Restriction map for
pF1KB8108
(Linear) (Six-base) MAPSORT of: KIBB8108.seq Check: 8821 from: 1 to: 1434
KIBB8108.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 1434
Size: 1315 119
AflIII A'CryG_T
Cuts at: 0 1170 1399 1434
Size: 1170 229 35
Fragments arranged by size:
1170 229 35
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 866 898 1367 1399 1434
Size: 314 32 520 32 469 32 35
Fragments arranged by size:
520 469 314 35 32 32 32
ApoI r'AATT_y
Cuts at: 0 1155 1434
Size: 1155 279
BamHI G'GATC_C
Cuts at: 0 54 1434
Size: 54 1380
BbsI GAAGACnn'nnnn_
Cuts at: 0 608 958 1434
Size: 608 350 476
Fragments arranged by size:
608 476 350
BbvCI CC'TCA_GC
Cuts at: 0 92 1434
Size: 92 1342
BciVI GTATCCnnnnn_n'
Cuts at: 0 380 1434
Size: 380 1054
BglI GCCn_nnn'nGGC
Cuts at: 0 88 1182 1434
Size: 88 1094 252
Fragments arranged by size:
1094 252 88
Bpu10I CC'TnA_GC
Cuts at: 0 92 1434
Size: 92 1342
BsaAI yAC'GTr
Cuts at: 0 546 1434
Size: 546 888
BsaBI GATnn'nnATC
Cuts at: 0 583 1328 1434
Size: 583 745 106
Fragments arranged by size:
745 583 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 173 203 327 357 1434
Size: 173 30 124 30 1077
Fragments arranged by size:
1077 173 124 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 1434
Size: 28 1406
BsmI GAATG_Cn'
Cuts at: 0 406 1434
Size: 406 1028
BspHI T'CATG_A
Cuts at: 0 1122 1434
Size: 1122 312
BsrFI r'CCGG_y
Cuts at: 0 706 1434
Size: 706 728
BstXI CCAn_nnnn'nTGG
Cuts at: 0 43 1434
Size: 43 1391
BstYI r'GATC_y
Cuts at: 0 54 1323 1434
Size: 54 1269 111
Fragments arranged by size:
1269 111 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 284 1434
Size: 284 1150
EarI CTCTTCn'nnn_
Cuts at: 0 124 310 482 1434
Size: 124 186 172 952
Fragments arranged by size:
952 186 172 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 1434
Size: 1315 119
EcoO109I rG'GnC_Cy
Cuts at: 0 139 1434
Size: 139 1295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 331 363 656 688 1434
Size: 160 32 139 32 293 32 746
Fragments arranged by size:
746 293 160 139 32 32 32
MfeI C'AATT_G
Cuts at: 0 995 1085 1434
Size: 995 90 349
Fragments arranged by size:
995 349 90
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 578 1146 1420 1434
Size: 191 387 568 274 14
Fragments arranged by size:
568 387 274 191 14
MslI CAynn'nnrTG
Cuts at: 0 41 767 1338 1434
Size: 41 726 571 96
Fragments arranged by size:
726 571 96 41
MspA1I CmG'CkG
Cuts at: 0 436 1434
Size: 436 998
NspI r_CATG'y
Cuts at: 0 1018 1174 1403 1434
Size: 1018 156 229 31
Fragments arranged by size:
1018 229 156 31
PciI A'CATG_T
Cuts at: 0 1170 1399 1434
Size: 1170 229 35
Fragments arranged by size:
1170 229 35
PfoI T'CCnGG_A
Cuts at: 0 28 1434
Size: 28 1406
PstI C_TGCA'G
Cuts at: 0 150 519 1434
Size: 150 369 915
Fragments arranged by size:
915 369 150
PvuII CAG'CTG
Cuts at: 0 436 1434
Size: 436 998
SapI GCTCTTCn'nnn_
Cuts at: 0 124 1434
Size: 124 1310
SfcI C'TryA_G
Cuts at: 0 146 515 1434
Size: 146 369 919
Fragments arranged by size:
919 369 146
SnaBI TAC'GTA
Cuts at: 0 546 1434
Size: 546 888
StuI AGG'CCT
Cuts at: 0 647 1434
Size: 647 787
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 429 1434
Size: 429 1005
TatI w'GTAC_w
Cuts at: 0 1102 1434
Size: 1102 332
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 173 205 792 824 1013 1045 1434
Size: 173 32 587 32 189 32 389
Fragments arranged by size:
587 389 189 173 32 32 32
Enzymes that do cut:
AcuI AflIII AloI ApoI BamHI BbsI BbvCI BciVI
BglI Bpu10I BsaAI BsaBI BsaXI BseRI BsmI BspHI
BsrFI BstXI BstYI BtgZI EarI Eco57MI EcoO109I Hin4I
MfeI MmeI MslI MspA1I NspI PciI PfoI PstI
PvuII SapI SfcI SnaBI StuI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BcgI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI SspI StyI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI