Restriction map for
pF1KB8017
(Linear) (Six-base) MAPSORT of: KIBB8017.seq Check: 5703 from: 1 to: 1941
KIBB8017.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 482 1850 1941
Size: 482 1368 91
Fragments arranged by size:
1368 482 91
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 325 1941
Size: 132 193 1616
Fragments arranged by size:
1616 193 132
AfeI AGC'GCT
Cuts at: 0 1516 1941
Size: 1516 425
AflIII A'CryG_T
Cuts at: 0 337 1941
Size: 337 1604
AhdI GACnn_n'nnGTC
Cuts at: 0 535 1941
Size: 535 1406
AleI CACnn'nnGTG
Cuts at: 0 376 493 1525 1941
Size: 376 117 1032 416
Fragments arranged by size:
1032 416 376 117
AlwNI CAG_nnn'CTG
Cuts at: 0 120 450 1648 1941
Size: 120 330 1198 293
Fragments arranged by size:
1198 330 293 120
ApaI G_GGCC'C
Cuts at: 0 234 1855 1941
Size: 234 1621 86
Fragments arranged by size:
1621 234 86
ApoI r'AATT_y
Cuts at: 0 642 1941
Size: 642 1299
AvaI C'yCGr_G
Cuts at: 0 316 760 1728 1771 1941
Size: 316 444 968 43 170
Fragments arranged by size:
968 444 316 170 43
BamHI G'GATC_C
Cuts at: 0 612 1941
Size: 612 1329
BanI G'GyrC_C
Cuts at: 0 574 1548 1941
Size: 574 974 393
Fragments arranged by size:
974 574 393
BanII G_rGCy'C
Cuts at: 0 234 631 823 928 1855 1941
Size: 234 397 192 105 927 86
Fragments arranged by size:
927 397 234 192 105 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 1471 1941
Size: 1471 470
BclI T'GATC_A
Cuts at: 0 1559 1941
Size: 1559 382
BglI GCCn_nnn'nGGC
Cuts at: 0 566 1941
Size: 566 1375
Bme1580I G_kGCm'C
Cuts at: 0 234 407 704 1855 1941
Size: 234 173 297 1151 86
Fragments arranged by size:
1151 297 234 173 86
BmgBI CAC'GTC
Cuts at: 0 654 1941
Size: 654 1287
BmrI ACTGGGnnnn_n'
Cuts at: 0 237 793 931 1858 1941
Size: 237 556 138 927 83
Fragments arranged by size:
927 556 237 138 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 97 733 1831 1882 1941
Size: 48 49 636 1098 51 59
Fragments arranged by size:
1098 636 59 51 49 48
Bpu10I CC'TnA_GC
Cuts at: 0 817 1941
Size: 817 1124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 1129 1174 1477 1941
Size: 900 229 45 303 464
Fragments arranged by size:
900 464 303 229 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 653 799 908 1120 1370 1807 1882 1941
Size: 653 146 109 212 250 437 75 59
Fragments arranged by size:
653 437 250 212 146 109 75 59
BsaAI yAC'GTr
Cuts at: 0 338 1941
Size: 338 1603
BsaWI w'CCGG_w
Cuts at: 0 662 1112 1941
Size: 662 450 829
Fragments arranged by size:
829 662 450
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 292 322 1339 1369 1399 1941
Size: 292 30 1017 30 30 542
Fragments arranged by size:
1017 542 292 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 897 1138 1941
Size: 897 241 803
Fragments arranged by size:
897 803 241
BseYI C'CCAG_C
Cuts at: 0 295 504 795 838 938 1032 1941
Size: 295 209 291 43 100 94 909
Fragments arranged by size:
909 295 291 209 100 94 43
BsiEI CG_ry'CG
Cuts at: 0 1006 1569 1941
Size: 1006 563 372
Fragments arranged by size:
1006 563 372
BsiHKAI G_wGCw'C
Cuts at: 0 262 386 584 823 1190 1208 1941
Size: 262 124 198 239 367 18 733
Fragments arranged by size:
733 367 262 239 198 124 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1453 1941
Size: 1453 488
Bsp1286I G_dGCh'C
Cuts at: 0 234 262 386 407 584 631 704 823
Size: 234 28 124 21 177 47 73 119
Cuts at: 823 928 1190 1208 1855 1941
Size: 105 262 18 647 86
Fragments arranged by size:
647 262 234 177 124 119 105 86
73 47 28 21 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 728 1664 1850 1941
Size: 482 246 936 186 91
Fragments arranged by size:
936 482 246 186 91
BsrBI CCG'CTC
Cuts at: 0 1910 1941
Size: 1910 31
BsrFI r'CCGG_y
Cuts at: 0 983 1834 1895 1941
Size: 983 851 61 46
Fragments arranged by size:
983 851 61 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 450 1043 1941
Size: 450 593 898
Fragments arranged by size:
898 593 450
BstEII G'GTnAC_C
Cuts at: 0 426 1941
Size: 426 1515
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 1941
Size: 282 1659
BstYI r'GATC_y
Cuts at: 0 92 446 612 665 1941
Size: 92 354 166 53 1276
Fragments arranged by size:
1276 354 166 92 53
Bsu36I CC'TnA_GG
Cuts at: 0 1475 1928 1941
Size: 1475 453 13
Fragments arranged by size:
1475 453 13
BtgI C'CryG_G
Cuts at: 0 252 789 1020 1297 1523 1667 1941
Size: 252 537 231 277 226 144 274
Fragments arranged by size:
537 277 274 252 231 226 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1179 1314 1941
Size: 1179 135 627
Fragments arranged by size:
1179 627 135
BtsI GCAGTG_nn'
Cuts at: 0 171 405 1941
Size: 171 234 1536
Fragments arranged by size:
1536 234 171
DraIII CAC_nnn'GTG
Cuts at: 0 1075 1941
Size: 1075 866
EaeI y'GGCC_r
Cuts at: 0 84 102 985 1003 1050 1566 1832 1836
Size: 84 18 883 18 47 516 266 4
Cuts at: 1836 1941
Size: 105
Fragments arranged by size:
883 516 266 105 84 47 18 18
4
EagI C'GGCC_G
Cuts at: 0 1003 1566 1941
Size: 1003 563 375
Fragments arranged by size:
1003 563 375
EarI CTCTTCn'nnn_
Cuts at: 0 214 1771 1941
Size: 214 1557 170
Fragments arranged by size:
1557 214 170
EcoICRI GAG'CTC
Cuts at: 0 821 1941
Size: 821 1120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 97 132 325 733 1831 1882 1941
Size: 48 49 35 193 408 1098 51 59
Fragments arranged by size:
1098 408 193 59 51 49 48 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1147 1941
Size: 1147 794
EcoO109I rG'GnC_Cy
Cuts at: 0 117 344 799 948 1479 1932 1941
Size: 117 227 455 149 531 453 9
Fragments arranged by size:
531 455 453 227 149 117 9
EcoRI G'AATT_C
Cuts at: 0 642 1941
Size: 642 1299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1062 1941
Size: 1062 879
FseI GG_CCGG'CC
Cuts at: 0 1838 1941
Size: 1838 103
HaeII r_GCGC'y
Cuts at: 0 914 1518 1941
Size: 914 604 423
Fragments arranged by size:
914 604 423
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 521 553 650 682 1318 1350 1369 1401
Size: 521 32 97 32 636 32 19 32
Cuts at: 1401 1582 1614 1941
Size: 181 32 327
Fragments arranged by size:
636 521 327 181 97 32 32 32
32 32 19
HincII GTy'rAC
Cuts at: 0 8 1941
Size: 8 1933
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 989 1941
Size: 636 353 952
Fragments arranged by size:
952 636 353
MscI TGG'CCA
Cuts at: 0 104 1941
Size: 104 1837
MslI CAynn'nnrTG
Cuts at: 0 376 493 1201 1525 1747 1941
Size: 376 117 708 324 222 194
Fragments arranged by size:
708 376 324 222 194 117
MspA1I CmG'CkG
Cuts at: 0 753 842 1860 1869 1941
Size: 753 89 1018 9 72
Fragments arranged by size:
1018 753 89 72 9
NaeI GCC'GGC
Cuts at: 0 985 1836 1941
Size: 985 851 105
Fragments arranged by size:
985 851 105
NcoI C'CATG_G
Cuts at: 0 252 1297 1667 1941
Size: 252 1045 370 274
Fragments arranged by size:
1045 370 274 252
NgoMIV G'CCGG_C
Cuts at: 0 983 1834 1941
Size: 983 851 107
Fragments arranged by size:
983 851 107
NspI r_CATG'y
Cuts at: 0 1827 1941
Size: 1827 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 423 609 795 1941
Size: 423 186 186 1146
Fragments arranged by size:
1146 423 186 186
PfoI T'CCnGG_A
Cuts at: 0 1697 1941
Size: 1697 244
PmlI CAC'GTG
Cuts at: 0 338 1941
Size: 338 1603
PpuMI rG'GwC_Cy
Cuts at: 0 344 948 1932 1941
Size: 344 604 984 9
Fragments arranged by size:
984 604 344 9
PshAI GACnn'nnGTC
Cuts at: 0 433 953 1941
Size: 433 520 988
Fragments arranged by size:
988 520 433
PspOMI G'GGCC_C
Cuts at: 0 230 1851 1941
Size: 230 1621 90
Fragments arranged by size:
1621 230 90
PspXI vC'TCGA_Gb
Cuts at: 0 760 1941
Size: 760 1181
PstI C_TGCA'G
Cuts at: 0 166 892 1941
Size: 166 726 1049
Fragments arranged by size:
1049 726 166
PvuII CAG'CTG
Cuts at: 0 753 1860 1869 1941
Size: 753 1107 9 72
Fragments arranged by size:
1107 753 72 9
SacI G_AGCT'C
Cuts at: 0 823 1941
Size: 823 1118
SapI GCTCTTCn'nnn_
Cuts at: 0 214 1941
Size: 214 1727
ScaI AGT'ACT
Cuts at: 0 187 1941
Size: 187 1754
SfcI C'TryA_G
Cuts at: 0 162 888 1941
Size: 162 726 1053
Fragments arranged by size:
1053 726 162
SmlI C'TyrA_G
Cuts at: 0 760 879 1144 1189 1492 1941
Size: 760 119 265 45 303 449
Fragments arranged by size:
760 449 303 265 119 45
SphI G_CATG'C
Cuts at: 0 1827 1941
Size: 1827 114
StyI C'CwwG_G
Cuts at: 0 252 275 1297 1322 1667 1941
Size: 252 23 1022 25 345 274
Fragments arranged by size:
1022 345 274 252 25 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 245 1941
Size: 245 1696
TatI w'GTAC_w
Cuts at: 0 185 395 1241 1941
Size: 185 210 846 700
Fragments arranged by size:
846 700 210 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 1941
Size: 578 32 1331
Fragments arranged by size:
1331 578 32
XhoI C'TCGA_G
Cuts at: 0 760 1941
Size: 760 1181
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbsI BclI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SapI ScaI SfcI SmlI SphI
StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BcgI BciVI BfrBI BglII BlpI BmtI BplI BsaBI
BsaHI BsgI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstBI BstZ17I ClaI CspCI DraI DrdI
EciI EcoRV FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PpiI PsiI PsrI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XmaI XmnI ZraI