(Linear) (Six-base) MAPSORT of: KIBB8157.seq  Check: 526  from: 1  to: 1647



KIBB8157.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0    1262    1576    1647 
   Size:       1262     314      71

  Fragments arranged by size: 

                1262     314      71
 
Acc65I G'GTAC_C

Cuts at:      0     546    1647 
   Size:        546    1101
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1614    1647 
   Size:       1614      33
 
ApaI G_GGCC'C

Cuts at:      0    1336    1647 
   Size:       1336     311
 
ApoI r'AATT_y

Cuts at:      0     372     558    1077    1087    1647 
   Size:        372     186     519      10     560

  Fragments arranged by size: 

                 560     519     372     186      10
 
AvrII C'CTAG_G

Cuts at:      0     130    1647 
   Size:        130    1517
 
BanI G'GyrC_C

Cuts at:      0     546    1186    1647 
   Size:        546     640     461

  Fragments arranged by size: 

                 640     546     461
 
BanII G_rGCy'C

Cuts at:      0    1336    1647 
   Size:       1336     311
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1358    1647 
   Size:       1358     289
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     861    1647 
   Size:        861     786
 
BglII A'GATC_T

Cuts at:      0     540    1647 
   Size:        540    1107
 
BlpI GC'TnA_GC

Cuts at:      0     191    1647 
   Size:        191    1456
 
Bme1580I G_kGCm'C

Cuts at:      0    1336    1647 
   Size:       1336     311
 
Bpu10I CC'TnA_GC

Cuts at:      0     196    1509    1647 
   Size:        196    1313     138

  Fragments arranged by size: 

                1313     196     138
 
BsaBI GATnn'nnATC

Cuts at:      0     904    1647 
   Size:        904     743
 
BsaWI w'CCGG_w

Cuts at:      0    1369    1647 
   Size:       1369     278
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1218    1248    1531    1561    1647 
   Size:       1218      30     283      30      86

  Fragments arranged by size: 

                1218     283      86      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     939    1220    1223    1459    1567    1647 
   Size:        939     281       3     236     108      80

  Fragments arranged by size: 

                 939     281     236     108      80       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     162    1647 
   Size:        162    1485
 
BsmI GAATG_Cn'

Cuts at:      0     678    1647 
   Size:        678     969
 
Bsp1286I G_dGCh'C

Cuts at:      0     162    1336    1647 
   Size:        162    1174     311

  Fragments arranged by size: 

                1174     311     162
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      49    1555    1647 
   Size:         49    1506      92

  Fragments arranged by size: 

                1506      92      49
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      41    1647 
   Size:         41    1606
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1132    1647 
   Size:       1132     515
 
BstYI r'GATC_y

Cuts at:      0     453     540    1647 
   Size:        453      87    1107

  Fragments arranged by size: 

                1107     453      87
 
Bsu36I CC'TnA_GG

Cuts at:      0     476    1647 
   Size:        476    1171
 
BtgI C'CryG_G

Cuts at:      0     854    1647 
   Size:        854     793
 
EaeI y'GGCC_r

Cuts at:      0    1343    1647 
   Size:       1343     304
 
EarI CTCTTCn'nnn_

Cuts at:      0     182     479    1107    1523    1604    1647 
   Size:        182     297     628     416      81      43

  Fragments arranged by size: 

                 628     416     297     182      81      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1614    1647 
   Size:       1614      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1024    1647 
   Size:       1024     623
 
EcoO109I rG'GnC_Cy

Cuts at:      0     714    1332    1333    1647 
   Size:        714     618       1     314

  Fragments arranged by size: 

                 714     618     314       1
 
EcoRI G'AATT_C

Cuts at:      0     372    1077    1647 
   Size:        372     705     570

  Fragments arranged by size: 

                 705     570     372
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     733    1647 
   Size:        733     914
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1218    1250    1531    1563    1647 
   Size:       1218      32     281      32      84

  Fragments arranged by size: 

                1218     281      84      32      32
 
HincII GTy'rAC

Cuts at:      0    1570    1647 
   Size:       1570      77
 
KpnI G_GTAC'C

Cuts at:      0     550    1647 
   Size:        550    1097
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     750    1647 
   Size:        422     328     897

  Fragments arranged by size: 

                 897     422     328
 
MscI TGG'CCA

Cuts at:      0    1345    1647 
   Size:       1345     302
 
NcoI C'CATG_G

Cuts at:      0     854    1647 
   Size:        854     793
 
NspI r_CATG'y

Cuts at:      0     876    1518    1647 
   Size:        876     642     129

  Fragments arranged by size: 

                 876     642     129
 
PspOMI G'GGCC_C

Cuts at:      0    1332    1647 
   Size:       1332     315
 
PstI C_TGCA'G

Cuts at:      0    1527    1647 
   Size:       1527     120
 
SapI GCTCTTCn'nnn_

Cuts at:      0     182    1647 
   Size:        182    1465
 
ScaI AGT'ACT

Cuts at:      0    1049    1647 
   Size:       1049     598
 
SfcI C'TryA_G

Cuts at:      0    1071    1523    1559    1647 
   Size:       1071     452      36      88

  Fragments arranged by size: 

                1071     452      88      36
 
SphI G_CATG'C

Cuts at:      0     876    1647 
   Size:        876     771
 
StuI AGG'CCT

Cuts at:      0       9    1647 
   Size:          9    1638
 
StyI C'CwwG_G

Cuts at:      0     130     854     887    1647 
   Size:        130     724      33     760

  Fragments arranged by size: 

                 760     724     130      33
 
TatI w'GTAC_w

Cuts at:      0     386    1047    1633    1647 
   Size:        386     661     586      14

  Fragments arranged by size: 

                 661     586     386      14
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     ApaI     ApoI    AvrII     BanI    BanII
     BbsI    BciVI    BglII     BlpI Bme1580I   Bpu10I    BsaBI    BsaWI
    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BspMI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    Hin4I   HincII     KpnI     MmeI     MscI     NcoI
     NspI   PspOMI     PstI     SapI     ScaI     SfcI     SphI     StuI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     MfeI     MluI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI