Restriction map for
pF1KB8020
(Linear) (Six-base) MAPSORT of: KIBB8020.seq Check: 5283 from: 1 to: 2070
KIBB8020.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
Acc65I G'GTAC_C
Cuts at: 0 514 2070
Size: 514 1556
AclI AA'CG_TT
Cuts at: 0 375 2070
Size: 375 1695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 835 1204 2070
Size: 335 500 369 866
Fragments arranged by size:
866 500 369 335
AfeI AGC'GCT
Cuts at: 0 642 2070
Size: 642 1428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 943 2070
Size: 943 1127
AlwNI CAG_nnn'CTG
Cuts at: 0 559 2070
Size: 559 1511
ApaI G_GGCC'C
Cuts at: 0 494 2070
Size: 494 1576
ApoI r'AATT_y
Cuts at: 0 704 1242 2070
Size: 704 538 828
Fragments arranged by size:
828 704 538
AvaI C'yCGr_G
Cuts at: 0 896 2070
Size: 896 1174
BanI G'GyrC_C
Cuts at: 0 514 2070
Size: 514 1556
BanII G_rGCy'C
Cuts at: 0 494 2070
Size: 494 1576
BlpI GC'TnA_GC
Cuts at: 0 1141 2070
Size: 1141 929
Bme1580I G_kGCm'C
Cuts at: 0 494 1178 2070
Size: 494 684 892
Fragments arranged by size:
892 684 494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 434 460 509 665 857 2070
Size: 406 28 26 49 156 192 1213
Fragments arranged by size:
1213 406 192 156 49 28 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1678 2070
Size: 449 1229 392
Fragments arranged by size:
1229 449 392
BsaI GGTCTCn'nnnn_
Cuts at: 0 833 2070
Size: 833 1237
BsaWI w'CCGG_w
Cuts at: 0 260 1197 2070
Size: 260 937 873
Fragments arranged by size:
937 873 260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 507 537 1223 1253 2070
Size: 507 30 686 30 817
Fragments arranged by size:
817 686 507 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 36 619 649 1690 1711 2070
Size: 33 3 583 30 1041 21 359
Fragments arranged by size:
1041 583 359 33 30 21 3
BseYI C'CCAG_C
Cuts at: 0 911 1271 1450 1774 2070
Size: 911 360 179 324 296
Fragments arranged by size:
911 360 324 296 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1979 2070
Size: 1979 91
BsiHKAI G_wGCw'C
Cuts at: 0 972 2070
Size: 972 1098
BsmBI CGTCTCn'nnnn_
Cuts at: 0 590 812 2070
Size: 590 222 1258
Fragments arranged by size:
1258 590 222
Bsp1286I G_dGCh'C
Cuts at: 0 494 972 1178 2070
Size: 494 478 206 892
Fragments arranged by size:
892 494 478 206
BspEI T'CCGG_A
Cuts at: 0 1197 2070
Size: 1197 873
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 502 2070
Size: 502 1568
BsrBI CCG'CTC
Cuts at: 0 567 2070
Size: 567 1503
BsrDI GCAATG_nn'
Cuts at: 0 809 882 1155 1616 2070
Size: 809 73 273 461 454
Fragments arranged by size:
809 461 454 273 73
BsrGI T'GTAC_A
Cuts at: 0 110 2070
Size: 110 1960
BssSI C'ACGA_G
Cuts at: 0 998 1523 2070
Size: 998 525 547
Fragments arranged by size:
998 547 525
BstYI r'GATC_y
Cuts at: 0 504 1368 2070
Size: 504 864 702
Fragments arranged by size:
864 702 504
BtgI C'CryG_G
Cuts at: 0 1509 1976 2070
Size: 1509 467 94
Fragments arranged by size:
1509 467 94
BtsI GCAGTG_nn'
Cuts at: 0 560 925 2070
Size: 560 365 1145
Fragments arranged by size:
1145 560 365
DraIII CAC_nnn'GTG
Cuts at: 0 193 2070
Size: 193 1877
DrdI GACnn_nn'nnGTC
Cuts at: 0 582 2070
Size: 582 1488
EaeI y'GGCC_r
Cuts at: 0 78 1163 1955 2070
Size: 78 1085 792 115
Fragments arranged by size:
1085 792 115 78
EarI CTCTTCn'nnn_
Cuts at: 0 54 593 998 1028 1853 2070
Size: 54 539 405 30 825 217
Fragments arranged by size:
825 539 405 217 54 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 406 434 460 509 665 835 857
Size: 335 71 28 26 49 156 170 22
Cuts at: 857 1204 2070
Size: 347 866
Fragments arranged by size:
866 347 335 170 156 71 49 28
26 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1039 2070
Size: 1039 1031
HaeII r_GCGC'y
Cuts at: 0 644 957 2070
Size: 644 313 1113
Fragments arranged by size:
1113 644 313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 976 1008 2070
Size: 10 32 934 32 1062
Fragments arranged by size:
1062 934 32 32 10
HincII GTy'rAC
Cuts at: 0 858 1973 2070
Size: 858 1115 97
Fragments arranged by size:
1115 858 97
KpnI G_GTAC'C
Cuts at: 0 518 2070
Size: 518 1552
MfeI C'AATT_G
Cuts at: 0 1250 2070
Size: 1250 820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1088 2070
Size: 236 852 982
Fragments arranged by size:
982 852 236
MscI TGG'CCA
Cuts at: 0 80 1165 2070
Size: 80 1085 905
Fragments arranged by size:
1085 905 80
MslI CAynn'nnrTG
Cuts at: 0 890 1489 1951 2070
Size: 890 599 462 119
Fragments arranged by size:
890 599 462 119
NcoI C'CATG_G
Cuts at: 0 1509 2070
Size: 1509 561
NspI r_CATG'y
Cuts at: 0 1391 2070
Size: 1391 679
PflMI CCAn_nnn'nTGG
Cuts at: 0 1510 2070
Size: 1510 560
PfoI T'CCnGG_A
Cuts at: 0 411 785 2070
Size: 411 374 1285
Fragments arranged by size:
1285 411 374
PspOMI G'GGCC_C
Cuts at: 0 490 2070
Size: 490 1580
PstI C_TGCA'G
Cuts at: 0 176 513 2070
Size: 176 337 1557
Fragments arranged by size:
1557 337 176
SapI GCTCTTCn'nnn_
Cuts at: 0 1028 2070
Size: 1028 1042
SbfI CC_TGCA'GG
Cuts at: 0 513 2070
Size: 513 1557
ScaI AGT'ACT
Cuts at: 0 1710 2070
Size: 1710 360
SfcI C'TryA_G
Cuts at: 0 172 509 2070
Size: 172 337 1561
Fragments arranged by size:
1561 337 172
SmlI C'TyrA_G
Cuts at: 0 464 1657 2070
Size: 464 1193 413
Fragments arranged by size:
1193 464 413
SpeI A'CTAG_T
Cuts at: 0 454 1998 2070
Size: 454 1544 72
Fragments arranged by size:
1544 454 72
StyI C'CwwG_G
Cuts at: 0 1434 1509 2070
Size: 1434 75 561
Fragments arranged by size:
1434 561 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 701 739 1169 2070
Size: 701 38 430 901
Fragments arranged by size:
901 701 430 38
TatI w'GTAC_w
Cuts at: 0 110 1708 2070
Size: 110 1598 362
Fragments arranged by size:
1598 362 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1223 1255 1646 1678 2070
Size: 1223 32 391 32 392
Fragments arranged by size:
1223 392 391 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 142 2070
Size: 142 1928
XmnI GAAnn'nnTTC
Cuts at: 0 244 2070
Size: 244 1826
Enzymes that do cut:
Acc65I AclI AcuI AfeI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BlpI Bme1580I BpmI BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrGI BssSI BstYI BtgI
BtsI DraIII DrdI EaeI EarI Eco57MI FalI HaeII
Hin4I HincII KpnI MfeI MmeI MscI MslI NcoI
NspI PflMI PfoI PspOMI PstI SapI SbfI ScaI
SfcI SmlI SpeI StyI TaqII TatI TstI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII AccI AflII AflIII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsiEI BsiWI BsmI BspHI BsrFI BssHII BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI MluI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI StuI SwaI XbaI XcmI
XhoI XmaI ZraI