(Linear) (Six-base) MAPSORT of: KIBB8144.seq  Check: 6027  from: 1  to: 807



KIBB8144.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      31     807 
   Size:         31     776
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     807 
   Size:         34     773
 
AleI CACnn'nnGTG

Cuts at:      0     555     807 
   Size:        555     252
 
ApoI r'AATT_y

Cuts at:      0     260     658     807 
   Size:        260     398     149

  Fragments arranged by size: 

                 398     260     149
 
AvaI C'yCGr_G

Cuts at:      0     135     807 
   Size:        135     672
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     357     807 
   Size:        357     450
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     526     560     807 
   Size:        526      34     247

  Fragments arranged by size: 

                 526     247      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     110     807 
   Size:        110     697
 
BglII A'GATC_T

Cuts at:      0     630     807 
   Size:        630     177
 
BmgBI CAC'GTC

Cuts at:      0      44     557     807 
   Size:         44     513     250

  Fragments arranged by size: 

                 513     250      44
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     164     807 
   Size:        164     643
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     293     807 
   Size:        293     514
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     807 
   Size:        167     640
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     581     807 
   Size:        581     226
 
BseYI C'CCAG_C

Cuts at:      0       6     807 
   Size:          6     801
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     807 
   Size:        566     241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      31     807 
   Size:         31     776
 
BsrDI GCAATG_nn'

Cuts at:      0      93     807 
   Size:         93     714
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      32     807 
   Size:         32     775
 
BstYI r'GATC_y

Cuts at:      0      80     630     807 
   Size:         80     550     177

  Fragments arranged by size: 

                 550     177      80
 
BtsI GCAGTG_nn'

Cuts at:      0     410     807 
   Size:        410     397
 
DraI TTT'AAA

Cuts at:      0     363     807 
   Size:        363     444
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      45     807 
   Size:         45     762
 
EaeI y'GGCC_r

Cuts at:      0     102     807 
   Size:        102     705
 
EarI CTCTTCn'nnn_

Cuts at:      0     535     807 
   Size:        535     272
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     195     807 
   Size:        195     612
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     167     807 
   Size:         34     133     640

  Fragments arranged by size: 

                 640     133      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     503     535     589     621     807 
   Size:        503      32      54      32     186

  Fragments arranged by size: 

                 503     186      54      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     555     807 
   Size:        555     252
 
MspA1I CmG'CkG

Cuts at:      0       6     122     164     807 
   Size:          6     116      42     643

  Fragments arranged by size: 

                 643     116      42       6
 
NdeI CA'TA_TG

Cuts at:      0     745     807 
   Size:        745      62
 
PfoI T'CCnGG_A

Cuts at:      0     521     807 
   Size:        521     286
 
PstI C_TGCA'G

Cuts at:      0      28     417     807 
   Size:         28     389     390

  Fragments arranged by size: 

                 390     389      28
 
SfcI C'TryA_G

Cuts at:      0      24     413     807 
   Size:         24     389     394

  Fragments arranged by size: 

                 394     389      24
 
StyI C'CwwG_G

Cuts at:      0     637     807 
   Size:        637     170
 
SwaI ATTT'AAAT

Cuts at:      0     363     807 
   Size:        363     444
 
Tth111I GACn'n_nGTC

Cuts at:      0      42     807 
   Size:         42     765
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      97     807 
   Size:         97     710
 
XmnI GAAnn'nnTTC

Cuts at:      0     632     807 
   Size:        632     175
 

 Enzymes that do cut:

     AarI     AcuI     AleI     ApoI     AvaI     BbsI     BcgI     BglI
    BglII    BmgBI     BmrI     BplI     BpmI     BsaI    BseYI     BsgI
    BspMI    BsrDI   BstAPI    BstYI     BtsI     DraI     DrdI     EaeI
     EarI     EciI  Eco57MI    Hin4I     MslI   MspA1I     NdeI     PfoI
     PstI     SfcI     StyI     SwaI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
    BciVI     BclI    BfrBI     BlpI Bme1580I     BmtI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI   DraIII     EagI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI    TaqII     TatI
     TstI     XbaI     XhoI     XmaI     ZraI