Restriction map for
pF1KB8111
(Linear) (Six-base) MAPSORT of: KIBB8111.seq Check: 8814 from: 1 to: 1668
KIBB8111.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1149 1668
Size: 1149 519
AleI CACnn'nnGTG
Cuts at: 0 1599 1668
Size: 1599 69
ApaI G_GGCC'C
Cuts at: 0 145 1384 1668
Size: 145 1239 284
Fragments arranged by size:
1239 284 145
ApoI r'AATT_y
Cuts at: 0 332 910 1490 1527 1668
Size: 332 578 580 37 141
Fragments arranged by size:
580 578 332 141 37
AvaI C'yCGr_G
Cuts at: 0 296 1668
Size: 296 1372
BamHI G'GATC_C
Cuts at: 0 1461 1668
Size: 1461 207
BanI G'GyrC_C
Cuts at: 0 181 1343 1668
Size: 181 1162 325
Fragments arranged by size:
1162 325 181
BanII G_rGCy'C
Cuts at: 0 145 801 1384 1668
Size: 145 656 583 284
Fragments arranged by size:
656 583 284 145
BbeI G_GCGC'C
Cuts at: 0 185 1668
Size: 185 1483
BbsI GAAGACnn'nnnn_
Cuts at: 0 1106 1157 1668
Size: 1106 51 511
Fragments arranged by size:
1106 511 51
BciVI GTATCCnnnnn_n'
Cuts at: 0 988 1668
Size: 988 680
BfrBI ATG'CAT
Cuts at: 0 809 1668
Size: 809 859
BglI GCCn_nnn'nGGC
Cuts at: 0 1148 1668
Size: 1148 520
Bme1580I G_kGCm'C
Cuts at: 0 38 145 1384 1668
Size: 38 107 1239 284
Fragments arranged by size:
1239 284 107 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1378 1668
Size: 1378 290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 358 734 1668
Size: 297 61 376 934
Fragments arranged by size:
934 376 297 61
Bpu10I CC'TnA_GC
Cuts at: 0 1138 1668
Size: 1138 530
BsaI GGTCTCn'nnnn_
Cuts at: 0 529 923 1668
Size: 529 394 745
Fragments arranged by size:
745 529 394
BsaAI yAC'GTr
Cuts at: 0 1037 1668
Size: 1037 631
BsaHI Gr'CG_yC
Cuts at: 0 29 182 1060 1655 1668
Size: 29 153 878 595 13
Fragments arranged by size:
878 595 153 29 13
BsaWI w'CCGG_w
Cuts at: 0 621 1668
Size: 621 1047
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1249 1279 1668
Size: 1249 30 389
Fragments arranged by size:
1249 389 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1164 1167 1668
Size: 1164 3 501
Fragments arranged by size:
1164 501 3
BseYI C'CCAG_C
Cuts at: 0 203 1582 1668
Size: 203 1379 86
Fragments arranged by size:
1379 203 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1626 1668
Size: 1033 593 42
Fragments arranged by size:
1033 593 42
BsmI GAATG_Cn'
Cuts at: 0 119 1668
Size: 119 1549
Bsp1286I G_dGCh'C
Cuts at: 0 38 145 801 1384 1668
Size: 38 107 656 583 284
Fragments arranged by size:
656 583 284 107 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1055 1091 1149 1668
Size: 1055 36 58 519
Fragments arranged by size:
1055 519 58 36
BsrBI CCG'CTC
Cuts at: 0 390 1668
Size: 390 1278
BsrFI r'CCGG_y
Cuts at: 0 184 1086 1668
Size: 184 902 582
Fragments arranged by size:
902 582 184
BssSI C'ACGA_G
Cuts at: 0 934 1668
Size: 934 734
BstYI r'GATC_y
Cuts at: 0 1461 1613 1668
Size: 1461 152 55
Fragments arranged by size:
1461 152 55
Bsu36I CC'TnA_GG
Cuts at: 0 236 920 1668
Size: 236 684 748
Fragments arranged by size:
748 684 236
BtgI C'CryG_G
Cuts at: 0 149 281 1668
Size: 149 132 1387
Fragments arranged by size:
1387 149 132
BtsI GCAGTG_nn'
Cuts at: 0 158 1668
Size: 158 1510
DraI TTT'AAA
Cuts at: 0 1533 1668
Size: 1533 135
EaeI y'GGCC_r
Cuts at: 0 2 1668
Size: 2 1666
EarI CTCTTCn'nnn_
Cuts at: 0 1297 1668
Size: 1297 371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 200 762 1346 1668
Size: 200 562 584 322
Fragments arranged by size:
584 562 322 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 358 734 1668
Size: 297 61 376 934
Fragments arranged by size:
934 376 297 61
EcoO109I rG'GnC_Cy
Cuts at: 0 89 141 142 369 612 1668
Size: 89 52 1 227 243 1056
Fragments arranged by size:
1056 243 227 89 52 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 453 1668
Size: 453 1215
HaeII r_GCGC'y
Cuts at: 0 185 1668
Size: 185 1483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 553 585 1243 1249 1275 1281
Size: 385 32 136 32 658 6 26 6
Cuts at: 1281 1554 1586 1668
Size: 273 32 82
Fragments arranged by size:
658 385 273 136 82 32 32 32
26 6 6
HincII GTy'rAC
Cuts at: 0 1470 1668
Size: 1470 198
HindIII A'AGCT_T
Cuts at: 0 430 1668
Size: 430 1238
KasI G'GCGC_C
Cuts at: 0 181 1668
Size: 181 1487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1076 1223 1295 1668
Size: 869 207 147 72 373
Fragments arranged by size:
869 373 207 147 72
MscI TGG'CCA
Cuts at: 0 4 1668
Size: 4 1664
MslI CAynn'nnrTG
Cuts at: 0 1159 1599 1668
Size: 1159 440 69
Fragments arranged by size:
1159 440 69
MspA1I CmG'CkG
Cuts at: 0 21 1425 1586 1660 1668
Size: 21 1404 161 74 8
Fragments arranged by size:
1404 161 74 21 8
NaeI GCC'GGC
Cuts at: 0 186 1088 1668
Size: 186 902 580
Fragments arranged by size:
902 580 186
NarI GG'CG_CC
Cuts at: 0 182 1668
Size: 182 1486
NcoI C'CATG_G
Cuts at: 0 149 1668
Size: 149 1519
NgoMIV G'CCGG_C
Cuts at: 0 184 1086 1668
Size: 184 902 582
Fragments arranged by size:
902 582 184
NsiI A_TGCA'T
Cuts at: 0 811 1668
Size: 811 857
NspI r_CATG'y
Cuts at: 0 202 1158 1668
Size: 202 956 510
Fragments arranged by size:
956 510 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 825 1668
Size: 825 843
PfoI T'CCnGG_A
Cuts at: 0 1616 1668
Size: 1616 52
PmlI CAC'GTG
Cuts at: 0 1037 1668
Size: 1037 631
PpuMI rG'GwC_Cy
Cuts at: 0 89 369 612 1668
Size: 89 280 243 1056
Fragments arranged by size:
1056 280 243 89
PspOMI G'GGCC_C
Cuts at: 0 141 1380 1668
Size: 141 1239 288
Fragments arranged by size:
1239 288 141
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1265 1297 1668
Size: 1265 32 371
Fragments arranged by size:
1265 371 32
PstI C_TGCA'G
Cuts at: 0 165 197 1668
Size: 165 32 1471
Fragments arranged by size:
1471 165 32
PvuII CAG'CTG
Cuts at: 0 21 1425 1668
Size: 21 1404 243
Fragments arranged by size:
1404 243 21
ScaI AGT'ACT
Cuts at: 0 583 1668
Size: 583 1085
SfcI C'TryA_G
Cuts at: 0 161 193 1668
Size: 161 32 1475
Fragments arranged by size:
1475 161 32
SfoI GGC'GCC
Cuts at: 0 183 1668
Size: 183 1485
SmlI C'TyrA_G
Cuts at: 0 296 1668
Size: 296 1372
SphI G_CATG'C
Cuts at: 0 202 1158 1668
Size: 202 956 510
Fragments arranged by size:
956 510 202
StyI C'CwwG_G
Cuts at: 0 149 261 1339 1495 1668
Size: 149 112 1078 156 173
Fragments arranged by size:
1078 173 156 149 112
TatI w'GTAC_w
Cuts at: 0 581 1241 1668
Size: 581 660 427
Fragments arranged by size:
660 581 427
Tth111I GACn'n_nGTC
Cuts at: 0 99 1668
Size: 99 1569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 288 723 1668
Size: 288 435 945
Fragments arranged by size:
945 435 288
XhoI C'TCGA_G
Cuts at: 0 296 1668
Size: 296 1372
Enzymes that do cut:
AarI AleI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BciVI BfrBI BglI Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PflMI PfoI PmlI PpuMI PspOMI PsrI PstI
PvuII ScaI SfcI SfoI SmlI SphI StyI TatI
Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BclI BglII BlpI
BmgBI BmtI BplI BpuEI BsaBI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
PacI PasI PciI PmeI PpiI PshAI PsiI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TstI XbaI XmaI XmnI
ZraI