(Linear) (Six-base) MAPSORT of: KIBB8103.seq  Check: 7630  from: 1  to: 234



KIBB8103.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

BanI G'GyrC_C

Cuts at:      0     109     219     234 
   Size:        109     110      15

  Fragments arranged by size: 

                 110     109      15
 
BanII G_rGCy'C

Cuts at:      0      40     234 
   Size:         40     194
 
BbeI G_GCGC'C

Cuts at:      0     113     234 
   Size:        113     121
 
BglI GCCn_nnn'nGGC

Cuts at:      0      44     149     234 
   Size:         44     105      85

  Fragments arranged by size: 

                 105      85      44
 
BglII A'GATC_T

Cuts at:      0      68     234 
   Size:         68     166
 
BlpI GC'TnA_GC

Cuts at:      0      34     234 
   Size:         34     200
 
BmgBI CAC'GTC

Cuts at:      0      60     195     234 
   Size:         60     135      39

  Fragments arranged by size: 

                 135      60      39
 
BsaHI Gr'CG_yC

Cuts at:      0     110     234 
   Size:        110     124
 
BseYI C'CCAG_C

Cuts at:      0     138     234 
   Size:        138      96
 
BsiEI CG_ry'CG

Cuts at:      0     130     153     234 
   Size:        130      23      81

  Fragments arranged by size: 

                 130      81      23
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     234 
   Size:         40     194
 
BstYI r'GATC_y

Cuts at:      0      68     234 
   Size:         68     166
 
EaeI y'GGCC_r

Cuts at:      0     127     150     234 
   Size:        127      23      84

  Fragments arranged by size: 

                 127      84      23
 
EagI C'GGCC_G

Cuts at:      0     127     150     234 
   Size:        127      23      84

  Fragments arranged by size: 

                 127      84      23
 
HaeII r_GCGC'y

Cuts at:      0     113     234 
   Size:        113     121
 
KasI G'GCGC_C

Cuts at:      0     109     234 
   Size:        109     125
 
NarI GG'CG_CC

Cuts at:      0     110     234 
   Size:        110     124
 
NotI GC'GGCC_GC

Cuts at:      0     127     234 
   Size:        127     107
 
PstI C_TGCA'G

Cuts at:      0      89     234 
   Size:         89     145
 
SbfI CC_TGCA'GG

Cuts at:      0      89     234 
   Size:         89     145
 
SfcI C'TryA_G

Cuts at:      0      85     234 
   Size:         85     149
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     149     234 
   Size:        149      85
 
SfoI GGC'GCC

Cuts at:      0     111     234 
   Size:        111     123
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226     234 
   Size:        226       8
 

 Enzymes that do cut:

     BanI    BanII     BbeI     BglI    BglII     BlpI    BmgBI    BsaHI
    BseYI    BsiEI Bsp1286I    BstYI     EaeI     EagI    HaeII     KasI
     NarI     NotI     PstI     SbfI     SfcI     SfiI     SfoI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI