Restriction map for
pF1KB8113
(Linear) (Six-base) MAPSORT of: KIBB8113.seq Check: 3868 from: 1 to: 1140
KIBB8113.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 279 787 1140
Size: 279 508 353
Fragments arranged by size:
508 353 279
AccI GT'mk_AC
Cuts at: 0 95 1140
Size: 95 1045
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 1140
Size: 567 573
AflIII A'CryG_T
Cuts at: 0 90 1140
Size: 90 1050
AleI CACnn'nnGTG
Cuts at: 0 865 1140
Size: 865 275
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 614 646 1140
Size: 614 32 494
Fragments arranged by size:
614 494 32
AlwNI CAG_nnn'CTG
Cuts at: 0 942 1140
Size: 942 198
BamHI G'GATC_C
Cuts at: 0 848 1140
Size: 848 292
BanI G'GyrC_C
Cuts at: 0 231 1140
Size: 231 909
BanII G_rGCy'C
Cuts at: 0 326 884 1045 1140
Size: 326 558 161 95
Fragments arranged by size:
558 326 161 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 792 1140
Size: 83 709 348
Fragments arranged by size:
709 348 83
BglI GCCn_nnn'nGGC
Cuts at: 0 451 1140
Size: 451 689
BglII A'GATC_T
Cuts at: 0 111 1140
Size: 111 1029
Bme1580I G_kGCm'C
Cuts at: 0 234 1140
Size: 234 906
BmgBI CAC'GTC
Cuts at: 0 91 1140
Size: 91 1049
BmrI ACTGGGnnnn_n'
Cuts at: 0 374 528 1140
Size: 374 154 612
Fragments arranged by size:
612 374 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 757 898 1140
Size: 432 325 141 242
Fragments arranged by size:
432 325 242 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1140
Size: 373 767
BsaAI yAC'GTr
Cuts at: 0 177 1140
Size: 177 963
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 376 1069 1088 1140
Size: 376 693 19 52
Fragments arranged by size:
693 376 52 19
BseYI C'CCAG_C
Cuts at: 0 691 1066 1140
Size: 691 375 74
Fragments arranged by size:
691 375 74
BsiHKAI G_wGCw'C
Cuts at: 0 523 884 1045 1140
Size: 523 361 161 95
Fragments arranged by size:
523 361 161 95
Bsp1286I G_dGCh'C
Cuts at: 0 234 326 523 884 1045 1140
Size: 234 92 197 361 161 95
Fragments arranged by size:
361 234 197 161 95 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 279 740 787 1140
Size: 279 461 47 353
Fragments arranged by size:
461 353 279 47
BsrBI CCG'CTC
Cuts at: 0 320 1140
Size: 320 820
BsrDI GCAATG_nn'
Cuts at: 0 440 587 855 1140
Size: 440 147 268 285
Fragments arranged by size:
440 285 268 147
BsrFI r'CCGG_y
Cuts at: 0 374 445 1085 1140
Size: 374 71 640 55
Fragments arranged by size:
640 374 71 55
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 1140
Size: 291 849
BstYI r'GATC_y
Cuts at: 0 111 848 1140
Size: 111 737 292
Fragments arranged by size:
737 292 111
BtsI GCAGTG_nn'
Cuts at: 0 1026 1140
Size: 1026 114
DraIII CAC_nnn'GTG
Cuts at: 0 639 1140
Size: 639 501
EaeI y'GGCC_r
Cuts at: 0 330 452 641 1101 1140
Size: 330 122 189 460 39
Fragments arranged by size:
460 330 189 122 39
EarI CTCTTCn'nnn_
Cuts at: 0 148 586 726 1140
Size: 148 438 140 414
Fragments arranged by size:
438 414 148 140
EcoICRI GAG'CTC
Cuts at: 0 882 1043 1140
Size: 882 161 97
Fragments arranged by size:
882 161 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 567 757 898 1140
Size: 432 135 190 141 242
Fragments arranged by size:
432 242 190 141 135
EcoRV GAT'ATC
Cuts at: 0 508 1140
Size: 508 632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 319 1140
Size: 319 821
FspI TGC'GCA
Cuts at: 0 973 1140
Size: 973 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 818 850 1140
Size: 292 32 494 32 290
Fragments arranged by size:
494 292 290 32 32
MscI TGG'CCA
Cuts at: 0 643 1103 1140
Size: 643 460 37
Fragments arranged by size:
643 460 37
MslI CAynn'nnrTG
Cuts at: 0 865 1140
Size: 865 275
MspA1I CmG'CkG
Cuts at: 0 410 939 1066 1140
Size: 410 529 127 74
Fragments arranged by size:
529 410 127 74
NaeI GCC'GGC
Cuts at: 0 447 1087 1140
Size: 447 640 53
Fragments arranged by size:
640 447 53
NgoMIV G'CCGG_C
Cuts at: 0 445 1085 1140
Size: 445 640 55
Fragments arranged by size:
640 445 55
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 1140
Size: 639 501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 1140
Size: 334 32 774
Fragments arranged by size:
774 334 32
PstI C_TGCA'G
Cuts at: 0 1065 1140
Size: 1065 75
PvuII CAG'CTG
Cuts at: 0 410 939 1066 1140
Size: 410 529 127 74
Fragments arranged by size:
529 410 127 74
SacI G_AGCT'C
Cuts at: 0 884 1045 1140
Size: 884 161 95
Fragments arranged by size:
884 161 95
SapI GCTCTTCn'nnn_
Cuts at: 0 148 586 1140
Size: 148 438 554
Fragments arranged by size:
554 438 148
SfcI C'TryA_G
Cuts at: 0 934 1061 1140
Size: 934 127 79
Fragments arranged by size:
934 127 79
SmlI C'TyrA_G
Cuts at: 0 388 1140
Size: 388 752
StyI C'CwwG_G
Cuts at: 0 248 1140
Size: 248 892
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 95 1140
Size: 95 1045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 25 57 1140
Size: 25 32 1083
Fragments arranged by size:
1083 32 25
XbaI T'CTAG_A
Cuts at: 0 72 1140
Size: 72 1068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 1140
Size: 246 894
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AloI AlwNI BamHI
BanI BanII BbsI BglI BglII Bme1580I BmgBI BmrI
BpmI BpuEI BsaAI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstXI BstYI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoRV FalI FspI Hin4I MscI
MslI MspA1I NaeI NgoMIV PflMI PpiI PstI PvuII
SacI SapI SfcI SmlI StyI TaqII TstI XbaI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoO109I EcoRI FseI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XhoI
XmaI XmnI ZraI