Restriction map for
pF1KB8154
(Linear) (Six-base) MAPSORT of: KIBB8154.seq Check: 6924 from: 1 to: 1707
KIBB8154.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AclI AA'CG_TT
Cuts at: 0 742 1707
Size: 742 965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 897 1149 1233 1707
Size: 813 84 252 84 474
Fragments arranged by size:
813 474 252 84 84
ApaLI G'TGCA_C
Cuts at: 0 1595 1707
Size: 1595 112
ApoI r'AATT_y
Cuts at: 0 39 84 259 695 1031 1115 1451 1535
Size: 39 45 175 436 336 84 336 84
Cuts at: 1535 1619 1707
Size: 84 88
Fragments arranged by size:
436 336 336 175 88 84 84 84
45 39
AseI AT'TA_AT
Cuts at: 0 424 1707
Size: 424 1283
BanII G_rGCy'C
Cuts at: 0 191 673 1707
Size: 191 482 1034
Fragments arranged by size:
1034 482 191
BbsI GAAGACnn'nnnn_
Cuts at: 0 365 651 1707
Size: 365 286 1056
Fragments arranged by size:
1056 365 286
BciVI GTATCCnnnnn_n'
Cuts at: 0 495 1087 1707
Size: 495 592 620
Fragments arranged by size:
620 592 495
BclI T'GATC_A
Cuts at: 0 1269 1707
Size: 1269 438
BfrBI ATG'CAT
Cuts at: 0 721 1707
Size: 721 986
BlpI GC'TnA_GC
Cuts at: 0 1019 1707
Size: 1019 688
Bme1580I G_kGCm'C
Cuts at: 0 1599 1707
Size: 1599 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 341 1707
Size: 341 1366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 809 893 977 1145 1229 1313 1397
Size: 725 84 84 84 168 84 84 84
Cuts at: 1397 1481 1565 1707
Size: 84 84 142
Fragments arranged by size:
725 168 142 84 84 84 84 84
84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1707
Size: 32 1675
BsaI GGTCTCn'nnnn_
Cuts at: 0 957 1125 1707
Size: 957 168 582
Fragments arranged by size:
957 582 168
BsaBI GATnn'nnATC
Cuts at: 0 1274 1707
Size: 1274 433
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 128 158 1707
Size: 128 30 1549
Fragments arranged by size:
1549 128 30
BseYI C'CCAG_C
Cuts at: 0 393 1707
Size: 393 1314
BsiHKAI G_wGCw'C
Cuts at: 0 673 1599 1707
Size: 673 926 108
Fragments arranged by size:
926 673 108
BsmI GAATG_Cn'
Cuts at: 0 843 1431 1707
Size: 843 588 276
Fragments arranged by size:
843 588 276
Bsp1286I G_dGCh'C
Cuts at: 0 191 673 1599 1707
Size: 191 482 926 108
Fragments arranged by size:
926 482 191 108
BspHI T'CATG_A
Cuts at: 0 1611 1707
Size: 1611 96
BsrGI T'GTAC_A
Cuts at: 0 89 1104 1707
Size: 89 1015 603
Fragments arranged by size:
1015 603 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1183 1435 1603 1707
Size: 1183 252 168 104
Fragments arranged by size:
1183 252 168 104
BstBI TT'CG_AA
Cuts at: 0 971 1707
Size: 971 736
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 1707
Size: 12 1695
BstYI r'GATC_y
Cuts at: 0 1513 1707
Size: 1513 194
DraI TTT'AAA
Cuts at: 0 249 1707
Size: 249 1458
EarI CTCTTCn'nnn_
Cuts at: 0 689 1707
Size: 689 1018
EcoICRI GAG'CTC
Cuts at: 0 671 1707
Size: 671 1036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 809 813 893 897 977 1145 1149
Size: 725 84 4 80 4 80 168 4
Cuts at: 1149 1229 1233 1313 1397 1481 1565 1707
Size: 80 4 80 84 84 84 142
Fragments arranged by size:
725 168 142 84 84 84 84 80
80 80 80 4 4 4 4
EcoRI G'AATT_C
Cuts at: 0 695 1031 1115 1451 1535 1619 1707
Size: 695 336 84 336 84 84 88
Fragments arranged by size:
695 336 336 88 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 463 1707
Size: 463 1244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 924 956 1707
Size: 148 32 744 32 751
Fragments arranged by size:
751 744 148 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 1707
Size: 86 1621
MslI CAynn'nnrTG
Cuts at: 0 554 1707
Size: 554 1153
MspA1I CmG'CkG
Cuts at: 0 393 1707
Size: 393 1314
NdeI CA'TA_TG
Cuts at: 0 282 1707
Size: 282 1425
NsiI A_TGCA'T
Cuts at: 0 723 1707
Size: 723 984
NspI r_CATG'y
Cuts at: 0 725 1707
Size: 725 982
PvuII CAG'CTG
Cuts at: 0 393 1707
Size: 393 1314
SacI G_AGCT'C
Cuts at: 0 673 1707
Size: 673 1034
SfcI C'TryA_G
Cuts at: 0 114 1506 1691 1707
Size: 114 1392 185 16
Fragments arranged by size:
1392 185 114 16
SmlI C'TyrA_G
Cuts at: 0 47 1707
Size: 47 1660
StuI AGG'CCT
Cuts at: 0 994 1330 1433 1707
Size: 994 336 103 274
Fragments arranged by size:
994 336 274 103
StyI C'CwwG_G
Cuts at: 0 5 1707
Size: 5 1702
TatI w'GTAC_w
Cuts at: 0 89 1104 1707
Size: 89 1015 603
Fragments arranged by size:
1015 603 89
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 230 1707
Size: 230 1477
XmnI GAAnn'nnTTC
Cuts at: 0 446 658 742 826 1162 1246 1582 1707
Size: 446 212 84 84 336 84 336 125
Fragments arranged by size:
446 336 336 212 125 84 84 84
Enzymes that do cut:
AclI AcuI ApaLI ApoI AseI BanII BbsI BciVI
BclI BfrBI BlpI Bme1580I BmrI BpmI BpuEI BsaI
BsaBI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrGI
BstAPI BstBI BstXI BstYI DraI EarI EcoICRI Eco57MI
EcoRI FalI Hin4I MmeI MslI MspA1I NdeI NsiI
NspI PvuII SacI SfcI SmlI StuI StyI TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BglI BglII BmgBI BmtI BplI Bpu10I BsaAI BsaHI
BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstEII BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI ZraI