Restriction map for
pF1KB8124
(Linear) (Six-base) MAPSORT of: KIBB8124.seq Check: 8741 from: 1 to: 1197
KIBB8124.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 569 1197
Size: 569 628
AccI GT'mk_AC
Cuts at: 0 127 1197
Size: 127 1070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 722 1197
Size: 559 163 475
Fragments arranged by size:
559 475 163
AgeI A'CCGG_T
Cuts at: 0 475 1197
Size: 475 722
AlwNI CAG_nnn'CTG
Cuts at: 0 537 1197
Size: 537 660
ApaI G_GGCC'C
Cuts at: 0 41 307 351 1197
Size: 41 266 44 846
Fragments arranged by size:
846 266 44 41
ApoI r'AATT_y
Cuts at: 0 1150 1183 1197
Size: 1150 33 14
Fragments arranged by size:
1150 33 14
AvaI C'yCGr_G
Cuts at: 0 175 199 222 343 773 1197
Size: 175 24 23 121 430 424
Fragments arranged by size:
430 424 175 121 24 23
BanI G'GyrC_C
Cuts at: 0 151 361 1197
Size: 151 210 836
Fragments arranged by size:
836 210 151
BanII G_rGCy'C
Cuts at: 0 41 176 307 351 511 610 808 1197
Size: 41 135 131 44 160 99 198 389
Fragments arranged by size:
389 198 160 135 131 99 44 41
BbeI G_GCGC'C
Cuts at: 0 365 1197
Size: 365 832
BbsI GAAGACnn'nnnn_
Cuts at: 0 872 1197
Size: 872 325
BciVI GTATCCnnnnn_n'
Cuts at: 0 1119 1197
Size: 1119 78
BglI GCCn_nnn'nGGC
Cuts at: 0 316 384 1197
Size: 316 68 813
Fragments arranged by size:
813 316 68
Bme1580I G_kGCm'C
Cuts at: 0 41 307 351 1197
Size: 41 266 44 846
Fragments arranged by size:
846 266 44 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 525 885 1197
Size: 525 360 312
Fragments arranged by size:
525 360 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 307 674 1048 1197
Size: 301 6 367 374 149
Fragments arranged by size:
374 367 301 149 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 610 1197
Size: 538 72 587
Fragments arranged by size:
587 538 72
BsaAI yAC'GTr
Cuts at: 0 96 441 1197
Size: 96 345 756
Fragments arranged by size:
756 345 96
BsaBI GATnn'nnATC
Cuts at: 0 845 1073 1197
Size: 845 228 124
Fragments arranged by size:
845 228 124
BsaHI Gr'CG_yC
Cuts at: 0 362 1197
Size: 362 835
BsaWI w'CCGG_w
Cuts at: 0 475 1197
Size: 475 722
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 94 565 595 1197
Size: 64 30 471 30 602
Fragments arranged by size:
602 471 64 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 603 655 799 904 1197
Size: 603 52 144 105 293
Fragments arranged by size:
603 293 144 105 52
BseYI C'CCAG_C
Cuts at: 0 103 194 300 463 588 1197
Size: 103 91 106 163 125 609
Fragments arranged by size:
609 163 125 106 103 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 187 1197
Size: 124 63 1010
Fragments arranged by size:
1010 124 63
BsiHKAI G_wGCw'C
Cuts at: 0 511 1161 1197
Size: 511 650 36
Fragments arranged by size:
650 511 36
Bsp1286I G_dGCh'C
Cuts at: 0 41 176 307 351 511 610 808 1161
Size: 41 135 131 44 160 99 198 353
Cuts at: 1161 1197
Size: 36
Fragments arranged by size:
353 198 160 135 131 99 44 41
36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 569 593 1197
Size: 569 24 604
Fragments arranged by size:
604 569 24
BsrBI CCG'CTC
Cuts at: 0 458 1197
Size: 458 739
BsrFI r'CCGG_y
Cuts at: 0 325 475 600 1197
Size: 325 150 125 597
Fragments arranged by size:
597 325 150 125
BssSI C'ACGA_G
Cuts at: 0 525 1197
Size: 525 672
BstAPI GCAn_nnn'nTGC
Cuts at: 0 158 1197
Size: 158 1039
BstBI TT'CG_AA
Cuts at: 0 1188 1197
Size: 1188 9
BstYI r'GATC_y
Cuts at: 0 981 1197
Size: 981 216
Bsu36I CC'TnA_GG
Cuts at: 0 860 1197
Size: 860 337
BtgI C'CryG_G
Cuts at: 0 365 656 1197
Size: 365 291 541
Fragments arranged by size:
541 365 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 25 511 1197
Size: 25 486 686
Fragments arranged by size:
686 486 25
DraIII CAC_nnn'GTG
Cuts at: 0 158 1197
Size: 158 1039
EaeI y'GGCC_r
Cuts at: 0 110 212 353 1197
Size: 110 102 141 844
Fragments arranged by size:
844 141 110 102
EarI CTCTTCn'nnn_
Cuts at: 0 499 701 1197
Size: 499 202 496
Fragments arranged by size:
499 496 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 334 411 652 1029 1197
Size: 19 315 77 241 377 168
Fragments arranged by size:
377 315 241 168 77 19
EcoICRI GAG'CTC
Cuts at: 0 509 1197
Size: 509 688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 307 559 674 722 1048 1197
Size: 301 6 252 115 48 326 149
Fragments arranged by size:
326 301 252 149 115 48 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 1197
Size: 355 842
EcoO109I rG'GnC_Cy
Cuts at: 0 304 347 348 645 1197
Size: 304 43 1 297 552
Fragments arranged by size:
552 304 297 43 1
EcoRV GAT'ATC
Cuts at: 0 1075 1197
Size: 1075 122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 993 1197
Size: 993 204
HaeII r_GCGC'y
Cuts at: 0 211 365 1197
Size: 211 154 832
Fragments arranged by size:
832 211 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 220 252 313 345 1197
Size: 64 32 124 32 61 32 852
Fragments arranged by size:
852 124 64 61 32 32 32
HincII GTy'rAC
Cuts at: 0 128 1197
Size: 128 1069
KasI G'GCGC_C
Cuts at: 0 361 1197
Size: 361 836
MspA1I CmG'CkG
Cuts at: 0 367 384 658 1197
Size: 367 17 274 539
Fragments arranged by size:
539 367 274 17
NarI GG'CG_CC
Cuts at: 0 362 1197
Size: 362 835
PfoI T'CCnGG_A
Cuts at: 0 251 728 1197
Size: 251 477 469
Fragments arranged by size:
477 469 251
PpuMI rG'GwC_Cy
Cuts at: 0 645 1197
Size: 645 552
PspOMI G'GGCC_C
Cuts at: 0 37 303 347 1197
Size: 37 266 44 850
Fragments arranged by size:
850 266 44 37
SacI G_AGCT'C
Cuts at: 0 511 1197
Size: 511 686
SacII CC_GC'GG
Cuts at: 0 368 659 1197
Size: 368 291 538
Fragments arranged by size:
538 368 291
SalI G'TCGA_C
Cuts at: 0 126 1197
Size: 126 1071
SapI GCTCTTCn'nnn_
Cuts at: 0 499 1197
Size: 499 698
SfoI GGC'GCC
Cuts at: 0 363 1197
Size: 363 834
SgrAI Cr'CCGG_yG
Cuts at: 0 600 1197
Size: 600 597
SmaI CCC'GGG
Cuts at: 0 177 201 345 775 1197
Size: 177 24 144 430 422
Fragments arranged by size:
430 422 177 144 24
SmlI C'TyrA_G
Cuts at: 0 553 625 1197
Size: 553 72 572
Fragments arranged by size:
572 553 72
StuI AGG'CCT
Cuts at: 0 220 292 1003 1197
Size: 220 72 711 194
Fragments arranged by size:
711 220 194 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 867 1197
Size: 58 809 330
Fragments arranged by size:
809 330 58
TatI w'GTAC_w
Cuts at: 0 245 1197
Size: 245 952
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 989 1021 1197
Size: 989 32 176
Fragments arranged by size:
989 176 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1137 1197
Size: 1137 60
XmaI C'CCGG_G
Cuts at: 0 175 199 343 773 1197
Size: 175 24 144 430 424
Fragments arranged by size:
430 424 175 144 24
Enzymes that do cut:
AarI AccI AcuI AgeI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BciVI BglI Bme1580I BplI
BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII KasI MspA1I NarI PfoI PpuMI
PspOMI SacI SacII SalI SapI SfoI SgrAI SmaI
SmlI StuI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI Bpu10I BsaI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BstEII BstXI BstZ17I
BtsI ClaI CspCI DraI DrdI EagI EcoRI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SanDI SbfI ScaI SexAI SfcI SfiI
SgfI SnaBI SpeI SphI SrfI SspI StyI SwaI
Tth111I XbaI XhoI XmnI ZraI