Restriction map for
pF1KB8115
(Linear) (Six-base) MAPSORT of: KIBB8115.seq Check: 4318 from: 1 to: 1200
KIBB8115.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 172 502 1200
Size: 124 48 330 698
Fragments arranged by size:
698 330 124 48
ApoI r'AATT_y
Cuts at: 0 12 322 402 652 918 979 1025 1200
Size: 12 310 80 250 266 61 46 175
Fragments arranged by size:
310 266 250 175 80 61 46 12
AvrII C'CTAG_G
Cuts at: 0 579 1200
Size: 579 621
BanII G_rGCy'C
Cuts at: 0 178 812 1200
Size: 178 634 388
Fragments arranged by size:
634 388 178
BbsI GAAGACnn'nnnn_
Cuts at: 0 130 1073 1200
Size: 130 943 127
Fragments arranged by size:
943 130 127
BglII A'GATC_T
Cuts at: 0 744 1200
Size: 744 456
BlpI GC'TnA_GC
Cuts at: 0 596 1200
Size: 596 604
BmtI G_CTAG'C
Cuts at: 0 886 1200
Size: 886 314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 678 1200
Size: 382 296 522
Fragments arranged by size:
522 382 296
BsaWI w'CCGG_w
Cuts at: 0 109 1200
Size: 109 1091
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 731 761 1200
Size: 731 30 439
Fragments arranged by size:
731 439 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 68 820 1200
Size: 68 752 380
Fragments arranged by size:
752 380 68
BseYI C'CCAG_C
Cuts at: 0 993 1200
Size: 993 207
BsiHKAI G_wGCw'C
Cuts at: 0 812 1200
Size: 812 388
Bsp1286I G_dGCh'C
Cuts at: 0 178 812 1200
Size: 178 634 388
Fragments arranged by size:
634 388 178
BspEI T'CCGG_A
Cuts at: 0 109 1200
Size: 109 1091
BspHI T'CATG_A
Cuts at: 0 267 1200
Size: 267 933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1051 1200
Size: 1051 149
BsrDI GCAATG_nn'
Cuts at: 0 224 832 1200
Size: 224 608 368
Fragments arranged by size:
608 368 224
BsrGI T'GTAC_A
Cuts at: 0 456 858 1200
Size: 456 402 342
Fragments arranged by size:
456 402 342
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1175 1200
Size: 1175 25
BstYI r'GATC_y
Cuts at: 0 744 1032 1200
Size: 744 288 168
Fragments arranged by size:
744 288 168
BtgI C'CryG_G
Cuts at: 0 148 1200
Size: 148 1052
BtsI GCAGTG_nn'
Cuts at: 0 1173 1200
Size: 1173 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1080 1115 1200
Size: 1080 35 85
Fragments arranged by size:
1080 85 35
EarI CTCTTCn'nnn_
Cuts at: 0 828 1200
Size: 828 372
EcoICRI GAG'CTC
Cuts at: 0 810 1200
Size: 810 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 172 502 1200
Size: 124 48 330 698
Fragments arranged by size:
698 330 124 48
EcoRI G'AATT_C
Cuts at: 0 322 402 979 1200
Size: 322 80 577 221
Fragments arranged by size:
577 322 221 80
EcoRV GAT'ATC
Cuts at: 0 273 1200
Size: 273 927
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 454 719 1200
Size: 454 265 481
Fragments arranged by size:
481 454 265
HincII GTy'rAC
Cuts at: 0 686 1200
Size: 686 514
HpaI GTT'AAC
Cuts at: 0 686 1200
Size: 686 514
MfeI C'AATT_G
Cuts at: 0 1161 1200
Size: 1161 39
MspA1I CmG'CkG
Cuts at: 0 997 1200
Size: 997 203
NcoI C'CATG_G
Cuts at: 0 148 1200
Size: 148 1052
NheI G'CTAG_C
Cuts at: 0 882 1200
Size: 882 318
PstI C_TGCA'G
Cuts at: 0 988 1200
Size: 988 212
PvuII CAG'CTG
Cuts at: 0 997 1200
Size: 997 203
SacI G_AGCT'C
Cuts at: 0 812 1200
Size: 812 388
SfcI C'TryA_G
Cuts at: 0 421 984 1200
Size: 421 563 216
Fragments arranged by size:
563 421 216
SmlI C'TyrA_G
Cuts at: 0 397 693 1200
Size: 397 296 507
Fragments arranged by size:
507 397 296
SspI AAT'ATT
Cuts at: 0 242 1116 1200
Size: 242 874 84
Fragments arranged by size:
874 242 84
StyI C'CwwG_G
Cuts at: 0 148 327 579 1200
Size: 148 179 252 621
Fragments arranged by size:
621 252 179 148
TatI w'GTAC_w
Cuts at: 0 456 858 1200
Size: 456 402 342
Fragments arranged by size:
456 402 342
XbaI T'CTAG_A
Cuts at: 0 121 1200
Size: 121 1079
XmnI GAAnn'nnTTC
Cuts at: 0 767 1016 1200
Size: 767 249 184
Fragments arranged by size:
767 249 184
Enzymes that do cut:
AcuI ApoI AvrII BanII BbsI BglII BlpI BmtI
BpuEI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrDI BsrGI BstAPI BstYI BtgI BtsI
CspCI EarI EcoICRI Eco57MI EcoRI EcoRV FalI HincII
HpaI MfeI MspA1I NcoI NheI PstI PvuII SacI
SfcI SmlI SspI StyI TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsgI BsiEI BsiWI BsmI BsmBI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EaeI EagI EciI EcoNI EcoO109I
FseI FspI FspAI HaeII Hin4I HindIII KasI KpnI
MluI MmeI MscI MslI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XcmI XhoI XmaI ZraI