Restriction map for
pF1KB8126
(Linear) (Six-base) MAPSORT of: KIBB8126.seq Check: 632 from: 1 to: 1659
KIBB8126.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
Acc65I G'GTAC_C
Cuts at: 0 1291 1659
Size: 1291 368
AclI AA'CG_TT
Cuts at: 0 690 1226 1659
Size: 690 536 433
Fragments arranged by size:
690 536 433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 838 1432 1659
Size: 281 557 594 227
Fragments arranged by size:
594 557 281 227
AflII C'TTAA_G
Cuts at: 0 222 1659
Size: 222 1437
AflIII A'CryG_T
Cuts at: 0 502 1659
Size: 502 1157
AleI CACnn'nnGTG
Cuts at: 0 817 1659
Size: 817 842
AlwNI CAG_nnn'CTG
Cuts at: 0 861 1659
Size: 861 798
ApoI r'AATT_y
Cuts at: 0 545 1635 1659
Size: 545 1090 24
Fragments arranged by size:
1090 545 24
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 179 212 841 874 1191 1224 1659
Size: 179 33 629 33 317 33 435
Fragments arranged by size:
629 435 317 179 33 33 33
BamHI G'GATC_C
Cuts at: 0 1252 1659
Size: 1252 407
BanI G'GyrC_C
Cuts at: 0 597 1291 1659
Size: 597 694 368
Fragments arranged by size:
694 597 368
BanII G_rGCy'C
Cuts at: 0 1502 1659
Size: 1502 157
BbvCI CC'TCA_GC
Cuts at: 0 259 1659
Size: 259 1400
BciVI GTATCCnnnnn_n'
Cuts at: 0 1219 1659
Size: 1219 440
BclI T'GATC_A
Cuts at: 0 923 1659
Size: 923 736
BglII A'GATC_T
Cuts at: 0 1175 1592 1659
Size: 1175 417 67
Fragments arranged by size:
1175 417 67
Bme1580I G_kGCm'C
Cuts at: 0 1573 1659
Size: 1573 86
BmrI ACTGGGnnnn_n'
Cuts at: 0 146 221 656 765 1335 1659
Size: 146 75 435 109 570 324
Fragments arranged by size:
570 435 324 146 109 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 1659
Size: 171 1488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 1066 1516 1659
Size: 48 1018 450 143
Fragments arranged by size:
1018 450 143 48
Bpu10I CC'TnA_GC
Cuts at: 0 259 1659
Size: 259 1400
BsaI GGTCTCn'nnnn_
Cuts at: 0 66 1659
Size: 66 1593
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 374 395 404 425 1018 1048 1659
Size: 374 21 9 21 593 30 611
Fragments arranged by size:
611 593 374 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 1659
Size: 445 1214
BseYI C'CCAG_C
Cuts at: 0 1501 1659
Size: 1501 158
BsiEI CG_ry'CG
Cuts at: 0 26 1659
Size: 26 1633
BsmI GAATG_Cn'
Cuts at: 0 1530 1659
Size: 1530 129
Bsp1286I G_dGCh'C
Cuts at: 0 1502 1573 1659
Size: 1502 71 86
Fragments arranged by size:
1502 86 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1272 1279 1659
Size: 1272 7 380
Fragments arranged by size:
1272 380 7
BsrDI GCAATG_nn'
Cuts at: 0 89 1659
Size: 89 1570
BssSI C'ACGA_G
Cuts at: 0 1244 1659
Size: 1244 415
BstEII G'GTnAC_C
Cuts at: 0 634 1659
Size: 634 1025
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1302 1659
Size: 1302 357
BstYI r'GATC_y
Cuts at: 0 703 757 1175 1252 1592 1659
Size: 703 54 418 77 340 67
Fragments arranged by size:
703 418 340 77 67 54
BtgI C'CryG_G
Cuts at: 0 26 884 1433 1659
Size: 26 858 549 226
Fragments arranged by size:
858 549 226 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 995 1659
Size: 995 664
EaeI y'GGCC_r
Cuts at: 0 23 1659
Size: 23 1636
EagI C'GGCC_G
Cuts at: 0 23 1659
Size: 23 1636
EarI CTCTTCn'nnn_
Cuts at: 0 161 388 775 1659
Size: 161 227 387 884
Fragments arranged by size:
884 387 227 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 973 1201 1659
Size: 973 228 458
Fragments arranged by size:
973 458 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 281 838 1066 1432 1516 1659
Size: 48 233 557 228 366 84 143
Fragments arranged by size:
557 366 233 228 143 84 48
EcoRV GAT'ATC
Cuts at: 0 1483 1659
Size: 1483 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 925 957 1659
Size: 925 32 702
Fragments arranged by size:
925 702 32
KpnI G_GTAC'C
Cuts at: 0 1295 1659
Size: 1295 364
MfeI C'AATT_G
Cuts at: 0 488 602 659 1659
Size: 488 114 57 1000
Fragments arranged by size:
1000 488 114 57
MluI A'CGCG_T
Cuts at: 0 502 1659
Size: 502 1157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1230 1659
Size: 1168 62 429
Fragments arranged by size:
1168 429 62
MslI CAynn'nnrTG
Cuts at: 0 817 820 1659
Size: 817 3 839
Fragments arranged by size:
839 817 3
MspA1I CmG'CkG
Cuts at: 0 1258 1659
Size: 1258 401
NcoI C'CATG_G
Cuts at: 0 884 1433 1659
Size: 884 549 226
Fragments arranged by size:
884 549 226
NspI r_CATG'y
Cuts at: 0 919 1659
Size: 919 740
SapI GCTCTTCn'nnn_
Cuts at: 0 161 1659
Size: 161 1498
SmlI C'TyrA_G
Cuts at: 0 222 1659
Size: 222 1437
StuI AGG'CCT
Cuts at: 0 1006 1659
Size: 1006 653
StyI C'CwwG_G
Cuts at: 0 459 884 1433 1659
Size: 459 425 549 226
Fragments arranged by size:
549 459 425 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 653 1659
Size: 653 1006
TatI w'GTAC_w
Cuts at: 0 725 809 1659
Size: 725 84 850
Fragments arranged by size:
850 725 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 374 395 406 427 1659
Size: 374 21 11 21 1232
Fragments arranged by size:
1232 374 21 21 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 72 1659
Size: 72 1587
XmnI GAAnn'nnTTC
Cuts at: 0 691 1528 1659
Size: 691 837 131
Fragments arranged by size:
837 691 131
Enzymes that do cut:
Acc65I AclI AcuI AflII AflIII AleI AlwNI ApoI
BaeI BamHI BanI BanII BbvCI BciVI BclI BglII
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaXI BseRI
BseYI BsiEI BsmI Bsp1286I BspMI BsrDI BssSI BstEII
BstXI BstYI BtgI DrdI EaeI EagI EarI EciI
Eco57MI EcoRV Hin4I KpnI MfeI MluI MmeI MslI
MspA1I NcoI NspI SapI SmlI StuI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AgeI AhdI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BbeI
BbsI BcgI BfrBI BglI BlpI BmgBI BmtI BpuEI
BsaAI BsaBI BsaHI BsaWI BsgI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI