(Linear) (Six-base) MAPSORT of: KIBB8107.seq  Check: 6190  from: 1  to: 666



KIBB8107.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AleI CACnn'nnGTG

Cuts at:      0     325     666 
   Size:        325     341
 
ApoI r'AATT_y

Cuts at:      0     500     666 
   Size:        500     166
 
BclI T'GATC_A

Cuts at:      0     200     666 
   Size:        200     466
 
BfrBI ATG'CAT

Cuts at:      0     235     666 
   Size:        235     431
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     536     666 
   Size:        536     130
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     563     649     666 
   Size:        563      86      17

  Fragments arranged by size: 

                 563      86      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     436     622     625     628     666 
   Size:        436     186       3       3      38

  Fragments arranged by size: 

                 436     186      38       3       3
 
BseYI C'CCAG_C

Cuts at:      0     409     666 
   Size:        409     257
 
BsiHKAI G_wGCw'C

Cuts at:      0     263     322     666 
   Size:        263      59     344

  Fragments arranged by size: 

                 344     263      59
 
Bsp1286I G_dGCh'C

Cuts at:      0     263     322     666 
   Size:        263      59     344

  Fragments arranged by size: 

                 344     263      59
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      42     666 
   Size:         42     624
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     601     666 
   Size:        601      65
 
EaeI y'GGCC_r

Cuts at:      0     188     650     666 
   Size:        188     462      16

  Fragments arranged by size: 

                 462     188      16
 
EarI CTCTTCn'nnn_

Cuts at:      0     310     614     666 
   Size:        310     304      52

  Fragments arranged by size: 

                 310     304      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     563     649     666 
   Size:        563      86      17

  Fragments arranged by size: 

                 563      86      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0     524     666 
   Size:        524     142
 
EcoRI G'AATT_C

Cuts at:      0     500     666 
   Size:        500     166
 
EcoRV GAT'ATC

Cuts at:      0     108     666 
   Size:        108     558
 
HincII GTy'rAC

Cuts at:      0      90     666 
   Size:         90     576
 
MscI TGG'CCA

Cuts at:      0     190     652     666 
   Size:        190     462      14

  Fragments arranged by size: 

                 462     190      14
 
MslI CAynn'nnrTG

Cuts at:      0     268     325     666 
   Size:        268      57     341

  Fragments arranged by size: 

                 341     268      57
 
NsiI A_TGCA'T

Cuts at:      0     237     666 
   Size:        237     429
 
PfoI T'CCnGG_A

Cuts at:      0     579     666 
   Size:        579      87
 
PstI C_TGCA'G

Cuts at:      0     314     666 
   Size:        314     352
 
SapI GCTCTTCn'nnn_

Cuts at:      0     310     666 
   Size:        310     356
 
SfcI C'TryA_G

Cuts at:      0     310     666 
   Size:        310     356
 
StyI C'CwwG_G

Cuts at:      0     191     527     666 
   Size:        191     336     139

  Fragments arranged by size: 

                 336     191     139
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     555     666 
   Size:        555     111
 

 Enzymes that do cut:

     AleI     ApoI     BclI    BfrBI     BmrI     BpmI    BseRI    BseYI
  BsiHKAI Bsp1286I    BstXI    BtgZI     EaeI     EarI  Eco57MI EcoO109I
    EcoRI    EcoRV   HincII     MscI     MslI     NsiI     PfoI     PstI
     SapI     SfcI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI