Restriction map for
pF1KB8135
(Linear) (Six-base) MAPSORT of: KIBB8135.seq Check: 9704 from: 1 to: 1491
KIBB8135.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AatII G_ACGT'C
Cuts at: 0 905 1491
Size: 905 586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1225 1491
Size: 184 1041 266
Fragments arranged by size:
1041 266 184
AflII C'TTAA_G
Cuts at: 0 663 1491
Size: 663 828
AflIII A'CryG_T
Cuts at: 0 174 1491
Size: 174 1317
AhdI GACnn_n'nnGTC
Cuts at: 0 849 1491
Size: 849 642
AleI CACnn'nnGTG
Cuts at: 0 145 1491
Size: 145 1346
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 145 177 1491
Size: 145 32 1314
Fragments arranged by size:
1314 145 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1021 1203 1429 1491
Size: 1021 182 226 62
Fragments arranged by size:
1021 226 182 62
ApaLI G'TGCA_C
Cuts at: 0 1338 1491
Size: 1338 153
AvaI C'yCGr_G
Cuts at: 0 13 807 825 1491
Size: 13 794 18 666
Fragments arranged by size:
794 666 18 13
BanI G'GyrC_C
Cuts at: 0 31 413 1104 1491
Size: 31 382 691 387
Fragments arranged by size:
691 387 382 31
BanII G_rGCy'C
Cuts at: 0 14 1491
Size: 14 1477
BbeI G_GCGC'C
Cuts at: 0 35 1491
Size: 35 1456
BbsI GAAGACnn'nnnn_
Cuts at: 0 126 897 1213 1491
Size: 126 771 316 278
Fragments arranged by size:
771 316 278 126
BglI GCCn_nnn'nGGC
Cuts at: 0 781 1491
Size: 781 710
Bme1580I G_kGCm'C
Cuts at: 0 20 834 1342 1491
Size: 20 814 508 149
Fragments arranged by size:
814 508 149 20
BmgBI CAC'GTC
Cuts at: 0 143 1491
Size: 143 1348
BmrI ACTGGGnnnn_n'
Cuts at: 0 384 667 1491
Size: 384 283 824
Fragments arranged by size:
824 384 283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1491
Size: 1150 341
Bpu10I CC'TnA_GC
Cuts at: 0 456 965 1491
Size: 456 509 526
Fragments arranged by size:
526 509 456
BsaI GGTCTCn'nnnn_
Cuts at: 0 265 1491
Size: 265 1226
BsaAI yAC'GTr
Cuts at: 0 1458 1491
Size: 1458 33
BsaHI Gr'CG_yC
Cuts at: 0 32 902 1491
Size: 32 870 589
Fragments arranged by size:
870 589 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 481 511 1351 1381 1491
Size: 481 30 840 30 110
Fragments arranged by size:
840 481 110 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 314 1491
Size: 314 1177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1491
Size: 958 533
BsiEI CG_ry'CG
Cuts at: 0 465 1320 1491
Size: 465 855 171
Fragments arranged by size:
855 465 171
BsiHKAI G_wGCw'C
Cuts at: 0 1342 1491
Size: 1342 149
BsiWI C'GTAC_G
Cuts at: 0 408 1491
Size: 408 1083
BsmI GAATG_Cn'
Cuts at: 0 560 1491
Size: 560 931
Bsp1286I G_dGCh'C
Cuts at: 0 14 20 834 1342 1491
Size: 14 6 814 508 149
Fragments arranged by size:
814 508 149 14 6
BsrBI CCG'CTC
Cuts at: 0 1290 1491
Size: 1290 201
BsrDI GCAATG_nn'
Cuts at: 0 763 1491
Size: 763 728
BsrFI r'CCGG_y
Cuts at: 0 1342 1491
Size: 1342 149
BsrGI T'GTAC_A
Cuts at: 0 437 1491
Size: 437 1054
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1346 1491
Size: 1346 145
BstEII G'GTnAC_C
Cuts at: 0 87 1482 1491
Size: 87 1395 9
Fragments arranged by size:
1395 87 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 256 1491
Size: 147 109 1235
Fragments arranged by size:
1235 147 109
BstYI r'GATC_y
Cuts at: 0 605 1271 1491
Size: 605 666 220
Fragments arranged by size:
666 605 220
BtgI C'CryG_G
Cuts at: 0 521 779 854 1144 1491
Size: 521 258 75 290 347
Fragments arranged by size:
521 347 290 258 75
BtsI GCAGTG_nn'
Cuts at: 0 592 866 1491
Size: 592 274 625
Fragments arranged by size:
625 592 274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 495 530 1491
Size: 495 35 961
Fragments arranged by size:
961 495 35
EaeI y'GGCC_r
Cuts at: 0 104 345 462 1317 1416 1491
Size: 104 241 117 855 99 75
Fragments arranged by size:
855 241 117 104 99 75
EagI C'GGCC_G
Cuts at: 0 462 1317 1491
Size: 462 855 174
Fragments arranged by size:
855 462 174
EarI CTCTTCn'nnn_
Cuts at: 0 525 1160 1491
Size: 525 635 331
Fragments arranged by size:
