Restriction map for
pF1KB8138
(Linear) (Six-base) MAPSORT of: KIBB8138.seq Check: 4304 from: 1 to: 1779
KIBB8138.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 737 1468 1779
Size: 721 16 731 311
Fragments arranged by size:
731 721 311 16
AccI GT'mk_AC
Cuts at: 0 894 1779
Size: 894 885
Acc65I G'GTAC_C
Cuts at: 0 997 1705 1779
Size: 997 708 74
Fragments arranged by size:
997 708 74
AflIII A'CryG_T
Cuts at: 0 979 1223 1225 1779
Size: 979 244 2 554
Fragments arranged by size:
979 554 244 2
AhdI GACnn_n'nnGTC
Cuts at: 0 633 1779
Size: 633 1146
AleI CACnn'nnGTG
Cuts at: 0 314 1541 1779
Size: 314 1227 238
Fragments arranged by size:
1227 314 238
AlwNI CAG_nnn'CTG
Cuts at: 0 122 248 1112 1180 1776 1779
Size: 122 126 864 68 596 3
Fragments arranged by size:
864 596 126 122 68 3
ApaI G_GGCC'C
Cuts at: 0 1016 1779
Size: 1016 763
ApaLI G'TGCA_C
Cuts at: 0 484 1779
Size: 484 1295
AvaI C'yCGr_G
Cuts at: 0 1371 1779
Size: 1371 408
BanI G'GyrC_C
Cuts at: 0 56 364 502 547 641 997 1023 1664
Size: 56 308 138 45 94 356 26 641
Cuts at: 1664 1705 1779
Size: 41 74
Fragments arranged by size:
641 356 308 138 94 74 56 45
41 26
BanII G_rGCy'C
Cuts at: 0 1016 1779
Size: 1016 763
BbeI G_GCGC'C
Cuts at: 0 1668 1779
Size: 1668 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 482 1779
Size: 482 1297
BbvCI CC'TCA_GC
Cuts at: 0 1054 1235 1779
Size: 1054 181 544
Fragments arranged by size:
1054 544 181
BglI GCCn_nnn'nGGC
Cuts at: 0 302 875 1217 1297 1451 1779
Size: 302 573 342 80 154 328
Fragments arranged by size:
573 342 328 302 154 80
Bme1580I G_kGCm'C
Cuts at: 0 61 379 488 550 646 1016 1028 1779
Size: 61 318 109 62 96 370 12 751
Fragments arranged by size:
751 370 318 109 96 62 61 12
BmgBI CAC'GTC
Cuts at: 0 1145 1554 1779
Size: 1145 409 225
Fragments arranged by size:
1145 409 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 668 1779
Size: 668 1111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1779
Size: 1297 482
Bpu10I CC'TnA_GC
Cuts at: 0 1054 1235 1779
Size: 1054 181 544
Fragments arranged by size:
1054 544 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 1503 1779
Size: 1503 276
BsaAI yAC'GTr
Cuts at: 0 413 980 1226 1539 1779
Size: 413 567 246 313 240
Fragments arranged by size:
567 413 313 246 240
BsaBI GATnn'nnATC
Cuts at: 0 284 1779
Size: 284 1495
BsaHI Gr'CG_yC
Cuts at: 0 1646 1665 1742 1779
Size: 1646 19 77 37
Fragments arranged by size:
1646 77 37 19
BsaWI w'CCGG_w
Cuts at: 0 624 1779
Size: 624 1155
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 119 799 1110 1779
Size: 119 680 311 669
Fragments arranged by size:
680 669 311 119
BseYI C'CCAG_C
Cuts at: 0 17 1044 1388 1505 1619 1736 1758 1779
Size: 17 1027 344 117 114 117 22 21
Fragments arranged by size:
1027 344 117 117 114 22 21 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 715 1779
Size: 338 377 1064
Fragments arranged by size:
1064 377 338
BsiHKAI G_wGCw'C
Cuts at: 0 488 1779
Size: 488 1291
BsmI GAATG_Cn'
Cuts at: 0 400 1779
Size: 400 1379
BsmBI CGTCTCn'nnnn_
Cuts at: 0 234 1779
Size: 234 1545
Bsp1286I G_dGCh'C
Cuts at: 0 61 379 488 550 646 1016 1028 1779
Size: 61 318 109 62 96 370 12 751
Fragments arranged by size:
751 370 318 109 96 62 61 12
BspEI T'CCGG_A
Cuts at: 0 624 1779
Size: 624 1155
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 667 721 737 892 1138 1372 1468 1603
Size: 667 54 16 155 246 234 96 135
Cuts at: 1603 1779
Size: 176
Fragments arranged by size:
667 246 234 176 155 135 96 54
16
BsrFI r'CCGG_y
Cuts at: 0 205 1779
Size: 205 1574
BstBI TT'CG_AA
Cuts at: 0 392 1779
Size: 392 1387
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1521 1779
Size: 1521 258
BtgI C'CryG_G
Cuts at: 0 266 298 359 542 800 968 1779
Size: 266 32 61 183 258 168 811
Fragments arranged by size:
811 266 258 183 168 61 32
BtsI GCAGTG_nn'
Cuts at: 0 195 288 309 321 486 602 732 957
Size: 195 93 21 12 165 116 130 225
Cuts at: 957 1203 1249 1437 1668 1779
Size: 246 46 188 231 111
Fragments arranged by size:
246 231 225 195 188 165 130 116
111 93 46 21 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1058 1093 1779
Size: 1058 35 686
Fragments arranged by size:
1058 686 35
DraIII CAC_nnn'GTG
Cuts at: 0 755 834 1023 1779
Size: 755 79 189 756
Fragments arranged by size:
756 755 189 79
EaeI y'GGCC_r
Cuts at: 0 207 282 1779
Size: 207 75 1497
Fragments arranged by size:
1497 207 75
EarI CTCTTCn'nnn_
Cuts at: 0 1075 1779
Size: 1075 704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1779
Size: 1297 482
EcoNI CCTnn'n_nnAGG
Cuts at: 0 784 1779
Size: 784 995
EcoO109I rG'GnC_Cy
Cuts at: 0 905 1012 1013 1041 1728 1755 1779
Size: 905 107 1 28 687 27 24
Fragments arranged by size:
905 687 107 28 27 24 1
FspI TGC'GCA
Cuts at: 0 827 1695 1720 1779
Size: 827 868 25 59
Fragments arranged by size:
868 827 59 25
HaeII r_GCGC'y
Cuts at: 0 1668 1779
Size: 1668 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 150 182 313 345 619 651 1779
Size: 150 32 131 32 274 32 1128
Fragments arranged by size:
1128 274 150 131 32 32 32
KasI G'GCGC_C
Cuts at: 0 1664 1779
Size: 1664 115
KpnI G_GTAC'C
Cuts at: 0 1001 1709 1779
Size: 1001 708 70
Fragments arranged by size:
1001 708 70
MscI TGG'CCA
Cuts at: 0 284 1779
Size: 284 1495
MslI CAynn'nnrTG
Cuts at: 0 170 314 859 1541 1779
Size: 170 144 545 682 238
Fragments arranged by size:
682 545 238 170 144
MspA1I CmG'CkG
Cuts at: 0 119 530 970 1112 1346 1691 1773 1779
Size: 119 411 440 142 234 345 82 6
Fragments arranged by size:
440 411 345 234 142 119 82 6
NaeI GCC'GGC
Cuts at: 0 207 1779
Size: 207 1572
NarI GG'CG_CC
Cuts at: 0 1665 1779
Size: 1665 114
NgoMIV G'CCGG_C
Cuts at: 0 205 1779
Size: 205 1574
NspI r_CATG'y
Cuts at: 0 275 1779
Size: 275 1504
PasI CC'CwG_GG
Cuts at: 0 1215 1779
Size: 1215 564
PflMI CCAn_nnn'nTGG
Cuts at: 0 558 1779
Size: 558 1221
PmlI CAC'GTG
Cuts at: 0 413 1226 1539 1779
Size: 413 813 313 240
Fragments arranged by size:
813 413 313 240
PpuMI rG'GwC_Cy
Cuts at: 0 1755 1779
Size: 1755 24
PspOMI G'GGCC_C
Cuts at: 0 1012 1779
Size: 1012 767
PstI C_TGCA'G
Cuts at: 0 316 1779
Size: 316 1463
PvuII CAG'CTG
Cuts at: 0 119 1112 1346 1773 1779
Size: 119 993 234 427 6
Fragments arranged by size:
993 427 234 119 6
SacII CC_GC'GG
Cuts at: 0 971 1779
Size: 971 808
SanDI GG'GwC_CC
Cuts at: 0 1755 1779
Size: 1755 24
SexAI A'CCwGG_T
Cuts at: 0 515 1019 1779
Size: 515 504 760
Fragments arranged by size:
760 515 504
SfcI C'TryA_G
Cuts at: 0 114 312 895 1779
Size: 114 198 583 884
Fragments arranged by size:
884 583 198 114
SfoI GGC'GCC
Cuts at: 0 1666 1779
Size: 1666 113
SmlI C'TyrA_G
Cuts at: 0 1764 1779
Size: 1764 15
SphI G_CATG'C
Cuts at: 0 275 1779
Size: 275 1504
StyI C'CwwG_G
Cuts at: 0 785 1779
Size: 785 994
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 9 160 352 555 565 603 1369 1531
Size: 9 151 192 203 10 38 766 162
Cuts at: 1531 1779
Size: 248
Fragments arranged by size:
766 248 203 192 162 151 38 10
9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 290 322 1543 1575 1779
Size: 290 32 1221 32 204
Fragments arranged by size:
1221 290 204 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1144 1350 1779
Size: 1144 206 429
Fragments arranged by size:
1144 429 206
Enzymes that do cut:
AarI AccI Acc65I AflIII AhdI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrFI BstBI BstXI BtgI BtsI
CspCI DraIII EaeI EarI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KasI KpnI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PflMI PmlI PpuMI PspOMI
PstI PvuII SacII SanDI SexAI SfcI SfoI SmlI
SphI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI
BsaXI BsiEI BsiWI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI MfeI MluI MmeI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI