(Linear) (Six-base) MAPSORT of: KIBB8145.seq  Check: 8692  from: 1  to: 924



KIBB8145.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0     207     924 
   Size:        207     717
 
AflIII A'CryG_T

Cuts at:      0     332     924 
   Size:        332     592
 
AleI CACnn'nnGTG

Cuts at:      0     904     924 
   Size:        904      20
 
AlwNI CAG_nnn'CTG

Cuts at:      0     235     924 
   Size:        235     689
 
ApoI r'AATT_y

Cuts at:      0     472     924 
   Size:        472     452
 
AvaI C'yCGr_G

Cuts at:      0      58     924 
   Size:         58     866
 
BanI G'GyrC_C

Cuts at:      0     532     676     688     898     924 
   Size:        532     144      12     210      26

  Fragments arranged by size: 

                 532     210     144      26      12
 
BanII G_rGCy'C

Cuts at:      0      59     924 
   Size:         59     865
 
BbvCI CC'TCA_GC

Cuts at:      0     104     924 
   Size:        104     820
 
Bme1580I G_kGCm'C

Cuts at:      0     679     901     924 
   Size:        679     222      23

  Fragments arranged by size: 

                 679     222      23
 
BmgBI CAC'GTC

Cuts at:      0     707     924 
   Size:        707     217
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     778     924 
   Size:        778     146
 
Bpu10I CC'TnA_GC

Cuts at:      0     104     924 
   Size:        104     820
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     281     924 
   Size:        281     643
 
BsaBI GATnn'nnATC

Cuts at:      0     123     924 
   Size:        123     801
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     924 
   Size:        121     803
 
BseYI C'CCAG_C

Cuts at:      0     800     924 
   Size:        800     124
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      32     924 
   Size:         32     892
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     679     901     924 
   Size:         59     620     222      23

  Fragments arranged by size: 

                 620     222      59      23
 
BssSI C'ACGA_G

Cuts at:      0     301     924 
   Size:        301     623
 
BstEII G'GTnAC_C

Cuts at:      0     849     924 
   Size:        849      75
 
BstYI r'GATC_y

Cuts at:      0     353     401     924 
   Size:        353      48     523

  Fragments arranged by size: 

                 523     353      48
 
Bsu36I CC'TnA_GG

Cuts at:      0     245     924 
   Size:        245     679
 
BtgI C'CryG_G

Cuts at:      0     564     924 
   Size:        564     360
 
BtsI GCAGTG_nn'

Cuts at:      0     722     924 
   Size:        722     202
 
EarI CTCTTCn'nnn_

Cuts at:      0     748     924 
   Size:        748     176
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     131     924 
   Size:        131     793
 
HaeII r_GCGC'y

Cuts at:      0     863     924 
   Size:        863      61
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     924 
   Size:         46      32     846

  Fragments arranged by size: 

                 846      46      32
 
HindIII A'AGCT_T

Cuts at:      0     409     924 
   Size:        409     515
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     924 
   Size:        194     730
 
MslI CAynn'nnrTG

Cuts at:      0     904     924 
   Size:        904      20
 
MspA1I CmG'CkG

Cuts at:      0     736     838     866     924 
   Size:        736     102      28      58

  Fragments arranged by size: 

                 736     102      58      28
 
NcoI C'CATG_G

Cuts at:      0     564     924 
   Size:        564     360
 
NspI r_CATG'y

Cuts at:      0     336     924 
   Size:        336     588
 
PciI A'CATG_T

Cuts at:      0     332     924 
   Size:        332     592
 
PshAI GACnn'nnGTC

Cuts at:      0     880     924 
   Size:        880      44
 
PvuII CAG'CTG

Cuts at:      0     736     838     924 
   Size:        736     102      86

  Fragments arranged by size: 

                 736     102      86
 
StyI C'CwwG_G

Cuts at:      0     564     602     924 
   Size:        564      38     322

  Fragments arranged by size: 

                 564     322      38
 
XmnI GAAnn'nnTTC

Cuts at:      0     323     924 
   Size:        323     601
 

 Enzymes that do cut:

     AccI   AflIII     AleI    AlwNI     ApoI     AvaI     BanI    BanII
    BbvCI Bme1580I    BmgBI     BmrI   Bpu10I     BsaI    BsaBI    BseRI
    BseYI    BsmBI Bsp1286I    BssSI   BstEII    BstYI   Bsu36I     BtgI
     BtsI     EarI     EciI    HaeII    Hin4I  HindIII     MmeI     MslI
   MspA1I     NcoI     NspI     PciI    PshAI    PvuII     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmtI     BplI     BpmI    BpuEI
    BsaAI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI    BstXI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI