Restriction map for
pF1KB8143
(Linear) (Six-base) MAPSORT of: KIBB8143.seq Check: 4623 from: 1 to: 1218
KIBB8143.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 277 1218
Size: 277 941
Acc65I G'GTAC_C
Cuts at: 0 178 1218
Size: 178 1040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1218
Size: 604 614
AflIII A'CryG_T
Cuts at: 0 479 1218
Size: 479 739
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 679 711 1218
Size: 679 32 507
Fragments arranged by size:
679 507 32
AlwNI CAG_nnn'CTG
Cuts at: 0 68 525 1218
Size: 68 457 693
Fragments arranged by size:
693 457 68
ApaI G_GGCC'C
Cuts at: 0 866 1218
Size: 866 352
AvaI C'yCGr_G
Cuts at: 0 865 1051 1069 1084 1218
Size: 865 186 18 15 134
Fragments arranged by size:
865 186 134 18 15
BanI G'GyrC_C
Cuts at: 0 178 529 733 740 1218
Size: 178 351 204 7 478
Fragments arranged by size:
478 351 204 178 7
BanII G_rGCy'C
Cuts at: 0 809 866 1076 1218
Size: 809 57 210 142
Fragments arranged by size:
809 210 142 57
BbeI G_GCGC'C
Cuts at: 0 533 737 744 1218
Size: 533 204 7 474
Fragments arranged by size:
533 474 204 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1107 1218
Size: 1107 111
BlpI GC'TnA_GC
Cuts at: 0 721 1218
Size: 721 497
Bme1580I G_kGCm'C
Cuts at: 0 866 1218
Size: 866 352
BmgBI CAC'GTC
Cuts at: 0 75 1218
Size: 75 1143
BmrI ACTGGGnnnn_n'
Cuts at: 0 410 1218
Size: 410 808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 595 814 1000 1218
Size: 114 481 219 186 218
Fragments arranged by size:
481 219 218 186 114
Bpu10I CC'TnA_GC
Cuts at: 0 200 1218
Size: 200 1018
BsaI GGTCTCn'nnnn_
Cuts at: 0 640 1218
Size: 640 578
BsaHI Gr'CG_yC
Cuts at: 0 455 530 734 741 964 1218
Size: 455 75 204 7 223 254
Fragments arranged by size:
455 254 223 204 75 7
BsaWI w'CCGG_w
Cuts at: 0 167 426 1218
Size: 167 259 792
Fragments arranged by size:
792 259 167
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 679 709 1218
Size: 679 30 509
Fragments arranged by size:
679 509 30
BseYI C'CCAG_C
Cuts at: 0 1063 1075 1123 1218
Size: 1063 12 48 95
Fragments arranged by size:
1063 95 48 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1218
Size: 1078 140
BsiEI CG_ry'CG
Cuts at: 0 778 1109 1211 1218
Size: 778 331 102 7
Fragments arranged by size:
778 331 102 7
BsiHKAI G_wGCw'C
Cuts at: 0 809 986 1218
Size: 809 177 232
Fragments arranged by size:
809 232 177
BsmBI CGTCTCn'nnnn_
Cuts at: 0 571 1218
Size: 571 647
Bsp1286I G_dGCh'C
Cuts at: 0 809 866 986 1076 1218
Size: 809 57 120 90 142
Fragments arranged by size:
809 142 120 90 57
BspEI T'CCGG_A
Cuts at: 0 426 1218
Size: 426 792
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 277 1218
Size: 277 941
BsrBI CCG'CTC
Cuts at: 0 705 1218
Size: 705 513
BsrFI r'CCGG_y
Cuts at: 0 13 649 689 1218
Size: 13 636 40 529
Fragments arranged by size:
636 529 40 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 990 1218
Size: 990 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 1218
Size: 573 645
BtgI C'CryG_G
Cuts at: 0 401 1218
Size: 401 817
BtsI GCAGTG_nn'
Cuts at: 0 372 414 1218
Size: 372 42 804
Fragments arranged by size:
804 372 42
EaeI y'GGCC_r
Cuts at: 0 395 775 1106 1208 1218
Size: 395 380 331 102 10
Fragments arranged by size:
395 380 331 102 10
EagI C'GGCC_G
Cuts at: 0 775 1106 1208 1218
Size: 775 331 102 10
Fragments arranged by size:
775 331 102 10
EcoICRI GAG'CTC
Cuts at: 0 807 1218
Size: 807 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 595 604 814 1000 1218
Size: 114 481 9 210 186 218
Fragments arranged by size:
481 218 210 186 114 9
EcoO109I rG'GnC_Cy
Cuts at: 0 65 895 1218
Size: 65 830 323
Fragments arranged by size:
830 323 65
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 177 609 1218
Size: 177 432 609
Fragments arranged by size:
609 432 177
FspI TGC'GCA
Cuts at: 0 189 1218
Size: 189 1029
HaeII r_GCGC'y
Cuts at: 0 476 533 696 728 737 744 1218
Size: 476 57 163 32 9 7 474
Fragments arranged by size:
476 474 163 57 32 9 7
HincII GTy'rAC
Cuts at: 0 1152 1218
Size: 1152 66
KasI G'GCGC_C
Cuts at: 0 529 733 740 1218
Size: 529 204 7 478
Fragments arranged by size:
529 478 204 7
KpnI G_GTAC'C
Cuts at: 0 182 1218
Size: 182 1036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1218
Size: 464 754
MslI CAynn'nnrTG
Cuts at: 0 271 1218
Size: 271 947
MspA1I CmG'CkG
Cuts at: 0 573 636 720 780 990 1023 1218
Size: 573 63 84 60 210 33 195
Fragments arranged by size:
573 210 195 84 63 60 33
NaeI GCC'GGC
Cuts at: 0 15 1218
Size: 15 1203
NarI GG'CG_CC
Cuts at: 0 530 734 741 1218
Size: 530 204 7 477
Fragments arranged by size:
530 477 204 7
NgoMIV G'CCGG_C
Cuts at: 0 13 1218
Size: 13 1205
NotI GC'GGCC_GC
Cuts at: 0 775 1218
Size: 775 443
NspI r_CATG'y
Cuts at: 0 663 1218
Size: 663 555
PasI CC'CwG_GG
Cuts at: 0 729 969 1218
Size: 729 240 249
Fragments arranged by size:
729 249 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 525 1218
Size: 525 693
PfoI T'CCnGG_A
Cuts at: 0 32 958 1218
Size: 32 926 260
Fragments arranged by size:
926 260 32
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 679 711 1218
Size: 316 32 331 32 507
Fragments arranged by size:
507 331 316 32 32
PspOMI G'GGCC_C
Cuts at: 0 862 1218
Size: 862 356
PvuII CAG'CTG
Cuts at: 0 573 636 720 1218
Size: 573 63 84 498
Fragments arranged by size:
573 498 84 63
SacI G_AGCT'C
Cuts at: 0 809 1218
Size: 809 409
ScaI AGT'ACT
Cuts at: 0 856 1218
Size: 856 362
SexAI A'CCwGG_T
Cuts at: 0 221 1218
Size: 221 997
SfoI GGC'GCC
Cuts at: 0 531 735 742 1218
Size: 531 204 7 476
Fragments arranged by size:
531 476 204 7
SmaI CCC'GGG
Cuts at: 0 867 1053 1086 1218
Size: 867 186 33 132
Fragments arranged by size:
867 186 132 33
SrfI GCCC'GGGC
Cuts at: 0 1086 1218
Size: 1086 132
StyI C'CwwG_G
Cuts at: 0 458 698 1218
Size: 458 240 520
Fragments arranged by size:
520 458 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 621 961 1061 1137 1218
Size: 130 491 340 100 76 81
Fragments arranged by size:
491 340 130 100 81 76
TatI w'GTAC_w
Cuts at: 0 854 1218
Size: 854 364
XmaI C'CCGG_G
Cuts at: 0 865 1051 1084 1218
Size: 865 186 33 134
Fragments arranged by size:
865 186 134 33
XmnI GAAnn'nnTTC
Cuts at: 0 124 160 262 331 1218
Size: 124 36 102 69 887
Fragments arranged by size:
887 124 102 69 36
Enzymes that do cut:
AarI Acc65I AcuI AflIII AloI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BstAPI BstXI BtgI BtsI EaeI EagI EcoICRI Eco57MI
EcoO109I FalI FspI HaeII HincII KasI KpnI MmeI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI
PflMI PfoI PpiI PspOMI PvuII SacI ScaI SexAI
SfoI SmaI SrfI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmtI BplI BpuEI BsaAI BsaBI BseRI BsiWI BsmI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoNI EcoRI EcoRV FseI FspAI Hin4I
HindIII HpaI MfeI MluI MscI NcoI NdeI NheI
NruI NsiI PacI PciI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI ZraI