635 525 331
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1491
Size: 22 1469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1150 1225 1491
Size: 184 966 75 266
Fragments arranged by size:
966 266 184 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 595 1491
Size: 595 896
EcoO109I rG'GnC_Cy
Cuts at: 0 39 313 378 1491
Size: 39 274 65 1113
Fragments arranged by size:
1113 274 65 39
HaeII r_GCGC'y
Cuts at: 0 35 1491
Size: 35 1456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 461 493 835 867 1491
Size: 461 32 342 32 624
Fragments arranged by size:
624 461 342 32 32
HincII GTy'rAC
Cuts at: 0 1055 1176 1311 1359 1491
Size: 1055 121 135 48 132
Fragments arranged by size:
1055 135 132 121 48
HpaI GTT'AAC
Cuts at: 0 1311 1491
Size: 1311 180
KasI G'GCGC_C
Cuts at: 0 31 1491
Size: 31 1460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1491
Size: 352 1139
MscI TGG'CCA
Cuts at: 0 106 1491
Size: 106 1385
MslI CAynn'nnrTG
Cuts at: 0 25 145 254 1491
Size: 25 120 109 1237
Fragments arranged by size:
1237 120 109 25
MspA1I CmG'CkG
Cuts at: 0 67 428 781 995 1146 1203 1429 1491
Size: 67 361 353 214 151 57 226 62
Fragments arranged by size:
361 353 226 214 151 67 62 57
NarI GG'CG_CC
Cuts at: 0 32 1491
Size: 32 1459
NcoI C'CATG_G
Cuts at: 0 521 1491
Size: 521 970
NdeI CA'TA_TG
Cuts at: 0 1411 1491
Size: 1411 80
NspI r_CATG'y
Cuts at: 0 178 1234 1491
Size: 178 1056 257
Fragments arranged by size:
1056 257 178
PasI CC'CwG_GG
Cuts at: 0 894 1491
Size: 894 597
PciI A'CATG_T
Cuts at: 0 174 1491
Size: 174 1317
PfoI T'CCnGG_A
Cuts at: 0 596 1491
Size: 596 895
PmlI CAC'GTG
Cuts at: 0 1458 1491
Size: 1458 33
PpuMI rG'GwC_Cy
Cuts at: 0 313 378 1491
Size: 313 65 1113
Fragments arranged by size:
1113 313 65
PstI C_TGCA'G
Cuts at: 0 126 1491
Size: 126 1365
PvuII CAG'CTG
Cuts at: 0 995 1203 1429 1491
Size: 995 208 226 62
Fragments arranged by size:
995 226 208 62
SacII CC_GC'GG
Cuts at: 0 782 1147 1491
Size: 782 365 344
Fragments arranged by size:
782 365 344
SanDI GG'GwC_CC
Cuts at: 0 378 1491
Size: 378 1113
SapI GCTCTTCn'nnn_
Cuts at: 0 525 1491
Size: 525 966
SfcI C'TryA_G
Cuts at: 0 122 1491
Size: 122 1369
SfoI GGC'GCC
Cuts at: 0 33 1491
Size: 33 1458
SmaI CCC'GGG
Cuts at: 0 15 1491
Size: 15 1476
SmlI C'TyrA_G
Cuts at: 0 663 1491
Size: 663 828
SphI G_CATG'C
Cuts at: 0 1234 1491
Size: 1234 257
SrfI GCCC'GGGC
Cuts at: 0 15 1491
Size: 15 1476
StyI C'CwwG_G
Cuts at: 0 521 1491
Size: 521 970
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 860 1491
Size: 860 631
TatI w'GTAC_w
Cuts at: 0 437 1491
Size: 437 1054
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 297 329 753 785 1491
Size: 297 32 424 32 706
Fragments arranged by size:
706 424 297 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 130 1450 1491
Size: 130 1320 41
Fragments arranged by size:
1320 130 41
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 99 355 1491
Size: 99 256 1136
Fragments arranged by size:
1136 256 99
XmaI C'CCGG_G
Cuts at: 0 13 1491
Size: 13 1478
ZraI GAC'GTC
Cuts at: 0 903 1491
Size: 903 588
Enzymes that do cut:
AatII AcuI AflII AflIII AhdI AleI AloI AlwNI
ApaLI AvaI BanI BanII BbeI BbsI BglI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI
BseRI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BsrBI
BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI
BtsI CspCI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII HpaI KasI MmeI MscI
MslI MspA1I NarI NcoI NdeI NspI PasI PciI
PfoI PmlI PpuMI PstI PvuII SacII SanDI SapI
SfcI SfoI SmaI SmlI SphI SrfI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AgeI AlfI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI
BpuEI BsaBI BsaWI BseYI BsmBI BspEI BspHI BspMI
BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII KpnI MfeI MluI NaeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SspI StuI SwaI XbaI XhoI XmnI