Restriction map for
pF1KB8011
(Linear) (Six-base) MAPSORT of: KIBB8011.seq Check: 593 from: 1 to: 1242
KIBB8011.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 813 1242
Size: 602 211 429
Fragments arranged by size:
602 429 211
AfeI AGC'GCT
Cuts at: 0 8 1242
Size: 8 1234
AflIII A'CryG_T
Cuts at: 0 447 1242
Size: 447 795
AleI CACnn'nnGTG
Cuts at: 0 1075 1242
Size: 1075 167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 593 1242
Size: 593 649
AlwNI CAG_nnn'CTG
Cuts at: 0 754 1242
Size: 754 488
ApaLI G'TGCA_C
Cuts at: 0 443 1242
Size: 443 799
ApoI r'AATT_y
Cuts at: 0 344 1242
Size: 344 898
BbsI GAAGACnn'nnnn_
Cuts at: 0 127 1242
Size: 127 1115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 942 976 1242
Size: 942 34 266
Fragments arranged by size:
942 266 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1238 1242
Size: 1238 4
BlpI GC'TnA_GC
Cuts at: 0 747 1198 1242
Size: 747 451 44
Fragments arranged by size:
747 451 44
Bme1580I G_kGCm'C
Cuts at: 0 447 707 1242
Size: 447 260 535
Fragments arranged by size:
535 447 260
BmgBI CAC'GTC
Cuts at: 0 950 1242
Size: 950 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 410 672 694 712 1123 1242
Size: 334 76 262 22 18 411 119
Fragments arranged by size:
411 334 262 119 76 22 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 670 777 1242
Size: 670 107 465
Fragments arranged by size:
670 465 107
BsaBI GATnn'nnATC
Cuts at: 0 287 1242
Size: 287 955
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 130 715 765 1242
Size: 82 48 585 50 477
Fragments arranged by size:
585 477 82 50 48
BseYI C'CCAG_C
Cuts at: 0 62 89 1242
Size: 62 27 1153
Fragments arranged by size:
1153 62 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 904 1242
Size: 589 315 338
Fragments arranged by size:
589 338 315
BsiHKAI G_wGCw'C
Cuts at: 0 447 1242
Size: 447 795
BsmI GAATG_Cn'
Cuts at: 0 586 1242
Size: 586 656
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1168 1242
Size: 1168 74
Bsp1286I G_dGCh'C
Cuts at: 0 447 707 1242
Size: 447 260 535
Fragments arranged by size:
535 447 260
BsrBI CCG'CTC
Cuts at: 0 746 1242
Size: 746 496
BsrGI T'GTAC_A
Cuts at: 0 1010 1242
Size: 1010 232
BstAPI GCAn_nnn'nTGC
Cuts at: 0 640 1242
Size: 640 602
BstBI TT'CG_AA
Cuts at: 0 413 1242
Size: 413 829
BstXI CCAn_nnnn'nTGG
Cuts at: 0 185 255 339 1242
Size: 185 70 84 903
Fragments arranged by size:
903 185 84 70
BtgI C'CryG_G
Cuts at: 0 338 438 1242
Size: 338 100 804
Fragments arranged by size:
804 338 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 880 979 1242
Size: 880 99 263
Fragments arranged by size:
880 263 99
BtsI GCAGTG_nn'
Cuts at: 0 332 497 965 1015 1242
Size: 332 165 468 50 227
Fragments arranged by size:
468 332 227 165 50
ClaI AT'CG_AT
Cuts at: 0 789 1242
Size: 789 453
EarI CTCTTCn'nnn_
Cuts at: 0 614 1166 1242
Size: 614 552 76
Fragments arranged by size:
614 552 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 410 602 672 694 712 813 1123
Size: 334 76 192 70 22 18 101 310
Cuts at: 1123 1242
Size: 119
Fragments arranged by size:
334 310 192 119 101 76 70 22
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1032 1242
Size: 1032 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1240 1242
Size: 1240 2
HaeII r_GCGC'y
Cuts at: 0 10 1198 1242
Size: 10 1188 44
Fragments arranged by size:
1188 44 10
HincII GTy'rAC
Cuts at: 0 228 1242
Size: 228 1014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 868 935 1010 1242
Size: 316 552 67 75 232
Fragments arranged by size:
552 316 232 75 67
MslI CAynn'nnrTG
Cuts at: 0 183 337 1075 1242
Size: 183 154 738 167
Fragments arranged by size:
738 183 167 154
MspA1I CmG'CkG
Cuts at: 0 1001 1142 1242
Size: 1001 141 100
Fragments arranged by size:
1001 141 100
NcoI C'CATG_G
Cuts at: 0 338 438 1242
Size: 338 100 804
Fragments arranged by size:
804 338 100
NspI r_CATG'y
Cuts at: 0 451 1149 1242
Size: 451 698 93
Fragments arranged by size:
698 451 93
PciI A'CATG_T
Cuts at: 0 447 1242
Size: 447 795
PflMI CCAn_nnn'nTGG
Cuts at: 0 145 1242
Size: 145 1097
PfoI T'CCnGG_A
Cuts at: 0 728 966 1242
Size: 728 238 276
Fragments arranged by size:
728 276 238
PshAI GACnn'nnGTC
Cuts at: 0 830 1242
Size: 830 412
PsiI TTA'TAA
Cuts at: 0 878 1242
Size: 878 364
PstI C_TGCA'G
Cuts at: 0 466 1242
Size: 466 776
PvuII CAG'CTG
Cuts at: 0 1001 1242
Size: 1001 241
SbfI CC_TGCA'GG
Cuts at: 0 466 1242
Size: 466 776
SfcI C'TryA_G
Cuts at: 0 462 1242
Size: 462 780
SphI G_CATG'C
Cuts at: 0 1149 1242
Size: 1149 93
StuI AGG'CCT
Cuts at: 0 1038 1242
Size: 1038 204
StyI C'CwwG_G
Cuts at: 0 80 338 438 524 812 1028 1242
Size: 80 258 100 86 288 216 214
Fragments arranged by size:
288 258 216 214 100 86 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 996 1242
Size: 996 246
TatI w'GTAC_w
Cuts at: 0 980 1010 1079 1121 1242
Size: 980 30 69 42 121
Fragments arranged by size:
980 121 69 42 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 724 756 1242
Size: 724 32 486
Fragments arranged by size:
724 486 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 669 1222 1242
Size: 669 553 20
Fragments arranged by size:
669 553 20
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AlwNI ApaLI ApoI
BbsI BcgI BglI BlpI Bme1580I BmgBI BpmI BsaI
BsaBI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BsrBI BsrGI BstAPI BstBI BstXI BtgI BtgZI BtsI
ClaI EarI Eco57MI EcoNI FalI HaeII HincII MmeI
MslI MspA1I NcoI NspI PciI PflMI PfoI PshAI
PsiI PstI PvuII SbfI SfcI SphI StuI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglII BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BsiEI BsiWI BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI BstEII BstYI BstZ17I Bsu36I CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI