(Linear) (Six-base) MAPSORT of: KIBB8112.seq  Check: 9560  from: 1  to: 456



KIBB8112.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     239     456 
   Size:        239     217
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     328     456 
   Size:        328     128
 
AlwNI CAG_nnn'CTG

Cuts at:      0      61     456 
   Size:         61     395
 
ApoI r'AATT_y

Cuts at:      0     221     456 
   Size:        221     235
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     394     456 
   Size:        394      62
 
BseYI C'CCAG_C

Cuts at:      0      35     288     456 
   Size:         35     253     168

  Fragments arranged by size: 

                 253     168      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     342     456 
   Size:        342     114
 
Bsp1286I G_dGCh'C

Cuts at:      0     342     456 
   Size:        342     114
 
BspHI T'CATG_A

Cuts at:      0     212     456 
   Size:        212     244
 
BsrDI GCAATG_nn'

Cuts at:      0     113     456 
   Size:        113     343
 
BstYI r'GATC_y

Cuts at:      0     299     456 
   Size:        299     157
 
Bsu36I CC'TnA_GG

Cuts at:      0     125     456 
   Size:        125     331
 
BtsI GCAGTG_nn'

Cuts at:      0     292     388     456 
   Size:        292      96      68

  Fragments arranged by size: 

                 292      96      68
 
ClaI AT'CG_AT

Cuts at:      0     319     456 
   Size:        319     137
 
DraI TTT'AAA

Cuts at:      0     156     456 
   Size:        156     300
 
EaeI y'GGCC_r

Cuts at:      0       2     456 
   Size:          2     454
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     456 
   Size:        268      32     156

  Fragments arranged by size: 

                 268     156      32
 
MslI CAynn'nnrTG

Cuts at:      0     233     456 
   Size:        233     223
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      61     456 
   Size:         61     395
 
PstI C_TGCA'G

Cuts at:      0     299     456 
   Size:        299     157
 
SfcI C'TryA_G

Cuts at:      0     295     456 
   Size:        295     161
 
SspI AAT'ATT

Cuts at:      0     453     456 
   Size:        453       3
 
StuI AGG'CCT

Cuts at:      0     328     456 
   Size:        328     128
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     284     316     456 
   Size:        284      32     140

  Fragments arranged by size: 

                 284     140      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      56     456 
   Size:         56     400
 

 Enzymes that do cut:

     AccI     AlfI    AlwNI     ApoI     BbsI    BseYI  BsiHKAI Bsp1286I
    BspHI    BsrDI    BstYI   Bsu36I     BtsI     ClaI     DraI     EaeI
    Hin4I     MslI    PflMI     PstI     SfcI     SspI     StuI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI    CspCI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI