856 567 398
Fragments arranged by size:
856 567 398 291
SphI G_CATG'C
Cuts at: 0 139 2112
Size: 139 1973
StyI C'CwwG_G
Cuts at: 0 989 1058 1688 2057 2112
Size: 989 69 630 369 55
Fragments arranged by size:
989 630 369 69 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 420 2112
Size: 420 1692
TatI w'GTAC_w
Cuts at: 0 1205 1565 2112
Size: 1205 360 547
Fragments arranged by size:
1205 547 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 970 1002 1017 1049 2112
Size: 970 32 15 32 1063
Fragments arranged by size:
1063 970 32 32 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1065 1251 1510 2112
Size: 1065 186 259 602
Fragments arranged by size:
1065 602 259 186
XmaI C'CCGG_G
Cuts at: 0 26 1397 2112
Size: 26 1371 715
Fragments arranged by size:
1371 715 26
Enzymes that do cut:
AccI Acc65I AcuI AfeI AleI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BglI
BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BstXI
BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NspI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SacII SapI SbfI ScaI SfcI SfoI
SmaI SmlI SphI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BclI BfrBI BlpI BmtI BplI Bpu10I BsaAI
BsiWI BspEI BspMI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI DrdI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmnI ZraI