(Linear) (Six-base) MAPSORT of: KIBB8152.seq  Check: 1530  from: 1  to: 618



KIBB8152.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     618 
   Size:        303     315
 
ApoI r'AATT_y

Cuts at:      0      27     521     618 
   Size:         27     494      97

  Fragments arranged by size: 

                 494      97      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     548     618 
   Size:        548      70
 
BlpI GC'TnA_GC

Cuts at:      0     437     618 
   Size:        437     181
 
BsaAI yAC'GTr

Cuts at:      0     459     618 
   Size:        459     159
 
BsaBI GATnn'nnATC

Cuts at:      0     193     618 
   Size:        193     425
 
BseYI C'CCAG_C

Cuts at:      0     208     618 
   Size:        208     410
 
BssSI C'ACGA_G

Cuts at:      0     479     618 
   Size:        479     139
 
BtsI GCAGTG_nn'

Cuts at:      0      69     448     618 
   Size:         69     379     170

  Fragments arranged by size: 

                 379     170      69
 
ClaI AT'CG_AT

Cuts at:      0     191     618 
   Size:        191     427
 
EarI CTCTTCn'nnn_

Cuts at:      0     413     565     618 
   Size:        413     152      53

  Fragments arranged by size: 

                 413     152      53
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     618 
   Size:        303     315
 
EcoRI G'AATT_C

Cuts at:      0      27     618 
   Size:         27     591
 
EcoRV GAT'ATC

Cuts at:      0     195     618 
   Size:        195     423
 
HincII GTy'rAC

Cuts at:      0     453     492     618 
   Size:        453      39     126

  Fragments arranged by size: 

                 453     126      39
 
HpaI GTT'AAC

Cuts at:      0     453     618 
   Size:        453     165
 
MslI CAynn'nnrTG

Cuts at:      0      55     618 
   Size:         55     563
 
MspA1I CmG'CkG

Cuts at:      0     208     313     618 
   Size:        208     105     305

  Fragments arranged by size: 

                 305     208     105
 
PvuII CAG'CTG

Cuts at:      0     208     313     618 
   Size:        208     105     305

  Fragments arranged by size: 

                 305     208     105
 
SfcI C'TryA_G

Cuts at:      0      94     308     413     618 
   Size:         94     214     105     205

  Fragments arranged by size: 

                 214     205     105      94
 
StyI C'CwwG_G

Cuts at:      0      17     618 
   Size:         17     601
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI     BlpI    BsaAI    BsaBI    BseYI    BssSI
     BtsI     ClaI     EarI  Eco57MI    EcoRI    EcoRV   HincII     HpaI
     MslI   MspA1I    PvuII     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI