Restriction map for
pF1KB8142
(Linear) (Six-base) MAPSORT of: KIBB8142.seq Check: 5179 from: 1 to: 1182
KIBB8142.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 615 1182
Size: 615 567
AccI GT'mk_AC
Cuts at: 0 95 1182
Size: 95 1087
Acc65I G'GTAC_C
Cuts at: 0 506 545 1182
Size: 506 39 637
Fragments arranged by size:
637 506 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1182
Size: 544 638
AflIII A'CryG_T
Cuts at: 0 947 1182
Size: 947 235
AlwNI CAG_nnn'CTG
Cuts at: 0 41 522 942 1182
Size: 41 481 420 240
Fragments arranged by size:
481 420 240 41
ApaI G_GGCC'C
Cuts at: 0 892 1109 1182
Size: 892 217 73
Fragments arranged by size:
892 217 73
BanI G'GyrC_C
Cuts at: 0 483 506 545 1182
Size: 483 23 39 637
Fragments arranged by size:
637 483 39 23
BanII G_rGCy'C
Cuts at: 0 293 892 1109 1182
Size: 293 599 217 73
Fragments arranged by size:
599 293 217 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 804 1182
Size: 83 721 378
Fragments arranged by size:
721 378 83
Bme1580I G_kGCm'C
Cuts at: 0 278 486 892 1109 1182
Size: 278 208 406 217 73
Fragments arranged by size:
406 278 217 208 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 1182
Size: 309 873
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 432 757 1029 1121 1182
Size: 271 161 325 272 92 61
Fragments arranged by size:
325 272 271 161 92 61
Bpu10I CC'TnA_GC
Cuts at: 0 190 1182
Size: 190 992
BsaAI yAC'GTr
Cuts at: 0 62 948 1182
Size: 62 886 234
Fragments arranged by size:
886 234 62
BsaHI Gr'CG_yC
Cuts at: 0 360 1152 1182
Size: 360 792 30
Fragments arranged by size:
792 360 30
BsaWI w'CCGG_w
Cuts at: 0 353 1182
Size: 353 829
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 448 1182
Size: 418 30 734
Fragments arranged by size:
734 418 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1066 1182
Size: 1066 116
BseYI C'CCAG_C
Cuts at: 0 201 293 341 380 497 558 1182
Size: 201 92 48 39 117 61 624
Fragments arranged by size:
624 201 117 92 61 48 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1182
Size: 928 254
BsmBI CGTCTCn'nnnn_
Cuts at: 0 351 1182
Size: 351 831
Bsp1286I G_dGCh'C
Cuts at: 0 278 293 486 892 1109 1182
Size: 278 15 193 406 217 73
Fragments arranged by size:
406 278 217 193 73 15
BspEI T'CCGG_A
Cuts at: 0 353 1182
Size: 353 829
BspHI T'CATG_A
Cuts at: 0 989 1182
Size: 989 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 615 689 740 1182
Size: 615 74 51 442
Fragments arranged by size:
615 442 74 51
BsrFI r'CCGG_y
Cuts at: 0 194 1182
Size: 194 988
BssSI C'ACGA_G
Cuts at: 0 466 1182
Size: 466 716
BstAPI GCAn_nnn'nTGC
Cuts at: 0 942 1182
Size: 942 240
BstEII G'GTnAC_C
Cuts at: 0 44 1182
Size: 44 1138
BstYI r'GATC_y
Cuts at: 0 848 1182
Size: 848 334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 360 1182
Size: 360 822
BtsI GCAGTG_nn'
Cuts at: 0 574 1182
Size: 574 608
DraIII CAC_nnn'GTG
Cuts at: 0 642 1182
Size: 642 540
EaeI y'GGCC_r
Cuts at: 0 87 149 308 1145 1182
Size: 87 62 159 837 37
Fragments arranged by size:
837 159 87 62 37
EarI CTCTTCn'nnn_
Cuts at: 0 427 796 1182
Size: 427 369 386
Fragments arranged by size:
427 386 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 432 544 757 1029 1121 1182
Size: 271 161 112 213 272 92 61
Fragments arranged by size:
272 271 213 161 112 92 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 736 1182
Size: 736 446
EcoO109I rG'GnC_Cy
Cuts at: 0 888 889 1105 1182
Size: 888 1 216 77
Fragments arranged by size:
888 216 77 1
FspI TGC'GCA
Cuts at: 0 688 973 1182
Size: 688 285 209
Fragments arranged by size:
688 285 209
HaeII r_GCGC'y
Cuts at: 0 139 1182
Size: 139 1043
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 818 850 1182
Size: 818 32 332
Fragments arranged by size:
818 332 32
HincII GTy'rAC
Cuts at: 0 756 1182
Size: 756 426
KpnI G_GTAC'C
Cuts at: 0 510 549 1182
Size: 510 39 633
Fragments arranged by size:
633 510 39
MscI TGG'CCA
Cuts at: 0 151 1182
Size: 151 1031
MslI CAynn'nnrTG
Cuts at: 0 477 655 1182
Size: 477 178 527
Fragments arranged by size:
527 477 178
MspA1I CmG'CkG
Cuts at: 0 297 384 410 939 1099 1182
Size: 297 87 26 529 160 83
Fragments arranged by size:
529 297 160 87 83 26
NspI r_CATG'y
Cuts at: 0 765 978 1182
Size: 765 213 204
Fragments arranged by size:
765 213 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1182
Size: 642 540
PmlI CAC'GTG
Cuts at: 0 62 948 1182
Size: 62 886 234
Fragments arranged by size:
886 234 62
PspOMI G'GGCC_C
Cuts at: 0 888 1105 1182
Size: 888 217 77
Fragments arranged by size:
888 217 77
PstI C_TGCA'G
Cuts at: 0 335 938 1082 1182
Size: 335 603 144 100
Fragments arranged by size:
603 335 144 100
PvuII CAG'CTG
Cuts at: 0 297 384 410 939 1099 1182
Size: 297 87 26 529 160 83
Fragments arranged by size:
529 297 160 87 83 26
SfcI C'TryA_G
Cuts at: 0 331 669 934 981 1078 1182
Size: 331 338 265 47 97 104
Fragments arranged by size:
338 331 265 104 97 47
SphI G_CATG'C
Cuts at: 0 978 1182
Size: 978 204
StuI AGG'CCT
Cuts at: 0 742 1182
Size: 742 440
StyI C'CwwG_G
Cuts at: 0 284 1182
Size: 284 898
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 66 193 444 859 1182
Size: 66 127 251 415 323
Fragments arranged by size:
415 323 251 127 66
TatI w'GTAC_w
Cuts at: 0 536 1182
Size: 536 646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 494 536 1053 1182
Size: 246 248 42 517 129
Fragments arranged by size:
517 248 246 129 42
XmnI GAAnn'nnTTC
Cuts at: 0 259 1182
Size: 259 923
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlwNI ApaI BanI
BanII BbsI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspEI
BspHI BspMI BsrFI BssSI BstAPI BstEII BstYI BtgZI
BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KpnI MscI MslI MspA1I NspI
PflMI PmlI PspOMI PstI PvuII SfcI SphI StuI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaBI BsiEI BsiHKAI BsiWI BsmI BsrBI BsrDI BsrGI
BssHII BstBI BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KasI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI